Dentinogenic differentiation potential of fast set white portland cements of a different origin on dental pulp stem cells

Hany Mohamed Aly Ahmed; Norhayati Luddin; Thirumulu Ponnuraj Kannan; Khairani Idah Mokhtar; Azlina Ahmad

doi:10.4103/ejgd.ejgd_42_17

European Journal of General Dentistry, Table of Contents

CC BY-NC-ND 4.0 · European Journal of General Dentistry 2017; 6(03): 115-122
DOI: 10.4103/ejgd.ejgd_42_17

Original Article

Dentinogenic differentiation potential of fast set white portland cements of a different origin on dental pulp stem cells

Hany Mohamed Aly Ahmed

Department of Restorative Dentistry, Faculty of Dentistry, 50603, Kuala Lumpur, Malaysia

,

Norhayati Luddin

Department of Restorative Dentistry, Faculty of Dentistry, 50603, Kuala Lumpur, Malaysia

,

Thirumulu Ponnuraj Kannan

Department of Restorative Dentistry, Faculty of Dentistry, 50603, Kuala Lumpur, Malaysia

¹Human Genome Centre, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, 16150, Kelantan, Malaysia

,

Khairani Idah Mokhtar

Department of Restorative Dentistry, Faculty of Dentistry, 50603, Kuala Lumpur, Malaysia

²Department of Fundamental Dental and Medical Science Department of Fundamental Dental and Medical Science, Kulliyyah of Dentistry, International Islamic University Malaysia, Bandar Indera Mahkota, 25200 Kuantan, Pahang, Malaysia

,

Azlina Ahmad

Department of Restorative Dentistry, Faculty of Dentistry, 50603, Kuala Lumpur, Malaysia

¹Human Genome Centre, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, 16150, Kelantan, Malaysia

› Author Affiliations

Abstract

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Abstract

Background: Advances in endodontic biomaterials are at the forefront of endodontic research. Purpose: This study aimed to compare the dentinogenic differentiation potential of extracts of two formulations (normal and fast set [FS] by the addition of calcium chloride dihydrate (CaCl₂.2H₂O) of white Portland cements (WPCs) of a different origin (Aalborg, Malaysia, and Egypt) on dental pulp stem cells (DPSCs). Materials and Methods: The material extracts at 12.5 mg/ml were applied on DPSCs cultured in 96-well plates. After 1, 3, 7 and 14 days of incubation, the RNA was extracted, cDNA was prepared, and the expression of four dentinogenic gene markers (bone gamma-carboxyglutamate protein, dentin sialophosphoprotein, runt-related transcription factor 2, and secreted phosphoprotein 1 [SPP1]) was examined using the real-time polymerase chain reaction. One-way analysis of variance was used for statistical analysis, and the level of significance was set at 0.05 (P = 0.05). Results: Significant differences were observed between Malaysian WPC (MAWPC) and Egyptian WPC (EGWPC) and FS MAWPC), FS EGWPC in 7 out of 15 and 6 out of 10 comparisons, respectively. While more expressions in EGWPC group were observed in four comparisons and three for MAWPC, all FS formulations showed higher expressions for FS MAWPC compared to FS EGWPC (P < 0.05). The addition of CaCl₂.2H₂O to MAWPC and EGWPC increased the upregulation of SPP1 gene at all-day intervals, which was not observed with other genes. Conclusions: The dentinogenic differentiation of DPSCs shows different expression profiles to extracts of normal and fast formulations of WPC. Extracts of WPC of different origin have different dentinogenic differentiation potential on DPSCs.

Keywords

Calcium chloride dihydrate - dentinogenic differentiation - Egypt - Malaysia - real-time polymerase chain reaction - white Portland cement

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References

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