Download PDF
CC BY-NC 4.0 · Arch Plast Surg 2018; 45(02): 111-117
DOI: 10.5999/aps.2017.01487
Original Article

In vivo tracking of adipose tissue grafts with cadmium-telluride quantum dots

Claus J. Deglmann
Centre for Hand Surgery, Microsurgery and Plastic Surgery, Schoen-Klinik Muenchen Harlaching, Munich, Germany
Department of Hand-, Plastic- and Aesthetic Surgery, Klinikum Grosshadern, LMU Munich, Munich, Germany
,
Katarzyna Błażków-Schmalzbauer
Department of Hand-, Plastic- and Aesthetic Surgery, Klinikum Grosshadern, LMU Munich, Munich, Germany
,
Sarah Moorkamp
Department of Hand-, Plastic- and Aesthetic Surgery, Klinikum Grosshadern, LMU Munich, Munich, Germany
,
Jens Wallmichrath
Department of Hand-, Plastic- and Aesthetic Surgery, Klinikum Grosshadern, LMU Munich, Munich, Germany
,
Riccardo E. Giunta
Department of Hand-, Plastic- and Aesthetic Surgery, Klinikum Grosshadern, LMU Munich, Munich, Germany
,
Andrey L. Rogach
Centre of Functional Photonics (CFP), Department of Physics and Material Science, City University of Hong Kong, Hong Kong
Center for NanoScience (CeNS), LMU Munich, Munich, Germany
,
Ernst Wagner
Center for NanoScience (CeNS), LMU Munich, Munich, Germany
Center for System based Drug Research, Department of Pharmacy, Pharmaceutical Biotechnolgy, LMU Munich, Munich, Germany
,
Ruediger G. Baumeister
Department of Hand-, Plastic- and Aesthetic Surgery, Klinikum Grosshadern, LMU Munich, Munich, Germany
,
Manfred Ogris
Center for NanoScience (CeNS), LMU Munich, Munich, Germany
Center for System based Drug Research, Department of Pharmacy, Pharmaceutical Biotechnolgy, LMU Munich, Munich, Germany
Department of Clinical Pharmacy and Diagnostics, Centre of Pharmaceutical Sciences, University of Vienna, Vienna, Austria
› Author Affiliations
› Further Information
   Permissions and Reprints

Background Fat grafting, or lipofilling, represent frequent clinically used entities. The fate of these transplants is still not predictable, whereas only few animal models are available for further research. Quantum dots (QDs) are semiconductor nanocrystals which can be conveniently tracked in vivo due to photoluminescence.

Methods Fat grafts in cluster form were labeled with cadmium-telluride (CdTe)-QD 770 and transplanted subcutaneously in a murine in vivo model. Photoluminescence levels were serially followed in vivo.

Results Tracing of fat grafts was possible for 50 days with CdTe-QD 770. The remaining photoluminescence was 4.9%±2.5% for the QDs marked fat grafts after 30 days and 4.2%± 1.7% after 50 days. There was no significant correlation in the relative course of the tracking signal, when vital fat transplants were compared to non-vital graft controls.

Conclusions For the first-time fat grafts were tracked in vivo with CdTe-QDs. CdTe-QDs could offer a new option for in vivo tracking of fat grafts for at least 50 days, but do not document vitality of the grafts.

Keywords

Quantum dots - Adipocytes - Transplantation - Adipose tissue

Funding by DFG Excellence Cluster Nanosystems Initiative Munich (to E. Wagner) is gratefully acknowledged.


Parts of this work were presented at the DGPRAEC (German Society of Plastic, Reconstructive and Aesthetic Surgeons) meeting on September 18, 2010, in Dresden, Germany.




Publication History

Received: 16 September 2017

Accepted: 27 December 2017

Article published online:
22 May 2022

© 2018. The Korean Society of Plastic and Reconstructive Surgeons. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonCommercial License, permitting unrestricted noncommercial use, distribution, and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes. (https://creativecommons.org/licenses/by-nc/4.0/)

Thieme Medical Publishers, Inc.
333 Seventh Avenue, 18th Floor, New York, NY 10001, USA

 

  • REFERENCES

  • 1 Coleman SR. Structural fat grafting: more than a permanent filler. Plast Reconstr Surg 2006; 118(3 Suppl): 108S-120S
    CrossrefPubMedGoogle Scholar
  • 2 Duncker DJ, Uitterdijk A, Van der Giessen WJ. Fat is not all bad: how to make good use of adipose tissue. Eur Heart J 2007; 28: 2565-7
    CrossrefPubMedGoogle Scholar
  • 3 Sterodimas A, De Faria J, Correa WE, Pitanguy I. Tissue engineering in plastic surgery: an up-to-date review of the current literature. Ann Plast Surg 2009; 62: 97-103
    CrossrefPubMedGoogle Scholar
  • 4 Rogach AL, Ogris M. Near-infrared-emitting semiconductor quantum dots for tumor imaging and targeting. Curr Opin Mol Ther 2010; 12: 331-9
    PubMedGoogle Scholar
  • 5 Deglmann CJ, Blazkow-Schmalzbauer K, Moorkamp S. et al. Cadmium telluride quantum dots as a fluorescence marker for adipose tissue grafts. Ann Plast Surg 2017; 78: 217-22
    CrossrefPubMedGoogle Scholar
  • 6 Gaponik N, Talapin DV, Rogach AL. et al. Thiol-capping of CdTe nanocrystals: an alternative to organometallic synthetic routes. J Phys Chem B 2002; 106: 7177-85
    CrossrefGoogle Scholar
  • 7 Guerrerosantos J. Long-term outcome of autologous fat transplantation in aesthetic facial recontouring: sixteen years of experience with 1936 cases. Clin Plast Surg 2000; 27: 515-43
    CrossrefPubMedGoogle Scholar
  • 8 Bucky LP, Percec I. The science of autologous fat grafting: views on current and future approaches to neoadipogenesis. Aesthet Surg J 2008; 28: 313-21
    CrossrefPubMedGoogle Scholar
  • 9 Yoshimura K, Suga H, Eto H. Adipose-derived stem/progenitor cells: roles in adipose tissue remodeling and potential use for soft tissue augmentation. Regen Med 2009; 4: 265-73
    CrossrefPubMedGoogle Scholar
  • 10 Rieck B, Schlaak S. Measurement in vivo of the survival rate in autologous adipocyte transplantation. Plast Reconstr Surg 2003; 111: 2315-23
    CrossrefPubMedGoogle Scholar
  • 11 Rieck B, Schlaak S. In vivo tracking of rat preadipocytes after autologous transplantation. Ann Plast Surg 2003; 51: 294-300
    CrossrefPubMedGoogle Scholar
  • 12 Hemmrich K, Meersch M, von Heimburg D, Pallua N. Applicability of the dyes CFSE, CM-DiI and PKH26 for tracking of human preadipocytes to evaluate adipose tissue engineering. Cells Tissues Organs 2006; 184: 117-27
    CrossrefPubMedGoogle Scholar
  • 13 Zhou SB, Chiang CA, Xie Y. et al. In vivo bioimaging analysis of stromal vascular fraction-assisted fat grafting: the interaction and mutualism of cells and grafted fat. Transplantation 2014; 98: 1048-55
    CrossrefPubMedGoogle Scholar
  • 14 Bliley JM, Satish L, McLaughlin MM. et al. Imaging the stromal vascular fraction during soft-tissue reconstruction. Plast Reconstr Surg 2015; 136: 1205-15
    CrossrefPubMedGoogle Scholar
  • 15 Coleman SR. Facial recontouring with lipostructure. Clin Plast Surg 1997; 24: 347-67
    CrossrefPubMedGoogle Scholar
  • 16 Akerman ME, Chan WC, Laakkonen P, Bhatia SN, Ruoslahti E. Nanocrystal targeting in vivo. Proc Natl Acad Sci U S A 2002; 99: 12617-21
    CrossrefPubMedGoogle Scholar
  • 17 Zintchenko A, Susha AS, Concia M. et al. Drug nanocarriers labeled with near-infrared-emitting quantum dots (quantoplexes): imaging fast dynamics of distribution in living animals. Mol Ther 2009; 17: 1849-56
    CrossrefPubMedGoogle Scholar
  • 18 Yong KT, Roy I, Hu R. et al. Synthesis of ternary CuInS(2)/ZnS quantum dot bioconjugates and their applications for targeted cancer bioimaging. Integr Biol (Camb) 2010; 2: 121-9
    CrossrefPubMedGoogle Scholar
  • 19 Snyder EL, Bailey D, Shipitsin M, Polyak K, Loda M. Identification of CD44v6(+)/CD24- breast carcinoma cells in primary human tumors by quantum dot-conjugated antibodies. Lab Invest 2009; 89: 857-66
    Google Scholar
  • 20 Yukawa H, Mizufune S, Mamori C. et al. Quantum dots for labeling adipose tissue-derived stem cells. Cell Transplant 2009; 18: 591-9
    CrossrefPubMedGoogle Scholar
  • 21 Yukawa H, Kagami Y, Watanabe M. et al. Quantum dots labeling using octa-arginine peptides for imaging of adipose tissue-derived stem cells. Biomaterials 2010; 31: 4094-103
    CrossrefPubMedGoogle Scholar
  • 22 Smahel J. Experimental implantation of adipose tissue fragments. Br J Plast Surg 1989; 42: 207-11
    CrossrefPubMedGoogle Scholar
  • 23 Cho SJ, Maysinger D, Jain M, Roder B, Hackbarth S, Winnik FM. Long-term exposure to CdTe quantum dots causes functional impairments in live cells. Langmuir 2007; 23: 1974-80
    CrossrefPubMedGoogle Scholar
  • 24 Yong KT, Roy I, Ding H, Bergey EJ, Prasad PN. Biocompatible near-infrared quantum dots as ultrasensitive probes for long-term in vivo imaging applications. Small 2009; 5: 1997-2004
    CrossrefPubMedGoogle Scholar
  • 25 De Matteis V. Exposure to inorganic nanoparticles: routes of entry, immune response, biodistribution and in vitro/in vivo toxicity evaluation. Toxics 2017; 5: 29
    CrossrefPubMedGoogle Scholar