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CC BY-NC-ND 4.0 · Eur J Dent 2018; 12(02): 281-286
DOI: 10.4103/ejd.ejd_34_18
Original Article
European Journal of Dentistry

Effect of heat treatment on cytotoxicity of self-adhesive resin cements: Cell viability analysis

Celso Afonso Klein-Júnior
1   Department of Operative Dentistry, School of Dentistry, Lutheran University of Brazil, Cachoeira do Sul, Rio Grande do Sul, Brazil
,
Roberto Zimmer
1   Department of Operative Dentistry, School of Dentistry, Lutheran University of Brazil, Cachoeira do Sul, Rio Grande do Sul, Brazil
,
Guilherme Scotta Hentschke
2   Department of Cell Biology, Lutheran University of Brazil, Cachoeira do Sul, Rio Grande do Sul, Brazil
,
Denise Cantarelli Machado
3   Neuroscience Department, Brain Institute, Neuroscience Laboratory, Pontifical Catholic University of Rio Grande do Sul, Rio Grande do Sul, Brazil
,
Rubem Beraldo dos Santos
1   Department of Operative Dentistry, School of Dentistry, Lutheran University of Brazil, Cachoeira do Sul, Rio Grande do Sul, Brazil
,
Eduardo Galia Reston
1   Department of Operative Dentistry, School of Dentistry, Lutheran University of Brazil, Cachoeira do Sul, Rio Grande do Sul, Brazil
› Author Affiliations
Further Information

Publication History

Publication Date:
16 September 2019 (online)

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ABSTRACT

Objective: The aim of the study was to assess, in vitro, the influence on cytotoxicity of heat treatment applied before photopolymerization, while mixing three self-adhesive resin cements, in an NIH/3T3 fibroblast cell culture, based on cell viability measures. Methods: Samples were divided into three groups: (1) no heat treatment while mixing (control), (2) 37°C, and (3) 60°C heat treatment while mixing. Cements were light-cured immediately after mixing and immersed in Dulbecco's Modified Eagle Media for the extraction of possibly uncured products after 24 h and 7 days. Cultures contained 0.5 mL of NIH/3T3 fibroblasts per well at a concentration of 0.4 × 105 cells/mL and specific extracts for each sample. Statistical Analysis Used: Data were statistically analyzed with ANOVA and post hoc Student–Newman–Keuls (significance of 5%). Results: Cement cytotoxicity increased with time, as shown by the higher values observed at 7 days. There was a slight difference in intragroup cytotoxicity levels between 24 h and 7 days. Heat treatment at 60°C was associated with a major decrease in cytotoxicity levels in all three groups, both at 24 h and at 7 days, with no differences among the cements. Conclusions: Heat treatment at 60°C should be considered as a strategy to reduce cytotoxicity of self-adhesive resin cements, as evidenced by the results observed at 24 h and 7 days of analysis.

Key words:

Biocompatibility - dental materials - toxic substances
 

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