Antibacterial activity of ethanol extract of the leaves of Rosmarinus officinalis L. at different concentrations versus Streptococcus mutans: An In vitro comparative study

Edgar Janampa; Hugo Perez; Franco Mauricio; Daniel Alvítez-Temoche; Cesar Mauricio-Vilchez; Walter Gallo; Frank Mayta-Tovalino

doi:10.4103/ejgd.ejgd_148_20

European Journal of General Dentistry, Inhaltsverzeichnis

CC BY-NC-ND 4.0 · European Journal of General Dentistry 2020; 9(03): 152-156
DOI: 10.4103/ejgd.ejgd_148_20

Original Article

Antibacterial activity of ethanol extract of the leaves of Rosmarinus officinalis L. at different concentrations versus Streptococcus mutans: An In vitro comparative study

Edgar Janampa

¹Academic Department, Faculty of Dentistry, Universidad Nacional Federico Villarreal, Peru

,

Hugo Perez

¹Academic Department, Faculty of Dentistry, Universidad Nacional Federico Villarreal, Peru

,

Franco Mauricio

¹Academic Department, Faculty of Dentistry, Universidad Nacional Federico Villarreal, Peru

,

Daniel Alvítez-Temoche

¹Academic Department, Faculty of Dentistry, Universidad Nacional Federico Villarreal, Peru

,

Cesar Mauricio-Vilchez

¹Academic Department, Faculty of Dentistry, Universidad Nacional Federico Villarreal, Peru

,

Walter Gallo

²Social Responsibility Center, Faculty of Dentistry, Universidad Nacional Mayor de San Marcos, Peru

,

Frank Mayta-Tovalino

¹Academic Department, Faculty of Dentistry, Universidad Nacional Federico Villarreal, Peru

³Postgraduate Department, Faculty of Health Sciences, Universidad Científica del Sur, Peru

› Institutsangaben

Abstract

Abstract>

Aim: The study aimed to compare the in vitro antibacterial activity of an ethanol extract of Rosmarinus officinalis “romero” (EERO) at different concentrations with Streptococcus mutans ATCC 25175. Materials and Methods: We worked with three concentrations of 25%, 50%, and 75% of the EERO and compared these with the positive control chlorhexidine 0.12% and distilled water as a negative control. Seeding was performed in Müller–Hinton agar medium. The inhibitory effectiveness of EERO was determined using the disc diffusion method with the experimental solutions. The seeded and inoculated plates were incubated at 37°C, for 24 and 48 h. The reading was carried out according to the Kirby–Bauer method by measuring the diameter (mm) of the inhibition halo formed by the bacteria using a Vernier caliper and recorded on a data collection sheet. Results: In the 24-h group, chlorhexidine 0.12% showed the highest inhibition halos with a mean of 12.8 ± 0.8 mm, followed by EERO 75% with a similar mean of 12.1 ± 0.6 mm. Similarly, at 48 h, chlorhexidine 0.12% had the highest antibacterial activity of 13.0 ± 0.7 mm followed by EERO 75% with 12.2 ± 0.5 mm. Conclusions: The EERO showed inhibitory effectiveness against S. mutans, with the concentration of 75 mg/ml showing similar results, albeit not statistically significantly different, to those of chlorhexidine.

Keywords

Antibacterial activity - in vitro - Romero - Rosmarinus officinalis

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Referenzen

References
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