Evaluation of intracanal acetazolamide in late reimplanted rat teeth

ABSTRACT

Objective: The aim of this study was to evaluate the use of acetazolamide combined with different agents as intracanal medication in late reimplanted rat teeth. Materials and Methods:In 100 Wistar rats, divided into 5 groups of 20, one of the following medications was used: Acetazolamide liquid (AL); AL with calcium hydroxide powder (ALHC); acetazolamide powder with AL; acetazolamide powder with physiological solution; and calcium hydroxide with physiological solution (control). At 30 and 60 days after reimplantation, the animals were sacrificed, tissues were processed, and cuts were stained with hematoxylin and eosin. An optical microscope was used to determine the following: percentage of inflammatory root resorption (RRI); percentage of substitute root resorption (RRS); and presence of ankylosis. The data obtained was submitted for statistical analysis. Results: Group ALHC had a significantly higher RRS than the control group at 60 days (P = 0.01). Group AL showed significantly less ankylosis than the other groups, including the control, at 30 days. AL showed results similar to those of the control group with respect to RRS. Conclusion:Acetazolamide has the potential to be an effective intracanal medication.

Key words:

Acetazolamide - calcium hydroxide - intracanal medication - resorption

• REFERENCES

• 1 Tronstad L. Root resorption – Etiology, terminology and clinical manifestations. Endod Dent Traumatol 1988; 4: 241-52
• 2 Andreasen JO, Borum MK, Jacobsen HL, Andreasen FM. Replantation of 400 avulsed permanent incisors 4. Factors related to periodontal ligament healing. Endod Dent Traumatol 1995; 11: 76-89
• 3 Babaji P, Melkundi M, Devanna R, Suresh BS, Chaurasia VR, Gopinath PV. et al. In vitro comparative evaluation of different storage media (hank's balanced salt solution, propolis, Aloe vera, and pomegranate juice) for preservation of avulsed tooth. Eur J Dent 2017; 11: 71-5
• 4 Andreasen JO, Andreasen FM, Andersson L. Textbook and Color Atlas of Traumatic Injuries to the Teeth. Oxford, UK ; Ames, Iowa: Blackwell Munksgaard; 2007
• 5 Mori GG, Garcia RB. Microscopic study of the effect of root surface treatment with acetazolamida in avulsed and reimplanted rat teeth. Rev Fac Odontol Bauru 2002; 10: 180-5
• 6 Mori GG, Garcia RB, Gomes de Moraes I. Morphometric and microscopic evaluation of the effect of solution of acetazolamide as an intracanal therapeutic agent in late reimplanted rat teeth. Dent Traumatol 2006; 22: 36-40
• 7 Mori GG, Moraes IG, Nunes DC, Castilho LR, Poi WR. Biocompatibility of acetazolamide pastes in the subcutaneous tissue of rats. Braz Dent J 2009; 20: 17-21
• 8 Mori GG, Poi WR, Castilho LR. Evaluation of the anti-resorptive ability of an experimental acetazolamide paste for the treatment of late replanted teeth: A study in rats. Dent Traumatol 2013; 29: 34-40
• 9 dos Santos CL, Sonoda CK, Poi WR, Panzarini SR, Sundefeld ML, Negri MR. et al. Delayed replantation of rat teeth after use of reconstituted powdered milk as a storage medium. Dent Traumatol 2009; 25: 51-7
• 10 Gregoriou AP, Jeansonne BG, Musselman RJ. Timing of calcium hydroxide therapy in the treatment of root resorption in replanted teeth in dogs. Endod Dent Traumatol 1994; 10: 268-75
• 11 Chen H, Teixeira FB, Ritter AL, Levin L, Trope M. The effect of intracanal anti-inflammatory medicaments on external root resorption of replanted dog teeth after extended extra-oral dry time. Dent Traumatol 2008; 24: 74-8
• 12 Fuss Z, Tsesis I, Lin S. Root resorption – Diagnosis, classification and treatment choices based on stimulation factors. Dent Traumatol 2003; 19: 175-82
• 13 Trope M, Moshonov J, Nissan R, Buxt P, Yesilsoy C. Short vs. long-term calcium hydroxide treatment of established inflammatory root resorption in replanted dog teeth. Endod Dent Traumatol 1995; 11: 124-8
• 14 Finucane D, Kinirons MJ. External inflammatory and replacement resorption of luxated, and avulsed replanted permanent incisors: A review and case presentation. Dent Traumatol 2003; 19: 170-4
• 15 Chappuis V, von Arx T. Replantation of 45 avulsed permanent teeth: A 1-year follow-up study. Dent Traumatol 2005; 21: 289-96
• 16 Ferreira MM, Botelho MF, Abrantes M, Carvalho L, Carrilho E. Histologic evaluation of the effect of mineral trioxide aggregate-fillapex as a root canal sealer in rat teeth submitted to late replantation. Eur J Dent 2017; 11: 89-93
• 17 Panzarini SR, Gulinelli JL, Saito CT, Poi WR, Sonoda CK, Américo de Oliveira J. et al. Short-term vws. long-term calcium hydroxide therapy after immediate tooth replantation: A histomorphometric study in monkey's teeth. Dent Traumatol 2012; 28: 226-32