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DOI: 10.5999/aps.2016.43.6.595
Microsurgical Training using Reusable Human Vessels from Discarded Tissues in Lymph Node Dissection
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The use of human vessels at the beginning of microsurgery training is highly recommended. But vessels with the appropriate length for training are not often obtained. Whether these vessels may be reused for training has not been reported. Accordingly, we harvested vessels from discarded tissues in lymph node dissection and demonstrated that vascular anastomosis training using the same human vessels several times is possible by placing the vessels in a freezer and defrosting them with hot water. Vascular walls can be stored for microsurgical training until about 4 years after harvest, as shown in the gross appearance and histologic findings of our preserved vessels. We recommend the technique presented here for the longterm reuse of human vessels for microsurgery training that closely resembles real procedures.
The authors would like to acknowledge Nobuo Hoshi, M.D., of the Department of Pathology, Tochigi Cancer Center for his pathological contribution to our study.
Publication History
Received: 07 July 2016
Accepted: 20 September 2016
Article published online:
20 April 2022
© 2016. The Korean Society of Plastic and Reconstructive Surgeons. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonCommercial License, permitting unrestricted noncommercial use, distribution, and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes. (https://creativecommons.org/licenses/by-nc/4.0/)
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References
- 1 Meier SA, Lang A, Beer GM. Polyurethane vessels for microvascular surgical training to reduce animal use. ALTEX 2004; 21: 135-138
- 2 Shurey S, Akelina Y, Legagneux J. et al. The rat model in microsurgery education: classical exercises and new horizons. Arch Plast Surg 2014; 41: 201-208
- 3 Krishnan KG, Dramm P, Schackert G. Simple and viable in vitro perfusion model for training microvascular anastomoses. Microsurgery 2004; 24: 335-338
- 4 Allouni A, Amer T, Ismail M. et al. The human umbilical cord: a model for microsurgical training. J Hand Microsurg 2014; 6: 110-112
- 5 Seyhan T, Ozerdem OR. Microsurgery training on discarded abdominoplasty material. Plast Reconstr Surg 2006; 117: 2536-2537