ABSTRACT
Objectives: The purpose of this study was to evaluate the cytotoxicity of a new bioceramic-based
root canal sealer (BioMM) by direct and indirect contact with human fibroblasts and
to compare it with a zinc oxide-eugenol sealer, the Pulp Canal Sealer-extended working
time (PCS-EWT). Materials and Methods: Cell viability was assessed through direct and indirect contact between human fibroblasts
and sealer. Direct contact was performed at 24 h, whereas the indirect contact was
performed at 24 and 48 h at different concentrations: 100%, 50%, and 25%. After direct
contact, 3-4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used
and the optical density was measured by a spectrophotometer. Giemsa stain was also
performed for a qualitative evaluation of the cells.Statistical Analysis Used: Shapiro–Wilk test was used to verify the normality of distribution of the variable.
Data were analyzed by analysis of variance and Tukey tests using SPSS for Windows
software 18.0. The significance level used was P < 0.05.Results: Direct contact showed a significantly higher cell viability with BioMM as compared
to PCS-EWT (P = 0.002). Cell viability at 24 h was significantly higher with BioMM compared to
PCS-EWT for the concentrations of 50% (P = 0.004) and 25% (P = 0.003), whereas no significant difference was noted at 100% (P = 0.141). Cell viability at 48 h was significantly higher with BioMM as compared
to PCS-EWT at 25% (P = 0.007). No significant difference was observed at 100% (P = 0.484) and 50% (P = 0.185).Conclusion: BioMM may be considered minimally cytotoxic if accidentally extruded into the periapical
tissues.
Key words:
4,5-dimethylthiazol-2-yl)-2 - 5-diphenyltetrazolium bromide - bioceramic - cell viability
- sealer
