In vitro antimicrobial efficiency of different root canal sealers against Enterecoccus faecalis

Yakup Ustun; Burak Sagsen; Suleyman Durmaz; Duygu Percin

doi:10.4103/2278-9626.112311

European Journal of General Dentistry, Table of Contents

CC BY-NC-ND 4.0 · European Journal of General Dentistry 2013; 2(02): 134-138
DOI: 10.4103/2278-9626.112311

Original Article

In vitro antimicrobial efficiency of different root canal sealers against Enterecoccus faecalis

Yakup Ustun

Department of Restorative Dentistry and Endodontics, Faculty of Dentistry, Erciyes University, Kayseri, Turkey

,

Burak Sagsen

Department of Restorative Dentistry and Endodontics, Faculty of Dentistry, Erciyes University, Kayseri, Turkey

,

Suleyman Durmaz

¹Department of Clinical Microbiology and Infectious Diseases, Düziçi Public Hospital, Osmaniye, Turkey

,

Duygu Percin

²Department of Medical Microbiology, Faculty of Medicine, Erciyes University, Kayseri, Turkey

› Author Affiliations

Abstract

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Abstract

Objective: The antibacterial effectiveness of four different sealers AH Plus, EndoRez, mineral trioxide aggregate (MTA) Fillapex, iRoot SP against Enterococcus faecalis was evaluated by time kill assay method in vitro. Materials and Methods: Four sealers are used in this study: An epoxy resin-based sealer, AH Plus (Dentsply, Maillefer, Switzerland), a polymethacrylate resin-based sealer, EndoRez (Ultradent, South Jordan, UT) and two calcium silicate-based sealers, MTA Fillapex (Angelus Solucxoes Odontologicas, Londrina, Brazil), iRoot SP (Innovative BioCreamix Inc., Vancouver, Canada). Each sealer was mixed according to manufacturer′s instructions. Five mg of each sealer was added to sterile tubes separately and evaluated at 20 min, 24 h, 7 days, and 30 days. Two tubes were used as positive and negative. Results: At the 20^th min AH Plus and iRoot SP were bactericidal, MTA Fillapex, and EndoRez were ineffective at the 20^th min. At the 1^st day MTA Fillapex was ineffective and rest of the sealers was bacteriostatic. At the 7^th day, only MTA Fillapex showed bactericidal effect, AH Plus, iRoot SP and EndoRez were bacteriostatic. At the 30^th day, MTA Fillapex was still bactericidal, AH Plus, iRoot SP, and EndoRez were still bacteriostatic. Conclusion: All root canal sealers tested were effective against E. faecalis. Fresh iRoot SP and fresh AH Plus had bactericidal action against E. faecalis. EndoRez has bacteriostatic behavior against E. faecalis. MTA Fillapex was the only sealer that could be bacteriocidal at 7^th and 30^th day.

Keywords

Antibacterial activity - Enterococcus faecalis - mineral trioxide aggregate Fillapex - root canal sealer - time kill assay

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References

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