Detection of carbapenemase-producing Pseudomonas aeruginosa by phenotypic and genotypic methods in a tertiary care hospital of East India

Nishu Verma; Ashok Prahraj; Baijayantimala Mishra; Bijayini Behera; Kavita Gupta

doi:10.4103/JLP.JLP_136_19

Journal of Laboratory Physicians, Table of Contents

CC BY-NC-ND 4.0 · J Lab Physicians 2019; 11(04): 287-291
DOI: 10.4103/JLP.JLP_136_19

Original Article

Detection of carbapenemase-producing Pseudomonas aeruginosa by phenotypic and genotypic methods in a tertiary care hospital of East India

Authors

Nishu Verma

Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, Odisha, India
Ashok Prahraj

Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, Odisha, India
Baijayantimala Mishra

Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, Odisha, India
Bijayini Behera

Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, Odisha, India
Kavita Gupta

Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, Odisha, India

Abstract

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Abstract

BACKGROUND: Carbapenemase-producing Pseudomonas aeruginosa is a serious threat in hospital infection due to its multidrug resistance.

AIM: The aim of the study was to determine the frequency of carbapenem resistance in clinical isolates of Pseudomonas aeruginosa and detect the presence of carbapenemase enzymes in carbapenem-resistant P. aeruginosa (CRPA) isolates by phenotypic and genotypic methods.

MATERIAL AND METHODS: Double-disk synergy test [DDST] and combined disk synergy test [CDST]) was performed in CRPA isolates and the prevalence of^blaKPC,^blaNDM-1,^blaIMP,^blaVIM,^blaSIM,^blaSPM,^blaGIM, and^blaOXA-48 was determined.

RESULTS: Of 559 isolates included in the study, a total of 102 isolates were resistant to carbapenem that accounted for overall 18.24% (102/559) prevalence. Of these 102 isolates, 89 (87.25%) isolates were positive by DDST and 95 (93.17%) isolates were positive by CDST. Of 102 CRPA isolates,^blaVIM was detected in 30 isolates (30/102, 29.1%), followed by^blaNDM-1 in 29 (29/102, 28.4%) isolates and^blaSIM and^blaGIM in 6 isolates each (6/102, 5.8%). A combination of two carbapenemase genes was detected in 12 isolates, with six (6/102, 5.88%) CRPA isolates harboring with both^blaVIM and^blaNDM-1 genes. Four isolates were found to harbor a combination of three carbapenem-resistant genes.

CONCLUSION: A high rate of carbapenemase production was observed in P. aeruginosa. Coproducers of multiple carbapenemases are also a cause of concern. An in-depth understanding of molecular mechanisms of resistance will be helpful in optimizing patient management and hospital infection control.

Key words

Carbapenemases - genotypic methods - phenotypic methods - Pseudomonas aeruginosa

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References

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