Abnormalities of the intestinal pacemaker cells, enteric neurons, and smooth muscle in intestinal atresia

Radhika krishna OH; Mohammed Abdul Aleem; Geetha Kayla

doi:10.4103/JLP.JLP_94_18

Journal of Laboratory Physicians, Table of Contents

CC BY-NC-ND 4.0 · J Lab Physicians 2019; 11(03): 180-185
DOI: 10.4103/JLP.JLP_94_18

Original Article

Abnormalities of the intestinal pacemaker cells, enteric neurons, and smooth muscle in intestinal atresia

Radhika krishna OH

Department of Pathology, Niloufer Hospital for Women and Children, Hyderabad, Telangana, India

,

Mohammed Abdul Aleem

Department of Pathology, Niloufer Hospital for Women and Children, Hyderabad, Telangana, India

,

Geetha Kayla

Department of Pathology, Niloufer Hospital for Women and Children, Hyderabad, Telangana, India

› Author Affiliations

Abstract

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Abstract

BACKGROUND: Small bowel atresia is a congenital disorder that carves a substantial morbidity. Numerous postoperative gastrointestinal motility problems occur. The underlying cause of this motility disorder is still unclear. Interstitial cells of Cajal (ICC) play a major role in gastrointestinal motility.

AIMS AND OBJECTIVES: To investigate the morphological changes of enteric nervous system and ICC in small bowel atresia.

MATERIAL AND METHODS: Resected small bowel specimen from affected patients (n=15) were divided into three parts (proximal, distal, atretic). Standard histology and immunohistochemistry with anti C-KIT receptor antibody (CD117), calretinin and α-SMA was carried out. The density of myenteric ICCs in the proximal, atretic and distal parts was demonstrated by CD 117 while Calretinin was used for ganglion cells and nerve bundles, α-SMA highlighted muscle hypertrophy.

RESULT AND CONCLUSION: The proximal and distal bowel revealed clear changes in the morphology and density of enteric nervous system and interstitial cells of Cajal..

Key words

C-kit - enteric nervous system - interstitial cells of Cajal - intestinal atresia

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References

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