One-sample two-smear versus two-sample two-smear approach for the diagnosis of pulmonary tuberculosis

T. Jaya Chandra

doi:10.4103/JLP.JLP_145_17

Journal of Laboratory Physicians, Table of Contents

CC BY-NC-ND 4.0 · J Lab Physicians 2018; 10(02): 135-139
DOI: 10.4103/JLP.JLP_145_17

Original Article

One-sample two-smear versus two-sample two-smear approach for the diagnosis of pulmonary tuberculosis

T. Jaya Chandra

Department of Microbiology, GSL Medical College, Rajahmundry, Andhra Pradesh, India

› Author Affiliations

Abstract

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ABSTRACT

BACKGROUND: To evaluate the efficacy of one-sputum sample two-smear approach for the diagnosis of pulmonary tuberculosis (PT).

MATERIALS AND METHODS: Data from January 2012 to December 2015 were analyzed to find (1) number of smear positives (SP_s) by spot (S) sample with one and two smears; (2) number of SP_s by morning (M) sample with one and two smears; and (iii) number of SP_s by two samples with two smears, that is, same-day (SS₂) and spot morning (SM) approaches. The Chi-square test was used to evaluate the statistical difference in SP cases.

RESULTS: With one-sample two-smear approach, the smear positivity (SPT) was 87% and 87.5%, for S and M samples, respectively, for Ziehl–Neelsen (ZN) staining; whereas, SPT was 96% and 97%, respectively, for S and M samples, for fluorescent staining (FS) technique. With two-sample two-smear approach, for ZN staining, SPT was 89% each and for FS technique, SPT was 97% and 99%, respectively, for SS₂ and SM approaches. The difference was not statistically significant (P > 0.05) between one- and two-sample approaches in the staining techniques.

CONCLUSION: Significant number of SP cases are identified by S sample two-smear approach. Thus, the World Health Organization/Revised National Tuberculosis Control Programme can initiate S sample two-smear approach for the diagnosis of PT.

Key words

Sample - Staining - Tuberculosis

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References

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