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CC BY-NC-ND 4.0 · European Journal of General Dentistry 2013; 2(02): 134-138
DOI: 10.4103/2278-9626.112311
Original Article

In vitro antimicrobial efficiency of different root canal sealers against Enterecoccus faecalis

Yakup Ustun
Department of Restorative Dentistry and Endodontics, Faculty of Dentistry, Erciyes University, Kayseri, Turkey
,
Burak Sagsen
Department of Restorative Dentistry and Endodontics, Faculty of Dentistry, Erciyes University, Kayseri, Turkey
,
Suleyman Durmaz
1   Department of Clinical Microbiology and Infectious Diseases, Düziçi Public Hospital, Osmaniye, Turkey
,
Duygu Percin
2   Department of Medical Microbiology, Faculty of Medicine, Erciyes University, Kayseri, Turkey
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Abstract

Objective: The antibacterial effectiveness of four different sealers AH Plus, EndoRez, mineral trioxide aggregate (MTA) Fillapex, iRoot SP against Enterococcus faecalis was evaluated by time kill assay method in vitro. Materials and Methods: Four sealers are used in this study: An epoxy resin-based sealer, AH Plus (Dentsply, Maillefer, Switzerland), a polymethacrylate resin-based sealer, EndoRez (Ultradent, South Jordan, UT) and two calcium silicate-based sealers, MTA Fillapex (Angelus Solucxoes Odontologicas, Londrina, Brazil), iRoot SP (Innovative BioCreamix Inc., Vancouver, Canada). Each sealer was mixed according to manufacturer′s instructions. Five mg of each sealer was added to sterile tubes separately and evaluated at 20 min, 24 h, 7 days, and 30 days. Two tubes were used as positive and negative. Results: At the 20th min AH Plus and iRoot SP were bactericidal, MTA Fillapex, and EndoRez were ineffective at the 20th min. At the 1st day MTA Fillapex was ineffective and rest of the sealers was bacteriostatic. At the 7th day, only MTA Fillapex showed bactericidal effect, AH Plus, iRoot SP and EndoRez were bacteriostatic. At the 30th day, MTA Fillapex was still bactericidal, AH Plus, iRoot SP, and EndoRez were still bacteriostatic. Conclusion: All root canal sealers tested were effective against E. faecalis. Fresh iRoot SP and fresh AH Plus had bactericidal action against E. faecalis. EndoRez has bacteriostatic behavior against E. faecalis. MTA Fillapex was the only sealer that could be bacteriocidal at 7th and 30th day.

Keywords

Antibacterial activity - Enterococcus faecalis - mineral trioxide aggregate Fillapex - root canal sealer - time kill assay


Publication History

Article published online:
01 November 2021

© 2013. European Journal of General Dentistry. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/.)

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