Co occurrence of two 16S rRNA methyltrasferases along with NDM and OXA 48 like carbapenamases on a single plasmid in Klebsiella pneumoniae

K. V. L. Aishwarya; P. V. Geetha; M. Shanthi; S. Uma

doi:10.4103/JLP.JLP_59_19

Journal of Laboratory Physicians, Table of Contents

CC BY-NC-ND 4.0 · J Lab Physicians 2019; 11(04): 305-311
DOI: 10.4103/JLP.JLP_59_19

Original Article

Co occurrence of two 16S rRNA methyltrasferases along with NDM and OXA 48 like carbapenamases on a single plasmid in Klebsiella pneumoniae

K. V. L. Aishwarya

Department of Microbiology, Sri Ramachandra Institute for Higher Education and Research, Chennai, Tamil Nadu, India

,

P. V. Geetha

Department of Microbiology, Sri Ramachandra Institute for Higher Education and Research, Chennai, Tamil Nadu, India

,

M. Shanthi

Department of Microbiology, Sri Ramachandra Institute for Higher Education and Research, Chennai, Tamil Nadu, India

,

S. Uma

Department of Microbiology, Sri Ramachandra Institute for Higher Education and Research, Chennai, Tamil Nadu, India

› Author Affiliations

Abstract

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Abstract

BACKGROUND: The carbapenemase-encoding genes, bla _NDM- and bla _OXA-48 _- _like, confer resistance to all the known beta-lactams and are encountered along with other beta-lactamase-encoding genes and/or 16S ribosomal RNA (rRNA)-methylating genes. The co-occurrence of bla _NDM and bla _OXA-48 _- _like on a single plasmid is a rare occurrence.

AIM AND OBJECTIVE: The purpose of the study was to characterize the plasmids in Klebsiella pneumoniae isolates producing 16S rRNA methyltransferase along with bla _NDM, bla _OXA-48-like, and other resistance encoding genes.

MATERIALS AND METHODS: One-hundred and seventeen K. pneumoniae clinical isolates which were resistant to aminoglycosides were collected. Polymerase chain reaction-based screening for 16S rRNA methyltransferase genes armA, rmtB, and rmtC; carbapenamase genes bla _NDM, bla _OXA-48-like, bla _IMP, bla _VIM,and bla _KPC; and other resistance genes such as bla _TEM, bla _SHV, bla _CTX-M, and qnr (A, B, and S) determinants acc (6') Ib-cr was performed. Conjugation experiment was carried out for seven isolates that anchored bla _NDMand bla _OXA-48-likealong with any one of the 16S rRNA methyltransferases. The plasmid-based replicon typing for different plasmid-incompatible (Inc) group was performed on the conjugatively transferable plasmids.

RESULTS: Among the 16S rRNA methyltransferases, armA was more predominant. bla _NDMand bla _OXA-48 _-likewere present in 56 (47.86%) and 22 (18.80%) isolates, respectively. Out of seven isolates which were conjugatively transferable, only four had bla _NDMand bla _OXA-48 _- _likeon the same plasmid and they belonged to Inc N and A/C replicon. Three isolates co-harbored 16S rRNA methyltransferases armA, rmtB, and rmtC, and out of the them, one isolate harbored two 16S rRNA methyltransferases armA and rmtB, on the single-plasmid replicon A/C.

CONCLUSION: This is the first report revealing the coexistence of bla _NDMand bla _OXA-48 _- _likeco-harboring two 16S rRNA methylases on a single conjugative plasmid replicon belonging to incompatibility group A/C.

Key words

16S ribosomal RNA methyltransferase - A/C replicon - bla _NDM - bla _{OXA-48 like} - co-harboring - Klebsiella pneumoniae

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References

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