Antimicrobial efficacy of 2.5% sodium hypochlorite, 2% chlorhexidine, and ozonated water as irrigants in mesiobuccal root canals with severe curvature of mandibular molars

Sergio Luiz Pinheiro; Caio Cesar da Silva; Lucas Augusto da Silva; Marina P. Cicotti; Carlos Eduardo da Silveira Bueno; Carlos Eduardo Fontana; Letícia R. Pagrion; Natália P. Dalmora; Thaís T. Daque; Francisco UF de Campos

doi:10.4103/ejd.ejd_324_17

European Journal of Dentistry, Inhaltsverzeichnis

CC BY-NC-ND 4.0 · Eur J Dent 2018; 12(01): 094-099
DOI: 10.4103/ejd.ejd_324_17

Original Article

Dental Investigation Society

Antimicrobial efficacy of 2.5% sodium hypochlorite, 2% chlorhexidine, and ozonated water as irrigants in mesiobuccal root canals with severe curvature of mandibular molars

Sergio Luiz Pinheiro

¹Department of Endodontics, Pontifícia Universidade Católica de Campinas, Campinas, SP, Brazil

,

Caio Cesar da Silva

¹Department of Endodontics, Pontifícia Universidade Católica de Campinas, Campinas, SP, Brazil

,

Lucas Augusto da Silva

¹Department of Endodontics, Pontifícia Universidade Católica de Campinas, Campinas, SP, Brazil

,

Marina P. Cicotti

¹Department of Endodontics, Pontifícia Universidade Católica de Campinas, Campinas, SP, Brazil

,

Carlos Eduardo da Silveira Bueno

¹Department of Endodontics, Pontifícia Universidade Católica de Campinas, Campinas, SP, Brazil

,

Carlos Eduardo Fontana

¹Department of Endodontics, Pontifícia Universidade Católica de Campinas, Campinas, SP, Brazil

,

Letícia R. Pagrion

¹Department of Endodontics, Pontifícia Universidade Católica de Campinas, Campinas, SP, Brazil

,

Natália P. Dalmora

¹Department of Endodontics, Pontifícia Universidade Católica de Campinas, Campinas, SP, Brazil

,

Thaís T. Daque

¹Department of Endodontics, Pontifícia Universidade Católica de Campinas, Campinas, SP, Brazil

,

Francisco UF de Campos

²Department of Endodontics, São Leopoldo Mandic, Campinas, SP, Brazil

› Institutsangaben

Abstract

ABSTRACT

Objective: The aim of this study is to evaluate the antimicrobial efficacy of 2.5% sodium hypochlorite, 2% chlorhexidine, and ozonated water on biofilms of Enterococcus faecalis, Streptococcus mutans, and Candida albicans in mesiobuccal root canals with severe curvature of mandibular molars. Materials and Methods: This was an experimental ex vivo study in microbiologic laboratory. Sixty mesiobuccal root canals with severe curvature of mandibular molars were contaminated with standard strains of E. faecalis, S. mutans, and C. albicans. The specimens were randomly divided into four groups (n = 15) according to irrigating solution: SH: 2.5% sodium hypochlorite; CH: 2% chlorhexidine; O₃: ozonated water; and control: double-distilled water. The mesiobuccal root canals of all groups were instrumented with the WaveOne Gold Primary reciprocating system. Three cycles of instrumentation with three short in-and-out brushing motions were performed: (1) in the coronal third, (2) in the middle third, and (3) in the apical third of the canal. A ProGlider file was used before the first cycle. Statistical Analysis: Statistical analysis was performed using one-way analysis of variance followed by Tukey’s multiple comparison test. Samples were collected for viable bacterial counts before and after instrumentation. Results: All groups showed significant biofilm reduction after irrigation (P < 0.01). After instrumentation, sodium hypochlorite (98.07%), chlorhexidine (98.31%), and ozonated water (98.02%) produced a significantly reduction in bacterial counts compared with double-distilled water (control, 72.98%) (P < 0.01). Conclusion: All irrigants tested in this study showed similar antimicrobial activity. Thus, ozonated water may be an option for microbial reduction in the root canal system.

Key words:

Endodontic treatment - irrigation - reciprocating system

Volltext

Referenzen

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