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CC BY-NC-ND 4.0 · J Lab Physicians 2019; 11(02): 138-143
DOI: 10.4103/JLP.JLP_118_18
Original Article

Detection of VIM and NDM-1 metallo-beta-lactamase genes in carbapenem-resistant Pseudomonas aeruginosa clinical strains in Bahrain

Ronni Mol Joji
Department of Microbiology, Immunology and Infectious Disease, College of Medicine and Medical Sciences, Arabian Gulf University, Manama, India
,
Nouf Al-Rashed
Department of Microbiology, Immunology and Infectious Disease, College of Medicine and Medical Sciences, Arabian Gulf University, Manama, India
,
Nermin Kamal Saeed
Department of Pathology, Salmaniya Medical Complex, Ministry of Health, Manama, Kingdom of Bahrain, India
,
Khalid Mubarak Bindayna
Department of Microbiology, Immunology and Infectious Disease, College of Medicine and Medical Sciences, Arabian Gulf University, Manama, India
› Author Affiliations Financial support and sponsorship This study was supported by Arabian Gulf University.
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Abstract

INTRODUCTION: Carbapenem-resistant Pseudomonas aeruginosa has emerged as a life-threatening infectious agent worldwide. Carbapenemase genes are reported to be some of the most common mechanisms for carbapenem resistance in P. aeruginosa. No reports are available from the Kingdom of Bahrain about carbapenem resistance and the underlying cause. In this study, we determined to study the presence of the metallo-beta-lactamase (M β L) genes of VIM family and NDM-1 in carbapenem-resistant P. aeruginosa strains.

METHODOLOGY: Fifty carbapenem-resistant P. aeruginosa isolates were obtained from three main hospitals of Bahrain. They were subjected to antimicrobial susceptibility testing by disc diffusion test. Subsequently, MβL was detected by imipenem-ethylene diamine tetraacetic acid (EDTA) combined disc test and conventional polymerase chain reaction.

RESULTS: Among 50 P. aeruginosa strains, 40 (80%) were imipenem resistant. Among the 40 imipenem-resistant strains, 35 (87.5%) strains were positive for the imipenem-EDTA combined disc test, and 21 (52%) were carrying MβL genes. Nineteen (47.5%) strains were positive for the VIM gene; one (2.5%) strain was carrying the NDM-1 gene, while one strain was carrying both the VIM and NDM-1 genes. None of the imipenem sensitive strains carried the VIM or NDM-1 gene.

CONCLUSION: This is the first study to report the presence of the VIM family gene and NDM-1 genes in imipenem-resistant P. aeruginosa isolates in the Kingdom of Bahrain. The study also confirms the multiple drug resistance by the MβL strains, attention should therefore from now on, be focused on prevention of further spread of such isolates by firm infection control measures, and to reduce its threat to public health.

Key words

Carbapenem resistance - NDM-1 gene - Pseudomonas aeruginosa - VIM gene


Publication History

Received: 13 September 2018

Accepted: 03 January 2019

Article published online:
06 April 2020

© 2019.

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  • References

  • 1 Kateete DP, Nakanjako R, Namugenyi J, Erume J, Joloba ML, Najjuka CF, et al. Carbapenem resistant Pseudomonas aeruginosa and Acinetobacter baumannii at mulago hospital in Kampala, Uganda (2007-2009). Springerplus 2016;5:1308.
  • 2 Rizek C, Fu L, Dos Santos LC, Leite G, Ramos J, Rossi F, et al. Characterization of carbapenem-resistant Pseudomonas aeruginosa clinical isolates, carrying multiple genes coding for this antibiotic resistance. Ann Clin Microbiol Antimicrob 2014;13:43.
  • 3 Manenzhe RI, Zar HJ, Nicol MP, Kaba M. The spread of carbapenemase-producing bacteria in Africa: A systematic review. J Antimicrob Chemother 2015;70:23-40.
  • 4 Qu TT, Zhang JL, Wang J, Tao J, Yu YS, Chen YG, et al. Evaluation of phenotypic tests for detection of metallo-beta-lactamase-producing Pseudomonas aeruginosa strains in china. J Clin Microbiol 2009;47:1136-42.
  • 5 Walsh TR, Toleman MA, Poirel L, Nordmann P. Metallo-beta-lactamases: The quiet before the storm? Clin Microbiol Rev 2005;18:306-25.
  • 6 Elsherif R, Ismail D, Elawady S, Jastaniah S, Al-Masaudi S, Harakeh S,et al. Boronic acid disk diffusion for the phenotypic detection of polymerase chain reaction-confirmed, carbapenem-resistant, gram-negative bacilli isolates. BMC Microbiol 2016;16:135.
  • 7 Yong D, Lee K, Yum JH, Shin HB, Rossolini GM, Chong Y, et al. Imipenem-EDTA disk method for differentiation of metallo-beta-lactamase-producing clinical isolates of Pseudomonas spp. And Acinetobacter spp. J Clin Microbiol 2002;40:3798-801.
  • 8 EL-Mosallamy W, Osman A, Tabl H, AL-Tabbakh A. Phenotypic and genotypic methods for detection of metallo-beta-lactamase (MBL) producing Pseudomonas aeruginosa. Egypt J Med Microbiol 2015;24:27-35.
  • 9 Ahmed OB, Dablool AS. Quality improvement of the DNA extracted by boiling method in gram negative bacteria. Int J Bioassays 2017;6:5347-9.
  • 10 Hammami S, Boutiba-Ben Boubaker I, Ghozzi R, Saidani M, Amine S, Ben Redjeb S, et al. Nosocomial outbreak of imipenem-resistant Pseudomonas aeruginosa producing VIM-2 metallo-β-lactamase in a kidney transplantation unit. Diagn Pathol 2011;6:106.
  • 11 Lister PD, Wolter DJ, Hanson ND. Antibacterial-resistant Pseudomonas aeruginosa: Clinical impact and complex regulation of chromosomally encoded resistance mechanisms. Clin Microbiol Rev 2009;22:582-610.
  • 12 Polotto M, Casella T, de Lucca Oliveira MG, Rúbio FG, Nogueira ML, de Almeida MT, et al. Detection of P. Aeruginosa harboring bla CTX-M-2, bla GES-1 and bla GES-5, bla IMP-1 and bla SPM-1 causing infections in Brazilian tertiary-care hospital. BMC Infect Dis 2012;12:176.
  • 13 Arunagiri K, Sekar B, Sangeetha G, John J. Detection and characterization of metallo-beta-lactamases in Pseudomonas aeruginosa by phenotypic and molecular methods from clinical samples in a tertiary care hospital. West Indian Med J 2012;61:778-83.
  • 14 Mohamed AA, Shibl AM, Zaki SA, Tawfik AF. Antimicrobial resistance pattern and prevalence of metallo-β-lactamases in Pseudomonas aeruginosa from Saudi Arabia. Afr J Microbiol Res 2011;5:5528-33.
  • 15 Al-Agamy MH, Jeannot K, El-Mahdy TS, Samaha HA, Shibl AM, Plésiat P, et al. Diversity of molecular mechanisms conferring carbapenem resistance to Pseudomonas aeruginosa isolates from Saudi Arabia. Can J Infect Dis Med Microbiol 2016;2016:4379686.
  • 16 Pitout JD, Gregson DB, Poirel L, McClure JA, Le P, Church DL, et al. Detection of Pseudomonas aeruginosa producing metallo-beta-lactamases in a large centralized laboratory. J Clin Microbiol 2005;43:3129-35.
  • 17 Saderi H, Lotfalipour H, Owlia P, Salimi H. Detection of Metallo-β-Lactamase Producing Pseudomonas aeruginosa Isolated From Burn Patients in Tehran, Iran. Lab Med 2010;41:609-12.
  • 18 Panchal CA, Oza SS, Mehta SJ. Comparison of four phenotypic methods for detection of metallo-β-lactamase-producing gram-negative bacteria in rural teaching hospital. J Lab Physicians 2017;9:81-3.
  • 19 Al-Agamy MH, Shibl AM, Zaki SA, Tawfik AF. Antimicrobial resistance pattern and prevalence of MBL in P. aeruginosa from Saudi Arabia. Afr J Microbiol Res 2011;30:5528-33.
  • 20 Tawfik AF, Shibl AM, Aljohi MA, Altammami MA, Al-Agamy MH. Distribution of ambler class A, B and D β-lactamases among Pseudomonas aeruginosa isolates. Burns 2012;38:855-60.
  • 21 Dortet L, Poirel L, Nordmann P. Worldwide dissemination of the NDM-type carbapenemases in gram-negative bacteria. Biomed Res Int 2014;2014:249856.
  • 22 Zafer MM, Al-Agamy MH, El-Mahallawy HA, Amin MA, Ashour MS. Antimicrobial resistance pattern and their beta-lactamase encoding genes among Pseudomonas aeruginosa strains isolated from cancer patients. Biomed Res Int 2014;2014:101635.
  • 23 Shanthi M, Sekar U, Kamalanathan A, Sekar B. Detection of new delhi metallo beta lactamase-1 (NDM-1) carbapenemase in Pseudomonas aeruginosa in a single centre in southern india. Indian J Med Res 2014;140:546-50.
  • 24 Shaaban M, Al-Qahtani A, Al-Ahdal M, Barwa R. Molecular characterization of resistance mechanisms in Pseudomonas aeruginosa isolates resistant to carbapenems. J Infect Dev Ctries 2017;11:935-43.
  • 25 Correa A, Montealegre MC, Mojica MF, Maya JJ, Rojas LJ, De La Cadena EP, et al. First report of a Pseudomonas aeruginosa isolate coharboring KPC and VIM carbapenemases. Antimicrob Agents Chemother 2012;56:5422-3.
  • 26 Pandya N, Prajapati S, Mehta S, Kikani K, Joshi P. Evaluation of various methods for detection of (MβL) production in gram negative bacilli. Egypt J Med Microbiol 2011;2:775-7.
  • 27 Picão RC, Andrade SS, Nicoletti AG, Campana EH, Moraes GC, Mendes RE, et al. Metallo-beta-lactamase detection: Comparative evaluation of double-disk synergy versus combined disk tests for IMP-, GIM-, SIM-, SPM-, or VIM-producing isolates. J Clin Microbiol 2008;46:2028-37.