Shifts of subgingival bacterial population after nonsurgical and pharmacological therapy of localized aggressive periodontitis, followed for 1 year by Ion Torrent PGM platform

Giuseppina Campisciano; Annamaria Toschetti; Manola Comar; Rosanna Di Taranto; Federico Berton; Claudio Stacchi

doi:10.4103/ejd.ejd_309_16

European Journal of Dentistry, Inhaltsverzeichnis

CC BY-NC-ND 4.0 · Eur J Dent 2017; 11(01): 126-129
DOI: 10.4103/ejd.ejd_309_16

Case Report

Dental Investigation Society

Shifts of subgingival bacterial population after nonsurgical and pharmacological therapy of localized aggressive periodontitis, followed for 1 year by Ion Torrent PGM platform

Giuseppina Campisciano

¹Department of Medical, Surgical and Health Sciences, University of Trieste, Trieste, Italy

²Advanced Diagnostics Department, Institute for Maternal and Child Health, IRCCS “Burlo Garofolo”, Trieste, Italy

,

Annamaria Toschetti

³Private Practice, Gorizia, Italy

,

Manola Comar

¹Department of Medical, Surgical and Health Sciences, University of Trieste, Trieste, Italy

²Advanced Diagnostics Department, Institute for Maternal and Child Health, IRCCS “Burlo Garofolo”, Trieste, Italy

,

Rosanna Di Taranto

³Private Practice, Gorizia, Italy

,

Federico Berton

¹Department of Medical, Surgical and Health Sciences, University of Trieste, Trieste, Italy

,

Claudio Stacchi

¹Department of Medical, Surgical and Health Sciences, University of Trieste, Trieste, Italy

› Institutsangaben

Abstract

ABSTRACT

The possibility of targeting the hypervariable region V3 of the 16S rRNA gene using Ion Torrent Personal Genome Machine (PGM) could provide a complete analysis of subgingival plaque samples, potentially able to identify microbiological species missed by culture-based methods. A 16-year-old female smoker patient, affected by localized aggressive periodontitis, underwent a full-mouth disinfection protocol and was inserted in a 3-month recall program. Microbiological samples were collected at baseline and at 30, 100, 365 days follow-up and analyzed by Ion Torrent PGM. Capnocytophaga, Fusobacterium, Prevotella, and Treponema were the most represented pathogens at baseline. Nonsurgical treatment and systemic antibiotics drastically lowered the anaerobic species, and their presence remained limited after 100 days, while a consistent recolonization by anaerobic bacteria was detected at 365 days. The patient showed a general improvement of periodontal conditions. Differently from polymerase chain reaction and other microarray techniques, Ion Torrent performs a quantitative analysis of the microbiota, irrespective of the searched species. An accurate definition of the shifts of the bacterial community might help periodontal researchers for a better understanding of the impact of different treatment approaches or in intercepting nonresponsive conditions.

Key words:

Anaerobic bacteria - full-mouth disinfection - Ion Torrent Personal Genome Machine - localized aggressive periodontitis - nonsurgical treatment

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Referenzen

REFERENCES
1 Armitage GC, Cullinan MP. Comparison of the clinical features of chronic and aggressive periodontitis. Periodontol 2000 2010; 53: 12-27
2 Armitage GC. Comparison of the microbiological features of chronic and aggressive periodontitis. Periodontol 2000 2010; 53: 70-88
3 Riep B, Edesi-Neuss L, Claessen F, Skarabis H, Ehmke B, Flemmig TF. et al. Are putative periodontal pathogens reliable diagnostic markers?. J Clin Microbiol 2009; 47: 1705-11
4 Lang NP, Bartold PM, Cullinan M, Jeffcoat M, Mombelli A, Murakami S. et al. Consensus report: Aggressive periodontitis. Ann Periodontol 1999; 4: 53
5 Nishihara T, Koseki T. Microbial etiology of periodontitis. Periodontol 2000 2004; 36: 14-26
6 Paster BJ, Boches SK, Galvin JL, Ericson RE, Lau CN, Levanos VA. et al. Bacterial diversity in human subgingival plaque. J Bacteriol 2001; 183: 3770-83
7 Saglie FR, Smith CT, Newman MG, Carranza Jr. FA, Pertuiset JH, Cheng L. et al. The presence of bacteria in the oral epithelium in periodontal disease. II. Immunohistochemical identification of bacteria. J Periodontol 1986; 57: 492-500
8 Christersson LA, Wikesjö UM, Albini B, Zambon JJ, Genco RJ. Tissue localization of Actinobacillus actinomycetemcomitans in human periodontitis. II. Correlation between immunofluorescence and culture techniques. J Periodontol 1987; 58: 540-5
9 He T, Hayashi J, Yamamoto M, Ishikawa I. Genotypic characterization of Actinobacillus actinomycetemcomitans isolated from periodontitis patients by arbitrarily primed polymerase chain reaction. J Periodontol 1998; 69: 69-75
10 Sanz M, Lau L, Herrera D, Morillo JM, Silva A. Methods of detection of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis and Tannerella forsythensis in periodontal microbiology, with special emphasis on advanced molecular techniques: A review. J Clin Periodontol 2004; 31: 1034-47
11 Germano F, Bramanti E, Arcuri C, Cecchetti F, Cicciù M. Atomic force microscopy of bacteria from periodontal subgingival biofilm: Preliminary study results. Eur J Dent 2013; 7: 152-8
12 Jünemann S, Prior K, Szczepanowski R, Harks I, Ehmke B, Goesmann A. et al. Bacterial community shift in treated periodontitis patients revealed by ion torrent 16S rRNA gene amplicon sequencing. PLoS One 2012; 7: e41606
13 Milani C, Hevia A, Foroni E, Duranti S, Turroni F, Lugli GA. et al. Assessing the fecal microbiota: An optimized ion torrent 16S rRNA gene-based analysis protocol. PLoS One 2013; 8: e68739
14 Parameter on aggressive periodontitis. American Academy of Periodontology. J Periodontol 2000; 71 (Suppl. 05) 867-9
15 Quirynen M, Bollen CM, Vandekerckhove BN, Dekeyser C, Papaioannou W, Eyssen H. Full-vs. partial-mouth disinfection in the treatment of periodontal infections: Short-term clinical and microbiological observations. J Dent Res 1995; 74: 1459-67
16 van Winkelhoff AJ, Rodenburg JP, Goené RJ, Abbas F, Winkel EG, de Graaff J. Metronidazole plus amoxycillin in the treatment of Actinobacillus actinomycetemcomitans associated periodontitis. J Clin Periodontol 1989; 16: 128-31
17 Akincibay H, Orsal SO, Sengün D, Tözüm TF. Systemic administration of doxycycline versus metronidazole plus amoxicillin in the treatment of localized aggressive periodontitis: A clinical and microbiologic study. Quintessence Int 2008; 39: e33-9
18 Lang NP, Tonetti MS. Periodontal risk assessment (PRA) for patients in supportive periodontal therapy (SPT). Oral Health Prev Dent 2003; 1: 7-16
19 Sundquist A, Bigdeli S, Jalili R, Druzin ML, Waller S, Pullen KM. et al. Bacterial flora-typing with targeted, chip-based pyrosequencing. BMC Microbiol 2007; 7: 108
20 Harper DS, Robinson PJ. Correlation of histometric, microbial, and clinical indicators of periodontal disease status before and after root planing. J Clin Periodontol 1987; 14: 190-6
21 Wade WG, Moran J, Morgan JR, Newcombe R, Addy M. The effects of antimicrobial acrylic strips on the subgingival microflora in chronic periodontitis. J Clin Periodontol 1992; 19: 127-34
22 Quirynen M, Mongardini C, de Soete M, Pauwels M, Coucke W, van Eldere J. et al. The rôle of chlorhexidine in the one-stage full-mouth disinfection treatment of patients with advanced adult periodontitis. Long-term clinical and microbiological observations. J Clin Periodontol 2000; 27: 578-89
23 Keestra JA, Grosjean I, Coucke W, Quirynen M, Teughels W. Non-surgical periodontal therapy with systemic antibiotics in patients with untreated aggressive periodontitis: A systematic review and meta-analysis. J Periodontal Res 2015; 50: 689-706
24 Lang NP, Tan WC, Krähenmann MA, Zwahlen M. A systematic review of the effects of full-mouth debridement with and without antiseptics in patients with chronic periodontitis. J Clin Periodontol 2008; 35 (Suppl. 08) 8-21
25 Eberhard J, Jervøe-Storm PM, Needleman I, Worthington H, Jepsen S. Full-mouth treatment concepts for chronic periodontitis: A systematic review. J Clin Periodontol 2008; 35: 591-604