In vitro comparative evaluation of different storage media (hank's balanced salt solution, propolis, Aloe vera, and pomegranate juice) for preservation of avulsed tooth

Prashant Babaji; Mahesh Melkundi; Raghu Devanna; Suresh B. S.; Vishwajit Rampratap Chaurasia; Gopinath P. V.

doi:10.4103/ejd.ejd_101_16

European Journal of Dentistry, Table of Contents

CC BY-NC-ND 4.0 · Eur J Dent 2017; 11(01): 071-075
DOI: 10.4103/ejd.ejd_101_16

Original Article

Dental Investigation Society

In vitro comparative evaluation of different storage media (hank's balanced salt solution, propolis, Aloe vera, and pomegranate juice) for preservation of avulsed tooth

Prashant Babaji

¹Department of Pedodontics and Preventive Dentistry, Sharavthi Dental College & Hospital, Shimoga, Karnataka, India

,

Mahesh Melkundi

²Department of Oral and Maxillofacial Pathology, College of Dental Sciences, Rau, Indore, Madhya Pradesh, India

,

Raghu Devanna

³Department of Orthodontics, Faculty of Dentistry, Taif University, Kingdom of Saudi Arabiya

,

Suresh B. S.

⁴Department of Pedodontics, Subbaiah Institute of Dental Sciences and Hospital, Shimoga, Karnataka, India

,

Vishwajit Rampratap Chaurasia

⁵Department of Conservative Dentistry and Endodontics, Daswani Dental College, Kota, Rajasthan, India

,

Gopinath P. V.

⁶Department of Periodontics, Malbar DentalCollege and Research Centre, Edappal, Kerala, India

› Author Affiliations

Abstract

ABSTRACT

Objectives: Prognosis of the avulsed teeth is mostly affected by extraoral dry period and storage medium used to store teeth before reimplantation. However, ability of storage media can affect cell viability and success of treatment. Various storage media were tried with some success. The present study was undertaken to comparatively evaluate the efficacy of hank's balanced salt solution (HBSS), propolis, Aloe vera, and pomegranate juice (PJ) in preserving the vitality of periodontal ligament (PDL) cells of avulsed teeth. Materials and Methods: Fifty orthodontically extracted sound teeth with healthy PDL were selected for the present study. Selected teeth were randomly divided into study groups (10 in each) and 5 each as positive and negative control groups. All the teeth were immersed immediately after extraction into respective storage media. Data were statistically analyzed using IBM SPSS software for Windows, Version 19.0., IBM Corp., Armonk, NY, USA. Analysis of variance and multiple range were done using Tukey's honestly significant difference with level of significance at 5% (P > 0.05). Results: Propolis (285,000 viable cells with standard deviation 4.11028 and standard error of 1.38097) showed more viable PDL cells followed by HBSS, A. vera, and PJ. Conclusion: Propolis, A. vera, and PJ can be used as an alternative tooth storage media.

Key words:

Aloe vera - avulsion - periodontal ligament - pomegranate - propolis - storage media - tooth

Full Text

References

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