Open Access
CC BY-NC-ND 4.0 · Eur J Dent 2017; 11(03): 385-389
DOI: 10.4103/ejd.ejd_183_17
Original Article
European Journal of Dentistry

Human salivary protein extraction from RNAPro·SAL™, Pure·SAL™, and passive drooling method

Zohaib Khurshid
1   Prosthodontics and Implantology, College of Dentistry, King Faisal University, Al-Ahsa 31982, KSA
,
Syed Faraz Moin
2   National Centre for Proteomics, University of Karachi, Karachi, Pakistan
,
Rabia Sannam Khan
3   Department of Oral Pathology, College of Dentistry, Baqai University, Karachi, Pakistan
,
Muhammad Atif Saleem Agwan
4   Department of Restorative Dental Sciences, College of Dentistry, Qassim University, Qassim, KSA
,
Abdullah Hamed Alwadaani
1   Prosthodontics and Implantology, College of Dentistry, King Faisal University, Al-Ahsa 31982, KSA
,
Muhammad Sohail Zafar
5   Department of Restorative Dentistry, College of Dentistry, Taibah University, Madinah, Al Munawwarah, Saudi Arabia
› Institutsangaben
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Publikationsverlauf

Publikationsdatum:
25. September 2019 (online)

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ABSTRACT

Objective: The aim of the current study was to carry out a preliminary validation of devices for standardized collection of whole mouth fluid (WMF) in comparison to the passive drooling method for protein analysis in healthy subjects.Materials and Methods: A carefully designed sample collection/pretreatment protocol is crucial to the success of any saliva proteomics project. In this study, WMF was collected from healthy volunteers (n = 10, ages: 18–26 years). Individuals with any oral disease were excluded from the study group. In our study, we evaluated the following collection methods; the classical passive drooling method (unstimulated whole saliva) and standardized tools for saliva collection (Pure·SAL™, and RNAPro·SAL™) from Oasis Diagnostics® Corporation (Vancouver WA, USA). For estimation of protein levels, we used the bicinchoninic acid assay and protein assay kit (Thermo Fisher). The two-dimensional gel electrophoresis sample analysis was carried out for the estimation of proteins in one of the samples.Results: When gels were compared, the difference was seen in the resolution of spots. Protein spots were fading from high- to low-molecular weight masses. Hence, advanced devices in comparison to spitting method resulted in much clearer protein spots which in turn prove the validation of devices.Conclusions: In this study, we concluded that protein extraction could be possible by both methods such as passive drooling method and through advanced saliva collection devices (Pure·SAL™ and RNAPro·SAL™).