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CC BY-NC-ND 4.0 · J Lab Physicians 2018; 10(01): 101-105
DOI: 10.4103/JLP.JLP_25_17
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In vitro cost-effective methods to detect carbapenemases in Enterobacteriaceae

Varsha Gupta
Department of Microbiology, Government Medical College Hospital, Chandigarh, India
,
Ranu Soni
Department of Microbiology, Government Medical College Hospital, Chandigarh, India
,
Neha Jain
Department of Microbiology, Government Medical College Hospital, Chandigarh, India
,
Jagdish Chander
Department of Microbiology, Government Medical College Hospital, Chandigarh, India
› Author Affiliations Financial support and sponsorship: Nil
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Abstract

The rise in carbapenemases-producing organisms has challenged the scientific community. Infections caused by these bacteria have limited treatment options. There are various types such as Klebsiella pneumoniae carbapenemase (Ambler class A), metallo-beta-lactamases of VIM-type, IMP-type, NDM-type (Ambler class B), and OXA-48-types (Ambler class D). An efficient strategy for detection of carbapenemase producers is important to determine the appropriate therapeutic modalities. In this study, four methods - Carba NP test, modified Carba NP (MCNP) test, carbapenem inactivation method (CIM) test, and Rapidec Carba NP kit test were evaluated. We evaluated an in-house MCNP test to detect carbapenemase production using a single protocol which gave reliable results. Furthermore, CIM using routine antibiotic discs gives good results. Both these tests were found to be cost-effective.

Key words

Carbapenemases - Enterobacteriaceae - imipenem


Publication History

Received: 06 February 2017

Accepted: 25 June 2017

Article published online:
19 February 2020

© 2018.

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  • References

  • 1 Queenan AM, Bush K. Carbapenemases: The versatile beta-lactamases. Clin Microbiol Rev 2007;20:440-58.
  • 2 Nordmann P, Poirel L. Strategies for identification of carbapenemase-producing Enterobacteriaceae. J Antimicrob Chemother 2013;68:487-9.
  • 3 Clinical and Laboratory Standards Institute, 950 West Valley Road, Suite 2500, Wayne, Pennsylvania 19087 USA, 2016.
  • 4 Nordmann P, Poirel L, Dortet L. Rapid detection of carbapenemase producing Enterobacteriaceae. Emerg Infect Dis 2012;18:1503-7.
  • 5 Bakour S, Garcia V, Loucif L, Brunel JM, Gharout-Sait A, Touati A, et al. Rapid identification of carbapenemase-producing Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter baumannii using a modified Carba NP test. New Microbes New Infect 2015;7:89-93.
  • 6 van der Zwaluw K, de Haan A, Pluister GN, Bootsma HJ, de Neeling AJ, Schouls LM. The carbapenem inactivation method (CIM), a simple and low-cost alternative for the Carba NP test to assess phenotypic carbapenemase activity in gram-negative rods. PLoS One 2015;10:e0123690.
  • 7 AbdelGhani S, Thomson GK, Snyder JW, Thomson KS. Comparison of the Carba NP, Modified Carba NP, and Updated Rosco Neo-Rapid Carb Kit tests for carbapenemase detection. J Clin Microbiol 2015;53:3539-42.