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CC BY-NC-ND 4.0 · J Lab Physicians 2019; 11(01): 045-050
DOI: 10.4103/JLP.JLP_107_18
Original Article

Typing of Neisseria gonorrhoeae isolates by phenotypic and genotypic techniques in New Delhi, India

Seema Sood
Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India
,
Neeraj Mahajan
Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India
,
Rajendra Singh
Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India
,
Sonu Kumari Agrawal
Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India
,
Trupti Shende
Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India
,
Arti Kapil
Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India
,
Hemanta K. Kar
Department of Dermatology, STDs and Leprosy, PGIMER and Dr. Ram Manohar Lohia Hospital, New Delhi, India
,
Vinod K. Sharma
Department of Dermatology and Venereology, All India Institute of Medical Sciences, New Delhi, India
› Author Affiliations Financial support and sponsorship: This work was financially supported by the ICMR, Government of India [grant no. IRIS-ID No. 2006-0685E]; and the All India Institute of Medical Sciences [project no.F.5-59/IRG/2010/RS].
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Abstract

BACKGROUND: The objective of this study is to investigate gonococcal isolates using phenotypic and genotypic methods.

METHODOLOGY: Sixty gonococcal isolates obtained were examined. Strains were divided into 9 resistant phenotypes: Chromosomally mediated penicillin-resistant Neisseria gonorrhoeae (CMRNGP), penicillinase-producing NG (PPNG), chromosomally mediated tetracycline-resistant NG (CMRNGT), TRNG, PPNG and TRNG, CMRNGPT, quinolone resistant NG (QRNG), Azithro R, and decreased susceptibility (DS) to ceftriaxone. These isolates were also subjected to auxotyping and NG-multi-antigen sequence typing (MAST).

RESULTS: Of 60 isolates, 32 (53.33%) PPNG and only one was CMRNGP; 16 (26.66%) were CMRNGT, while 18 (30%) were TRNG. Both PPNG and TRNG found in 13 (21.66%) and none were CMRNGPT. QRNG was seen in 93.33%, 5% Azithromycin R, and 6.66% were DS to ceftriaxone. Based on auxotyping, 24 (40%) nonrequiring, 16 (26.66%) were proline requiring, 13 (21.66%) arginine requiring while 7 (11.66%) belonged to others. The most common ST was 6058 (32.5%). The discriminatory indices of antibiogram, auxotyping and NG-MAST were 0.77, 0.72, and 0.95, respectively.

CONCLUSIONS: NG-MAST is the method of choice for epidemiological studies.

Key words

Antibiogram - auxotyping - Neisseria gonorrhoeae multiantigen sequence typing - Neisseria gonorrhoeae


Publication History

Received: 06 August 2018

Accepted: 26 August 2018

Article published online:
06 April 2020

© 2019.

Thieme Medical and Scientific Publishers Private Ltd.
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