Cariogenicity induced by commercial carbonated beverages in an experimental biofilm-caries model

Rodrigo A. Giacaman; Vanesa Pailahual; Natalia Díaz-Garrido

doi:10.4103/ejd.ejd_188_17

European Journal of Dentistry, Table of Contents

CC BY-NC-ND 4.0 · Eur J Dent 2018; 12(01): 027-035
DOI: 10.4103/ejd.ejd_188_17

Original Article

Dental Investigation Society

Cariogenicity induced by commercial carbonated beverages in an experimental biofilm-caries model

Rodrigo A. Giacaman

¹Department of Oral Rehabilitation, Cariology Unit, University of Talca, Talca, Chile

²Interdisciplinary Excellence Research Program on Healthy Aging (PIEI-ES), University of Talca, Talca, Chile

,

Vanesa Pailahual

¹Department of Oral Rehabilitation, Cariology Unit, University of Talca, Talca, Chile

,

Natalia Díaz-Garrido

¹Department of Oral Rehabilitation, Cariology Unit, University of Talca, Talca, Chile

› Author Affiliations

Abstract

ABSTRACT

Objectives Frequent consumption of sugars-containing carbonated beverages has been associated with caries, but the consequences on the dental biofilm remain unclear. The aim was to evaluate the effect of commercial carbonated beverages and their sugar-free version on enamel and dentine demineralization and on the cariogenic properties of Streptococcus mutans biofilms. Materials and Methods Biofilms of S. mutans UA159 were grown on enamel and dentin slabs and exposed 3 times/ day for 5 min, to a commercial cola or orange-flavored carbonated beverage or to their sugar-free version. Biofilms/slabs were recovered to assess biomass, viable microorganisms, protein content and polysaccharides. Demineralization was estimated by the variation of Knoop surface microhardness. Results Exposures to the biofilm with sugars-containing carbonated beverages resulted in similar biomass, viable microorganisms, proteins, and polysaccharides than sucrose (P < 0.05). The sugar-free cola and orange-flavored drink showed lower effect on the biofilm, as compared with sucrose or their sugared version (P < 0.05). All of the products tested, included the sugar-free, showed higher demineralization than the negative control (P < 0.05). Conclusions: Sugars-containing carbonated beverages enhance cariogenic activity of S. mutans biofilms, comparable with sucrose. Sugar-free carbonated beverages also have a high demineralizing potential, without affecting biofilm properties.

Key words:

Carbonated beverages - cariogenicity - dental caries - oral biofilm - soft drinks - Streptococcus mutans - sucrose

Full Text

References

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