Effect of adhesive mode and chlorhexidine on microtensile strength of universal bonding agent to sound and caries-affected dentins

Joyce Figueiredo Macedo de Lima; Danielle Wajngarten; Fariya Islam; Johanna Clifford; Ana Carolina Botta

doi:10.4103/ejd.ejd_239_18

European Journal of Dentistry, Inhaltsverzeichnis

CC BY-NC-ND 4.0 · Eur J Dent 2018; 12(04): 553-558
DOI: 10.4103/ejd.ejd_239_18

Original Article

Dental Investigation Society

Effect of adhesive mode and chlorhexidine on microtensile strength of universal bonding agent to sound and caries-affected dentins

Joyce Figueiredo Macedo de Lima

¹Department of Restorative Dentistry, Piracicaba School of Dentistry, Unicamp, Piracicaba, Sao Paulo, Brazil

,

Danielle Wajngarten

²Department of Social Dentistry, Araraquara Dental School, Sao Paulo State University (UNESP), Araraquara, Sao Paulo, Brazil

,

Fariya Islam

³Department of General Dentistry, Stony Brook University, School of Dental Medicine, NY, USA

,

Johanna Clifford

³Department of General Dentistry, Stony Brook University, School of Dental Medicine, NY, USA

,

Ana Carolina Botta

³Department of General Dentistry, Stony Brook University, School of Dental Medicine, NY, USA

› Institutsangaben

Abstract

ABSTRACT

Objective: The main objective of this study is to assess the effect of the adhesive mode and chlorhexidine (CHX) on the microtensile strength of a universal bonding agent to sound and caries-affected dentins. Materials and Methods: Six intact third molars and six decayed third molars were sectioned to obtain two middle dentin specimens per tooth. Prime and Bond Elect Universal (Dentsply) was applied to specimens in two different modes: Etch-and-rinse (ER, phosphoric acid for 15s) and self-etch (SE, no phosphoric acid). 2% CHX (Cavity Cleanser, Bisco) was applied to both dentins for 30 s. Specimens were randomly assigned into eight groups (n = 3) according to the treatments applied to sound and caries-affected dentins: SE; ER; SE + CHX; and ER + CHX. All specimens were restored with TPH Spectra High Viscosity (Dentsply) composite resin and sectioned to obtain specimens of 0.8 ± 0.1 mm². The microtensile bond strength test was conducted at 1 mm/min. The data were statistically analyzed by ANOVA and multiple comparisons' test (α = 0.05). Results: There was no significant difference between sound dentin and caries-affected dentin (P = 0.132). The highest bond strength was obtained with the application of the ER (31.81MPa). SE + CHX promoted the lowest bond strength with no statistical difference to ER + CHX (P > 0.05). Conclusions: ER mode for the universal bonding agent yielded the highest bond strength to sound and caries-affected dentins. CHX reduced the bond strength to both dentins regardless the application of phosphoric acid. Clinical Significance: The universal bonding agent increased the bond strength to sound and caries-affected dentins when applied by the ER mode.

Key words:

Acid etching - chlorhexidine - dentin - dentin adhesives - tensile strength

Volltext

Referenzen

REFERENCES
1 Van Meerbeek B, Yoshihara K, Yoshida Y, Mine A, De Munck J, Van Landuyt KL. et al. State of the art of self-etch adhesives. Dent Mater 2011; 27: 17-28
2 Pashley DH, Tay FR, Breschi L, Tjäderhane L, Carvalho RM, Carrilho M. et al. State of the art etch-and-rinse adhesives. Dent Mater 2011; 27: 1-6
3 De Munck J, Van Meerbeek B, Yoshida Y, Inoue S, Vargas M, Suzuki K. et al. Four-year water degradation of total-etch adhesives bonded to dentin. J Dent Res 2003; 82: 136-40
4 Hashimoto M, Ohno H, Sano H, Kaga M, Oguchi H. In vitro degradation of resin-dentin bonds analyzed by microtensile bond test, scanning and transmission electron microscopy. Biomaterials 2003; 24: 3795-803
5 Armstrong SR, Vargas MA, Chung I, Pashley DH, Campbell JA, Laffoon JE. et al. Resin-dentin interfacial ultrastructure and microtensile dentin bond strength after five-year water storage. Oper Dent 2004; 29: 705-12
6 Perdigão J. Dentin bonding as a function of dentin structure. Dent Clin North Am 2002; 46: 277-301 , vi
7 Mjör IA, Moorhead JE, Dahl JE. Reasons for replacement of restorations in permanent teeth in general dental practice. Int Dent J 2000; 50: 361-6
8 Mjör IA, Dahl JE, Moorhead JE. Age of restorations at replacement in permanent teeth in general dental practice. Acta Odontol Scand 2000; 58: 97-101
9 Pashley DH, Tay FR, Yiu C, Hashimoto M, Breschi L, Carvalho RM. et al. Collagen degradation by host-derived enzymes during aging. J Dent Res 2004; 83: 216-21
10 Hebling J, Pashley DH, Tjäderhane L, Tay FR. Chlorhexidine arrests subclinical degradation of dentin hybrid layers in vivo . J Dent Res 2005; 84: 741-6
11 Breschi L, Mazzoni A, Nato F, Carrilho M, Visintini E, Tjäderhane L. et al. Chlorhexidine stabilizes the adhesive interface: A 2-year in vitro study. Dent Mater 2010; 26: 320-5
12 Osorio R, Yamauti M, Osorio E, Ruiz-Requena ME, Pashley D, Tay F. et al. Effect of dentin etching and chlorhexidine application on metalloproteinase-mediated collagen degradation. Eur J Oral Sci 2011; 119: 79-85
13 Davis GE. Identification of an abundant latent 94-kDa gelatin-degrading metalloprotease in human saliva which is activated by acid exposure: Implications for a role in digestion of collagenous proteins. Arch Biochem Biophys 1991; 286: 551-4
14 Gunja-Smith Z, Woessner Jr JF. Activation of cartilage stromelysin-1 at acid pH and its relation to enzyme pH optimum and osteoarthritis. Agents Actions 1993; 40: 228-31
15 Zhou J, Tan J, Chen L, Li D, Tan Y. The incorporation of chlorhexidine in a two-step self-etching adhesive preserves dentin bond in vitro . J Dent 2009; 37: 807-12
16 Imazato S, Kinomoto Y, Tarumi H, Ebisu S, Tay FR. Antibacterial activity and bonding characteristics of an adhesive resin containing antibacterial monomer MDPB. Dent Mater 2003; 19: 313-9
17 Imazato S, Tay FR, Kaneshiro AV, Takahashi Y, Ebisu S. An in vivo evaluation of bonding ability of comprehensive antibacterial adhesive system incorporating MDPB. Dent Mater 2007; 23: 170-6
18 Osorio R, Erhardt MC, Pimenta LA, Osorio E, Toledano M. EDTA treatment improves resin-dentin bonds' resistance to degradation. J Dent Res 2005; 84: 736-40
19 Sauro S, Mannocci F, Toledano M, Osorio R, Pashley DH, Watson TF. et al. EDTA or H3PO4/NaOCl dentine treatments may increase hybrid layers' resistance to degradation: A microtensile bond strength and confocal-micropermeability study. J Dent 2009; 37: 279-88
20 Imbery TA, Kennedy M, Janus C, Moon PC. Evaluating EDTA as a substitute for phosphoric acid-etching of enamel and dentin. Gen Dent 2012; 60: e55-61
21 Sorsa T, Tjäderhane L, Konttinen YT, Lauhio A, Salo T, Lee HM. et al. Matrix metalloproteinases: Contribution to pathogenesis, diagnosis and treatment of periodontal inflammation. Ann Med 2006; 38: 306-21
22 Tjäderhane L, Nascimento FD, Breschi L, Mazzoni A, Tersariol IL, Geraldeli S. et al. Strategies to prevent hydrolytic degradation of the hybrid layer-A review. Dent Mater 2013; 29: 999-1011
23 Mobarak EH. Effect of chlorhexidine pretreatment on bond strength durability of caries-affected dentin over 2-year aging in artificial saliva and under simulated intrapulpal pressure. Oper Dent 2011; 36: 649-60
24 Sezinando A, Luque-Martinez I, Muñoz MA, Reis A, Loguercio AD, Perdigão J. et al. Influence of a hydrophobic resin coating on the immediate and 6-month dentin bonding of three universal adhesives. Dent Mater 2015; 31: e236-46
25 Young DA, Nový BB, Zeller GG, Hale R, Hart TC, Truelove EL. et al. The American Dental Association Caries Classification System for clinical practice: A report of the American Dental Association Council on scientific affairs. J Am Dent Assoc 2015; 146: 79-86
26 Pashley DH, Tao L, Boyd L, King GE, Horner JA. Scanning electron microscopy of the substructure of smear layers in human dentine. Arch Oral Biol 1988; 33: 265-70
27 Leitune VC, Portella FF, Bohn PV, Collares FM, Samuel SM. Influence of chlorhexidine application on longitudinal adhesive bond strength in deciduous teeth. Braz Oral Res 2011; 25: 388-92
28 Bertassoni LE, Habelitz S, Kinney JH, Marshall SJ, Marshall Jr GW. Biomechanical perspective on the remineralization of dentin. Caries Res 2009; 43: 70-7
29 Rosa WL, Piva E, Silva AF. Bond strength of universal adhesives: A systematic review and meta-analysis. J Dent 2015; 43: 765-76
30 Perdigão J. Dentin bonding-variables related to the clinical situation and the substrate treatment. Dent Mater 2010; 26: e24-37
31 Tosun G, Koyuturk AE, Sener Y, Sengun A. Bond strength of two total-etching bonding systems on caries-affected and sound primary teeth dentin. Int J Paediatr Dent 2008; 18: 62-9
32 Wagner A, Wendler M, Petschelt A, Belli R, Lohbauer U. Bonding performance of universal adhesives in different etching modes. J Dent 2014; 42: 800-7
33 Muñoz MA, Luque I, Hass V, Reis A, Loguercio AD, Bombarda NH. et al. Immediate bonding properties of universal adhesives to dentine. J Dent 2013; 41: 404-11
34 Tekçe N, Tuncer S, Demirci M, Balci S. Do matrix metalloproteinase inhibitors improve the bond durability of universal dental adhesives?. Scanning 2016; 38: 535-44
35 Tani C, Finger WJ. Effect of smear layer thickness on bond strength mediated by three all-in-one self-etching priming adhesives. J Adhes Dent 2002; 4: 283-9
36 De Munck J, Van Landuyt K, Peumans M, Poitevin A, Lambrechts P, Braem M. et al. A critical review of the durability of adhesion to tooth tissue: Methods and results. J Dent Res 2005; 84: 118-32
37 Nishitani Y, Yoshiyama M, Wadgaonkar B, Breschi L, Mannello F, Mazzoni A. et al. Activation of gelatinolytic/collagenolytic activity in dentin by self-etching adhesives. Eur J Oral Sci 2006; 114: 160-6
38 Tay FR, Pashley DH, Loushine RJ, Weller RN, Monticelli F, Osorio R. et al. Self-etching adhesives increase collagenolytic activity in radicular dentin. J Endod 2006; 32: 862-8
39 Liu Y, Tjäderhane L, Breschi L, Mazzoni A, Li N, Mao J. et al. Limitations in bonding to dentin and experimental strategies to prevent bond degradation. J Dent Res 2011; 90: 953-68
40 Boushell LW, Swift Jr EJ. Critical appraisal. Dentin bonding: Matrix metalloproteinases and chlorhexidine. J Esthet Restor Dent 2011; 23: 347-52
41 Campos EA, Correr GM, Leonardi DP, Pizzatto E, Morais EC. Influence of chlorhexidine concentration on microtensile bond strength of contemporary adhesive systems. Braz Oral Res 2009; 23: 340-5
42 Gunaydin Z, Yazici AR, Cehreli ZC. In vivo and in vitro effects of chlorhexidine pretreatment on immediate and aged dentin bond strengths. Oper Dent 2016; 41: 258-67
43 Di Hipólito V, Rodrigues FP, Piveta FB, Azevedo Lda C, Bruschi Alonso RC, Silikas N. et al. Effectiveness of self-adhesive luting cements in bonding to chlorhexidine-treated dentin. Dent Mater 2012; 28: 495-501
44 Blackburn RS, Harvey A, Kettle LL, Manian AP, Payne JD, Russell SJ. et al. Sorption of chlorhexidine on cellulose: Mechanism of binding and molecular recognition. J Phys Chem B 2007; 111: 8775-84
45 Kim J, Uchiyama T, Carrilho M, Agee KA, Mazzoni A, Breschi L. et al. Chlorhexidine binding to mineralized versus demineralized dentin powder. Dent Mater 2010; 26: 771-8