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Open Access
CC BY-NC-ND 4.0 · Journal of Morphological Sciences 2017; 34(03): 203-206
DOI: 10.4322/jms.092815
Short Communication
Thieme Revinter Publicações Ltda Rio de Janeiro, Brazil

A modified automated protocol for EBER in situ hybridization staining of xenograft tissue

Authors

  • N. A. Aziz

    1   Molecular Pathology Unit, Cancer Research Centre - CaRC, Institute for Medical Research - IMR, Jalan Pahang, 50588, Kuala Lumpur, Malaysia

  • M. Ahmad

    1   Molecular Pathology Unit, Cancer Research Centre - CaRC, Institute for Medical Research - IMR, Jalan Pahang, 50588, Kuala Lumpur, Malaysia

  • A. Soo-Beng Khoo

    1   Molecular Pathology Unit, Cancer Research Centre - CaRC, Institute for Medical Research - IMR, Jalan Pahang, 50588, Kuala Lumpur, Malaysia
Further Information

Publication History

25 August 2015

22 August 2017

Publication Date:
18 October 2018 (online)

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Abstract

Introduction: Epstein-Barr virus encoded RNA (EBER) in situ hybridization (ISH) is a method for the identification of Epstein-Barr virus (EBV) infection in tissue sections. In our experience, the currently available automated EBER ISH staining protocol using the Bond-Max immunostainer is not suitable for xenograft tissue sample due to high background staining which impairs the interpretation of the staining. Methods: Here we describe a modified automated EBER ISH staining protocol for xenografts which involves the substitution of reagents for detecting the EBER probe. Results: This modified protocol significantly improved the quality and speed of the staining. Conclusion: The method described here will be useful for EBER ISH staining of xenograft tissue samples.

Keywords

Epstein-Barr virus - EBER - in situ hybridization - xenograft