Occurrence of bla genes encoding carbapenem-resistant Pseudomonas aeruginosa and Acinetobacter baumannii from Intensive Care Unit in a tertiary care hospital

Jayanthi Siva Subramaniyan; Jeya Meenakshi Sundaram

doi:10.4103/JLP.JLP_108_17

Journal of Laboratory Physicians, Table of Contents

CC BY-NC-ND 4.0 · J Lab Physicians 2018; 10(02): 208-213
DOI: 10.4103/JLP.JLP_108_17

Original Article

Occurrence of bla genes encoding carbapenem-resistant Pseudomonas aeruginosa and Acinetobacter baumannii from Intensive Care Unit in a tertiary care hospital

Authors

Jayanthi Siva Subramaniyan

Department of Microbiology, Chettinad Hospital and Research Institute, Kanchipuram, Tamil Nadu, India
Jeya Meenakshi Sundaram

Department of Microbiology, Chettinad Hospital and Research Institute, Kanchipuram, Tamil Nadu, India

Abstract

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Abstract

CONTEXT: ICU shows increasing incidence of infection associated with the use of invasive procedures for the diagnostic purpose as well as the indiscriminate use of antibiotics. Pseudomonas aeruginosa and Acinetobacter species are "very successful" pathogen and the emergence of the Metallo-β-Lactamases (MBL) is becoming a therapeutic challenge.

AIMS: To isolate the Nonfermenting Gram negative bacilli from the ICU samples. To identify the metallo betalactamase producers and to detect the bla gene presence among the Pseudomonas aeruginosa and Acinetobacter baumannii.

SETTINGS AND DESIGN: The Nonfermenting Gram negative bacilli isolates from the ICU samples were taken over for 5 years (2009-2014) in a tertiary care hospital.

METHODS AND MATERIALS: The isolates of Pseudomonas species and Acinetobacter species were confirmed by API analyser and processed according to standard procedures. Detection of the MBL producers were done by E strip method and subjected for bla gene detection by PCR method.

RESULTS: In our study a total of 195 isolates of NFGNB were obtained from various ICU. Of these MBL producers, 26 % were Pseudomonas aeruginosa and 25 % were Acinetobacter baumannii. The subtypes of bla _VIMMBL producing P.aeruginosa were 26%.The predominant gene coding for MBL activity in A.baumannii were found to be bla _OXA gene 11.9%. The gene accession numbers were KF975367, KF975372.

CONCLUSIONS: We have to control the development and dissemination of these superbugs among the ICU's.

Keywords

Acinetobacter baumanni - bla genes - ICU - Metallo-β-lactamases - Pseudomonas aeruginosa

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References

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