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CC BY-NC-ND 4.0 · European Journal of General Dentistry 2019; 8(03): 71-75
DOI: 10.4103/ejgd.ejgd_57_19
Original Article

Influence of blood contamination and decontamination procedures on bond strength of a two-step etch and rinse adhesive system

Rafael Guerra Lund
1   Graduate Program in Dentistry, Department of Restorative Dentistry, School of Dentistry, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil
,
Isadora Augusta Da Silveira
1   Graduate Program in Dentistry, Department of Restorative Dentistry, School of Dentistry, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil
,
Juliana Silva Ribeiro
1   Graduate Program in Dentistry, Department of Restorative Dentistry, School of Dentistry, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil
,
Dayane Rubin
1   Graduate Program in Dentistry, Department of Restorative Dentistry, School of Dentistry, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil
,
Sonia Luque Peralta
2   Department of Restorative Dentistry, Metropolitan Faculty of Grande Fortaleza, Fortaleza, Ceará, Brazil
,
Carlos Enrique Cuevas-Suárez
1   Graduate Program in Dentistry, Department of Restorative Dentistry, School of Dentistry, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil
3   Department of Dental Materials Laboratory, Academic Area of Dentistry, Autonomous University of Hidalgo State, Pachuca, Hidalgo, México, México
,
Evandro Piva
1   Graduate Program in Dentistry, Department of Restorative Dentistry, School of Dentistry, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil
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Abstract

Purpose: This study evaluated the effect of blood contamination and decontamination procedures performed at different stages of bonding, on the microtensile bond strength (μTBS) of an etch-and-rinse adhesive to dentin. Materials and Methods: Standardized cylindrical cavities were prepared in bovine incisors and were randomly divided into five groups, each one was treated using these experimental conditions – Control: etching-rinsing/bonding without blood contamination; Group 1: etching-rising/blood contamination/decontamination/bonding; Group 2: etching-rising/bonding/blood contamination/decontamination; Group 3: etching-rising/bonding/blood contamination/decontamination/etching-rising/bonding; and Group 4: etching-rising/blood contamination/decontamination/etching-rising/bonding. Specimens were prepared for μTBS and were evaluated both immediately and after 6 months of storage in distilled water. Results: Blood contamination significantly reduced the μTBS (P < 0.001). Groups 2 and 3 showed the lowest μTBS values, both for 24 h and 6 month aging. Conclusions: Blood contamination tested at any of the different stages of the bonding procedure showed a negative effect on the μTBS. Recovering adhesion of blood-contaminated dentin did not depend only on cleaning with distilled water.

Keywords

Contaminants - decontamination - dentin-bonding agents - microtensile - resin composite

Financial support and sponsorship

Nil.




Publication History

Article published online:
01 November 2021

© 2019. European Journal of General Dentistry. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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