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CC BY-NC-ND 4.0 · South Asian J Cancer 2018; 07(04): 236-239
DOI: 10.4103/sajc.sajc_118_17
ORIGINAL ARTICLE: Gynaecologic Cancers

Expression of p16INK4a and human papillomavirus 16 with associated risk factors in cervical premalignant and malignant lesions

Autor*innen

  • Abha Pandey

    Department of Pathology, Himalaya Institute of Medical Sciences, Swami Rama Himalayan University, Dehradun, Uttarakhand

  • Smita Chandra

    Department of Pathology, Himalaya Institute of Medical Sciences, Swami Rama Himalayan University, Dehradun, Uttarakhand

  • Ruchira Nautiyal

    Department of Obstetrics and Gynaecology, Himalaya Institute of Medical Sciences, Swami Rama Himalayan University, Dehradun, Uttarakhand, India

  • Vikas Shrivastav

    Department of Pathology, Himalaya Institute of Medical Sciences, Swami Rama Himalayan University, Dehradun, Uttarakhand

Financial support and sponsorship Nil.
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Abstract

Introduction: Human papilloma virus (HPV) which is causative factor for cervical cancer may interact with p16 leading to malignant transformation of cervical epithelial cells. The present study was conducted to assess the immunoexpression of p16 INK4a in premalignant and malignant lesions of cervix and to correlate it with HPV 16 expression. It was also intended to study the various risk factors which may be associated with cervical cancer in this north Himalayan region of India. Material and Methods: The study included 50 cases of premalignant and malignant cervical lesions and 50 controls diagnosed on histopathology over a period of one year. All the relevant clinical details were noted and both cases and controls were subjected to HPV 16 and p16 INK4a immunohistochemical staining. Results: 67% of subjects (including cases and controls) and 94% of the cases were positive for HPV 16 expression. p16 INK4a expression was negative in all the controls, positive in 96% of invasive cancer, 66.6% in HSIL and 37.5% in LSIL. Conclusion: Cervical cancer is associated with low socio economic status, illiteracy, smoking, early age of marriage and conception in north Himalayan region of India. HPV 16 infection is positive in both cases and controls indicating high prevalence of HPV 16 in this region. Neoplastic transformation by HPV is identified by over expression of p16 INK4a in premalignant and malignant cases. The immunopositivity of p16 INK4a increases with the severity of cervical lesions and thus may play an important role in stratification of premalignant and malignant lesions.

Key words

Cervical cancer - human papillomavirus 16 - p16INK4a - premalignant lesions


Publikationsverlauf

Artikel online veröffentlicht:
21. Dezember 2020

© 2018. MedIntel Services Pvt Ltd. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/.)

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  • References

  • 1 Ferlay J, Soerjomataram I, Ervik M, Dikshit R, Eser S, Mathers C, et al. Globocan. Vol. 10. Cancer Incidence and Mortality Worldwide: IARC Cancer Base. Lyon, France: International Agency for Research on Cancer; 2013. Available from: http://www.globocan.iarc.fr. [Last accessed on 2017 Jun 02].
  • 2 WHO. WHO guidelines for Screening and Treatment of Precancerous Lesions for Cervical Cancer Prevention. Geneva, Switzerland: World Health Organization; 2013.
  • 3 Kim SM, Lee JU, Lee DW, Kim MJ, Lee HN. The prognostic significance of p16, Ki-67, p63 and CK17 expression determined by immunohistochemical staining in cervical intraepithelial neoplasia. Korean J Obstet Gynecol 2011;54:184-91.
  • 4 Lambert AP, Anschau F, Schmitt VM. P16 INK4a expression in cervical premalignant and malignant lesions. Exp Mol Pathol 2006;80:192-6.
  • 5 Singh V, Husain N, Akhtar N, Khan MY, Sonkar AA, Kumar V, et al. P16 and p53 in HPV-positive versus HPV-negative oral squamous cell carcinoma: Do pathways differ? J Oral Pathol Med 2017;46:744-51.
  • 6 Dahiya N, Bachani D, Acharya AS, Sharma DN, Gupta S, Haresh KP, et al. Socio-demographic, reproductive and clinical profile of women diagnosed with advanced cervical cancer in a tertiary care institute of Delhi. J Obstet Gynaecol India 2017;67:53-60.
  • 7 Tavassoli FA, Devilee P. Pathology and genetics of tumours of the breast and female genital organs. In: World Health Organization Classification of Tumours. Lyon: IARC Press; 2003.
  • 8 Thakur A, Gupta B, Gupta A, Chauhan R. Risk factors for cancer cervix among rural women of a hilly state: A case-control study. Indian J Public Health 2015;59:45-8.
  • 9 dos Santos Silva I, Beral V. Socioeconomic differences in reproductive behaviour. IARC Sci Publ 1997;138:285-308.
  • 10 Eze JN, Emeka-Irem EN, Edegbe FO. A six-year study of the clinical presentation of cervical cancer and the management challenges encountered at a state teaching hospital in Southeast Nigeria. Clin Med Insights Oncol 2013;7:151-8.
  • 11 Bayo S, Bosch FX, de Sanjosé S, Muñoz N, Combita AL, Coursaget P, et al. Risk factors of invasive cervical cancer in Mali. Int J Epidemiol 2002;31:202-9.
  • 12 Thulaseedharan JV, Malila N, Hakama M, Esmy PO, Cheriyan M, Swaminathan R, et al. Socio demographic and reproductive risk factors for cervical cancer - a large prospective cohort study from rural India. Asian Pac J Cancer Prev 2012;13:2991-5.
  • 13 Shi N, Lu Q, Zhang J, Li L, Zhang J, Zhang F, et al. Analysis of risk factors for persistent infection of asymptomatic women with high-risk human papilloma virus. Hum Vaccin Immunother 2017;13:1-7.
  • 14 Biswas LN, Manna B, Maiti PK, Sengupta S. Sexual risk factors for cervical cancer among rural Indian women: A case-control study. Int J Epidemiol 1997;26:491-5.
  • 15 Hellman K, Lindquist D, Ranhem C, Wilander E, Andersson S. Human papillomavirus, p16(INK4A), and ki-67 in relation to clinicopathological variables and survival in primary carcinoma of the vagina. Br J Cancer 2014;110:1561-70.
  • 16 Bergeron C, Ronco G, Reuschenbach M, Wentzensen N, Arbyn M, Stoler M, et al. The clinical impact of using p16(INK4a) immunochemistry in cervical histopathology and cytology: An update of recent developments. Int J Cancer 2015;136:2741-51.
  • 17 Kanthiya K, Khunnarong J, Tangjitgamol S, Puripat N, Tanvanich S. Expression of the p16 and ki67 in cervical squamous intraepithelial lesions and cancer. Asian Pac J Cancer Prev 2016;17:3201-6.
  • 18 Cheah PL, Koh CC, Nazarina AR, Teoh KH, Looi LM. Correlation of p16INK4a immunoexpression and human papillomavirus (HPV) detected by in-situ hybridization in cervical squamous neoplasia. Malays J Pathol 2016;38:33-8.
  • 19 Queiroz C, Silva TC, Alves VA, Villa LL, Costa MC, Travassos AG, et al. P16(INK4a) expression as a potential prognostic marker in cervical pre-neoplastic and neoplastic lesions. Pathol Res Pract 2006;202:77-83.