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CC BY 4.0 · Brazilian Journal of Oncology 2019; 15: e-20190011
DOI: 10.5935/2526-8732.20190011
Case Report

Expression of EGFR family and p53 in a patient with Synchronous Primary Endometrial Adenocarcinoma and Clear Cell Renal Carcinoma: a Case Report

Expressão da família EGFR e p53 em paciente com Adenocarcinoma Endometrial e Carcinoma Renal de Células Claras primários e sincrônicos: Relato de Caso

Priscila Fernanda da Silva Martins
1   Centro Universitário UNA, Instituto de Ciências Biológicas e da Saúde - Belo Horizonte - Minas Gerais - Brazil
,
Izabella Cristina Alves Souza
1   Centro Universitário UNA, Instituto de Ciências Biológicas e da Saúde - Belo Horizonte - Minas Gerais - Brazil
,
Enio Ferreira
2   Universidade Federal de Minas Gerais, Departamento de Patologia - Belo Horizonte - Minas Gerais - Brazil
,
Emerson Soares Veloso
2   Universidade Federal de Minas Gerais, Departamento de Patologia - Belo Horizonte - Minas Gerais - Brazil
,
Tatiany L Silveira
2   Universidade Federal de Minas Gerais, Departamento de Patologia - Belo Horizonte - Minas Gerais - Brazil
,
Adam Underwood
3   Walsh University, Division of Math and Science - North Canton - Ohio - United States
,
Fabiano Conde Araujo
4   Faculdade de Medicina, Faminas-BH, Departamento de Medicina - Belo Horizonte - Minas Gerais - Brazil
,
Helen Lima Del-Puerto
2   Universidade Federal de Minas Gerais, Departamento de Patologia - Belo Horizonte - Minas Gerais - Brazil
› Author Affiliations Financial support: CNPq and Fapemig.
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ABSTRACT

We present a case of a 69 year-old, non-obese, post-menopausal woman diagnosed with synchronous endometrial adenocarcinoma and renal cell carcinoma. Immunolocalization and expression of the EGFR, HER-2, HER-3, HER-4 and p53 proteins by immunohistochemistry (IHC) was performed to investigate synchronous expression of these proteins. The endometrial adenocarcinoma was a well-differentiated tumor, with discernable glandular structure with moderate mitotic index. IHC revealed weak HER-2, HER-3 and HER-4 staining of cell membranes in well-differentiated areas, and intense HER-2 and p53 staining in papillary areas. The renal cell carcinoma showed clear cell type, solid tumor, Fuhrman nuclear grade 2, presence of necrosis and hemorrhage. IHC revealed intense HER-2 and HER-4 staining of cell membranes, weak EGFR staining, and negative expression for HER3 and p53 protein. In summary, overexpression of HER-2 and HER-4 may correlate with histological grade of the tumor and patient prognosis.

RESUMO

Apresentamos um relato de caso de uma paciente com 69 anos, não-obesa, pós-menopausa, diagnosticada com adenocarcinoma endometrial simultâneo ao carcinoma de células renais. A imunolocalização e expressão das proteínas EGFR, HER-2, HER-3, HER-4 e p53 através de imunohistoquímica (IHC) foi realizada para investigar a simultaneidade na expressão dessas proteínas em ambos tumores. O adenocarcinoma endometrial era um tumor bem diferenciado, com estruturas grandulares discerníveis e com índice mitótico moderado. A IHC revelou fraca marcação das proteínas HER-2, HER-3 e HER-4 nas membranas celulares em áreas bem diferenciadas, e marcação intensa de HER-2 e p53 em áreas papilares. O carcinoma de células renais apresentou tipo células clara, tumor sólido, grau 2 de malignidade nuclear, presença de necrose e hemorragia. IHC revelou marcação intensa de HER-2 e HER-4 nas membranas celulares, fraca de EGFR e negativa para HER-3 e p53. Em resumo, a super expressão das proteínas HER-2 e HER-4 podem estar correlacionadas ao grau histológico dos tumores e ao prognóstico da paciente.

Keywords:

Endometrial carcinoma - Renal clear cell carcinoma - EGFR family - p53

Descritores:

Carcinoma Endometrial - Carcinoma renal de células claras - Família EGFR - p53

Author's contribution:

H. L. D. P., E. F, F.C.A and A. C. U. conceived and planned the experiments, guided the students, contributed to the interpretation of the results and took the lead in writing the manuscript; I. C. A. S.; P. F. S. M; E. S. V. and T. L. S. carried out the experiment and contributed to the interpretation of the results. All authors provided critical feedback and helped shape the research, analysis and manuscript.




Publication History

Received: 20 February 2018

Accepted: 21 March 2018

Article published online:
11 June 2019

© 2022. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Bibliographical Record
Priscila Fernanda da Silva Martins, Izabella Cristina Alves Souza, Enio Ferreira, Emerson Soares Veloso, Tatiany L Silveira, Adam Underwood, Fabiano Conde Araujo, Helen Lima Del-Puerto. Expression of EGFR family and p53 in a patient with Synchronous Primary Endometrial Adenocarcinoma and Clear Cell Renal Carcinoma: a Case Report. Brazilian Journal of Oncology 2019; 15: e-20190011.
DOI: 10.5935/2526-8732.20190011
 

  • REFERENCES

  • Li F, Zhong WZ, Niu FY, Zhao N, Yang JJ, Yan HH. et al Multiple primary malignancies involving lung cancer. BMC Cancer 2015; 15: 696-703
    Search in Google Scholar
  • Ferri JVV, Gornatti VP, Martins TV.. Malignidades primárias sincrônicas de pulmão e reto: relato de caso e revisão da literatura. Revista de Medicina 2016; 95: 152-155
    Search in Google Scholar
  • Warren S, Gates O.. Multiple primary malignant tumors. Am J Cancer 1932; 16: 1358-1414
    Search in Google Scholar
  • Konecny GE, Venkatesan N, Yang G, Dering J, Ginther C, Finn R. et al Activity of lapatinib a novel HER2 and EGFR dual kinase inhibitor in human endometrial cancer cells. Br J Cancer 2008; 98 (06) 1076-1084
    Search in Google Scholar
  • Merseburger AS, Hennenlotter J, Simon P, Kruck S, Koch E, Horstmann M. et al Membranous expression and prognostic implications of epidermal growth factor receptor protein in human renal cell cancer. Anticancer Res 2005; 25: 19011908
    Search in Google Scholar
  • Cohen D, Lane B, Jin T, Magi-Galluzzi C, Finke J, Rini BI. et al The prognostic significance of epidermal growth factor receptor expression in clear cell renal cell carcinoma: A call for standardized methods for immunohistochemical evaluation. Clin Genitourin Cancer 2007; 5 (04) 264-270
    Search in Google Scholar
  • Reyes HD, Thiell KW, Carlson MJ, Meng X, Yang S, Stephan J-M. et al Comprehensive profiling of EGFR/HER receptors for personalized treatment of gynecologic cancers. Mol Diagn 3 Ther 2014; 18 (02) 137-151
    Search in Google Scholar
  • Srinivasan R, Benton E, McCormick F, Thomas H, Gullick WJ.. Expression of the c-erbB-3/HER-3 and c-erbB-4/HER-4 growth factor receptors and their ligands, neuregulin-1 alpha, neuregulin-1 beta, and betacellulin, in normal endometrium and endometrial cancer. Clin Cancer Res 1999; 5: 2877-2883
    Search in Google Scholar
  • Thomasson M, Hedman H, Junttila TT, Elenius K, Ljungberg B, Henriksson R.. ErbB4 is downregulated in renal cell carcinoma--a quantitative RTPCR and immunohistochemical analysis of the epidermal growth factor receptor family. Acta Oncol 2004; 43: 453-459
    Search in Google Scholar
  • Morrison C, Zanagnolo V, Ramirez N, Cohn DE, Kelbick N, Copeland L. et al HER-2 is an independent prognostic factor in endometrial cancer: association with outcome in a large cohort of surgically staged patients. J Clin Oncol 2006; 24: 2376-2385
    Search in Google Scholar
  • Stumm G, Eberwein S, Rostock-Wolf S, Stein H, Pomer S, Schlegel J. et al Concomitant overexpression of the EGFR and erbB-2 genes in renal cell carcinoma (RCC) is correlated with differentiation and metastasis. Int J Cancer 1996; 69: 17-22
    Search in Google Scholar
  • Wang H, Liu C, Han J, Zhen L, Zhang T, He X. et al HER2 expression in renal cell carcinoma is rare and negatively correlated with that in normal renal tissue. Oncol Lett 2012; 4: 194-198
    Search in Google Scholar
  • Dordevic G, Matušan Ilijaš K, Hadžisejdic I, Maricic A, Grahovac B, Jonjic N. EGFR. protein overexpression correlates with chromosome 7 polysomy and poor prognostic parameters in clear cell renal cell carcinoma. J Biomed Sci 2012; 19: 40
    Search in Google Scholar
  • Berchuck A, Soisson AP, Olt GJ, Soper JT, Clarke-Pearson DL, Bast RC. et al Epidermal growth factor receptor expression in normal and malignant endometrium. Am J Obstet Gynecol 1989; 161: 1247-1252
    Search in Google Scholar
  • Latif Z, Watters AD, Bartlett JMS, Underwood MA, Aitchison M.. Gene amplification and overexpression of HER2 in renal cell carcinoma. BJU Int 2002; 89: 5-9
    Search in Google Scholar
  • Niikura H, Sasano H, Kaga K, Sato S, Yajima A.. Expression of epidermal growth factor family proteins and epidermal growth factor receptor in human endometrium. Hum Pathol 1996; 27: 282-289
    Search in Google Scholar
  • Konecny GE, Santos L, Winterhoff B, Hatmal M, Keeney GL, Mariani A. et al HER2 gene amplification and EGFR expression in a large cohort of surgically staged patients with non-endometrioid (type II) endometrial cancer. Br J Cancer 2009; 100: 89-95
    Search in Google Scholar
  • Masjeed NMA, Khandeparkar SGS, Joshi AR, Kulkarni MM, Pandya N.. Immunohistochemical Study of ER, PR, Ki67 and p53 in Endometrial Hyperplasias and Endometrial Carcinomas. J Clin Diagn Res 2017; 11: 31-34
    Search in Google Scholar
  • Hodorova I, Solar P, Mihalik J, Vecanova J, Adamkov M, Rybarova S.. Investigation of tumour supressor protein p53 in renal cell carcinoma patients. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 2014; 158: 44-49
    Search in Google Scholar