Correlation of Gleason Grading and Prognostic Immunohistochemistry Markers (Human Epidermal Growth Factor Receptor 2/neu and Androgen Receptor) in Prostatic Core Needle Biopsy: A Study in a Tertiary Care Center

Utsha Senapati; Moumita Sengupta; Chhanda Datta; Uttara Chatterjee; Dilip Kumar Pal; Diya Das; Piyali Poddar

doi:10.4103/ijmpo.ijmpo_139_17

Indian Journal of Medical and Paediatric Oncology, Table of Contents

CC BY-NC-ND 4.0 · Indian J Med Paediatr Oncol 2019; 40(02): 201-207
DOI: 10.4103/ijmpo.ijmpo_139_17

Original Article

Correlation of Gleason Grading and Prognostic Immunohistochemistry Markers (Human Epidermal Growth Factor Receptor 2/neu and Androgen Receptor) in Prostatic Core Needle Biopsy: A Study in a Tertiary Care Center

Authors

Utsha Senapati

Departments of Pathology, Institute of Post Graduate Medical Education and Research, Kolkata, West Bengal, India
Moumita Sengupta

Departments of Pathology, Institute of Post Graduate Medical Education and Research, Kolkata, West Bengal, India
Chhanda Datta

Departments of Pathology, Institute of Post Graduate Medical Education and Research, Kolkata, West Bengal, India
Uttara Chatterjee

Departments of Pathology, Institute of Post Graduate Medical Education and Research, Kolkata, West Bengal, India
Dilip Kumar Pal

Urosurgery, Institute of Post Graduate Medical Education and Research, Kolkata, West Bengal, India
Diya Das

Departments of Pathology, Institute of Post Graduate Medical Education and Research, Kolkata, West Bengal, India
Piyali Poddar

Urosurgery, Institute of Post Graduate Medical Education and Research, Kolkata, West Bengal, India

Abstract

Background: Worldwide prostate cancer is the most common cause of cancer and the second leading cause of cancer death among men. Transrectal ultrasound-guided core needle biopsies are the diagnostic modalities which help in proper categorization and grading of prostatic carcinoma, thus facilitating individualized treatment. These biopsies are the primary source for performance of additional diagnostic immunohistochemical testing for basal cell-associated markers to rule out the morphological mimicker and prognostic markers such as androgen receptor (AR) and human epidermal growth factor receptor 2 (HER2)/neu. Materials and Methods: A prospective, observational study was conducted in the department of pathology in collaboration with department of urosurgery of a tertiary care hospital. One hundred and nineteen patients diagnosed with prostatic nodules were included in this study. Values of serum prostate-specific antigen were recorded. Tissue for histopathological study was obtained in the form of core needle biopsy, and Gleason grade was calculated in all malignant cases. Immunohistochemistry for p63 and alpha-methylacyl-CoA racemase was performed as an additional test in premalignant cases. Prognostication of the prostate cancer cases was done using AR and HER2/neu. Results: A total of 119 cases aged between 20 and 90 years were included in this study. Malignant lesions reveal an increase in the percentage of AR staining in comparison to the benign glandular structure. The Gleason score with higher value (8–9) showed increased expression of HER2/neu receptor. Conclusion: Critical histopathological analysis of core needle biopsy along with immunohistochemical evaluation maximizes the diagnostic accuracy in prostate cancer cases and also helps in prognosis assessment.

Keywords

Androgen receptor - human epidermal growth factor receptor 2/neu - prostatic cancer - serum prostatic-specific antigen - transrectal ultrasound-guided core needle biopsies

Full Text

References

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