Enterococcus faecalis-Induced Nuclear Factor-Kappa Beta and Osteocalcin Expressions in Rat's Periapical Tissue Damage

Abstract

Background/Aims: Nuclear factor kappa beta (NF-κB) is a member of the transcription factor family, and it plays a key role in coordinating the expression of genes in many chronic inflammatory diseases. The main etiology of endodontic treatment failure is caused by bacteria in root canal, including Enterococcus faecalis. The aim of this study to determine NF-κβ and osteocalcin expressions during the periapical tissue damage due to induction of E. faecalis. Materials and Methods: Fifty-four male rats were randomly divided into two main groups, each of which had three subgroups. In Group C (control), every tooth was induced only by sterile brain–heart infusion broth (BHIb). Group C had three subgroups, namely Group C3 with 3 days inductionprocess, Group C10 with 10 days induction process, and Group C21 with 21 days induction process. In Group T, every tooth was induced by 10 μl of BHI-b E. faecalis ATCC212 (10⁶ CFU). Similarly, Group T also had three subgroups, namely Group T3 with 3 days induction process, Group T10 with 10 days induction process, and Group T21 with 21 days induction process. The animals were sacrificed based on their group schedule and then prepared for histological and immunohistochemical examinations of periapical tissue. Afterward, the expressions of NF-κβ and osteocalcin were calculated on the light microscope. Results: The results revealed that the number of cells expressed of NF-κβ and osteocalcin increased significantly in all sub-groups of Group B induced by E. faecalis compared to the control group. Conclusion: It can be concluded that the number of cells expressed NF-κβ and osteocalcin increase during the periapical tissue damage induced by E. faecalis.

Keywords

Enterococcus faecalis - nuclear factor kappa beta - osteocalsin - periapical - tissue damage

Financial support and sponsorship

Nil.

Publication History

Article published online:
01 November 2021

• References

• 1 Rôças IN, Jung IY, Lee CY, Siqueira JF Jr. Polymerase chain reaction identification of microorganisms in previously root-filled teeth in a South Korean population. J Endod 2004;30:504-8.
• 2 Pinheiro ET, Gomes BP, Ferraz CC, Sousa EL, Teixeira FB, Souza-Filho FJ. Microorganisms from canals of root-filled teeth with periapical lesions. Int Endod J 2003;36:1-1.
• 3 Hancock HH 3rd, Sigurdsson A, Trope M, Moiseiwitsch J. Bacteria isolated after unsuccessful endodontic treatment in a North American population. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2001;91:579-86.
• 4 Wang S, Deng Z, Seneviratne CJ, Cheung GS, Jin L, Zhao B, et al. Enterococcus faecalis promotes osteoclastogenesis and semaphorin 4D expression. Innate Immun 2015;21:726-35.
• 5 Liu T, Zhang L, Joo D, Sun SC. NF-κB signaling in inflammation. Signal Transduct Target Ther 2017;2.
• 6 Boyce BF, Yao Z, Xing L. Functions of nuclear factor-κB in bone. Ann N Y Acad Sci J Endod 2007;33:432-6.
• 7 Aubin JE, Bonnelye E. Osteoprotegerin and its ligand: A new paradigm for regulation of osteoclastogenesis and bone resorption. Osteoporos Int 2000;11:905-13.
• 8 Dumitrescu AL. Depression and inflammatory periodontal disease considerations-an interdisciplinary approach. Front Psychol 2016;7:347.
• 9 Rocas IN, Siqueira JF Jr., Santos KR. Association of Enterococcus faecalis with different forms of periradicular diseases. J Endod 2004;30:315-20.
• 10 Lorenzo J, Horowitz M, Choi Y. Osteoimmunology: Interactions of the bone and immune system. Endocr Rev 2008;29:403-40.
• 11 Park OJ, Kim J, Yang J, Yun CH, Han SH. Enterococcus faecalis inhibits osteoblast differentiation and induces chemokine expression. J Endod 2015;41:1480-5.
• 12 Tian Y, Zhang X, Zhang K, Song Z, Wang R, Huang S, et al. Effect of Enterococcus faecalis lipoteichoic acid on apoptosis in human osteoblast-like cells. J Endod 2013;39:632-7.