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CC BY-NC-ND 4.0 · Indian J Plast Surg 2018; 51(02): 177-181
DOI: 10.4103/ijps.IJPS_150_17
Original Article
Association of Plastic Surgeons of India

Adipose tissue: A natural resource for multipotent mesenchymal stem cells with potential translation to trigerminal layers

Bhaskar Vyas
Samanvaya Trust, Vadodara, Gujarat, India
,
Atul Shah
Samanvaya Trust, Vadodara, Gujarat, India
,
Anant Marathe
Samanvaya Trust, Vadodara, Gujarat, India
,
Ansarullah,
Rajni Vyas
Samanvaya Trust, Vadodara, Gujarat, India
,
Ramesh Bhonde
1   Manipal Institute of Regenerative Medicine, Bengaluru, Karnataka, India
› Author Affiliations
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Publication History

Publication Date:
26 July 2019 (online)

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ABSTRACT

Background: The article reports basic science research that establishes that adipose tissue (AT)-derived mesenchymal stem cells (MSCs) have a potential to transgerminal translation. Study Design: MSC confirmation was obtained by phenotypic spindle-shaped cells as well as with four positive and three negative markers. The translineage translation of adipose-derived MSCs (ADMSCs) was established. Materials and Methods: The lipoaspirate was subjected to enzymatic digestion with collagenase. Stromal vascular factor (SVF) was isolated. With two passages, pure culture of ADMSCs was obtained. They were translated to all the three germinal layers. Results: AT-derived SVF contains ~30% MSCs. They are capable of being translated into endoderm, mesoderm and ectoderm. Conclusion: AT is a rich source for MSCs, with immense research possibilities for regeneration and rejuvenation.

KEY WORDS

Adipose tissue - mesenchymal stem cells - stromal vascular fraction
 

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