Key words
geraniol - essential oil - pharmacology - monoterpene
Abbreviations
4NQO:
4-nitroquinoline-1-oxide
ACR:
acrylamide
AD:
atherogenic diet
ALT:
alkaline phosphatase
AST:
aspartate aminotransferase
CUMS:
chronic unpredictable mild stress
DMBA:
7,12-dimethylbenz[a]anthracene
DSS:
dextran sulfate sodium
GE:
geraniol
GSH:
glutathione
GST:
glutathione S transferase
HMGCR:
3-hydroxy-3methylglutaryl coenzyme A reductase
iNOS:
inducible nitric oxide synthase
LCAT:
lecithin cholesterol acyl transferase
LDL:
low-density lipoprotein
LPO:
lipid peroxidation
MAPK:
mitogen-activated protein kinase
MetS:
metabolic syndrome
MPTP:
1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine
mRNA:
messenger ribonucleic acid
MS:
metabolic syndrome
NF-κB:
nuclear factor kappa-B
Nrf2:
nuclear factor E2-related factor 2
PCNA:
proliferating cell nuclear antigen
PD:
Parkinsonʼs disease
PH:
partial hepatectomy
PTEN:
phosphatase and tensin homolog deleted on chromosome ten
ROS:
reactive oxygen species
SCI:
spinal cord injury
STZ:
streptozotocin
TNBS:
trinitro-benzene-sulfonic acid
TPA:
12-O-tetradecanoylphorbol 13-acetate
VEGF:
vascular endothelial growth factor
VLDL:
very-low-density lipoprotein
Introduction
GE ([Fig. 1]), an acyclic isoprenoid monoterpene, can be extracted from the essential oils of several aromatic plants, such as Cinnamomum tenuipilum Kosterm (Lauraceae), Valeriana officinalis L. (Caprifoliaceae), Phyla scaberrima (Juss. ex Pers.) Moldenke (Verbenaceae), and Perilla frutescens var. hirtella (Nakai) Makino (Lamiaceae) [1]. GE has been widely used in the flavor and fragrance industries in the past decades. However, the limited production of GE via plant extraction restrains market growth. Thus, several studies are aimed at identifying an effective method to biosynthesize GE.
Fig. 1 The chemical structure of geraniol using ChemBioDraw.
GE production maximization was achieved in Escherichia coli engineered to express GE synthase and with the YjgB mutation, resulting in an increase in geranyl diphosphate and prevention of GE dehydrogenation, yielding 183 mg/L in 2014 [2]. GE production was further improved during both autotrophic and heterotrophic growth in engineered Methanococcus maripaludis with cloned GE synthase from sweet basil Ocimum basilicum and the absence of puromycin, reaching 4.6 mg/g of dry weight [3]. Finally, the highest production of GE was obtained in Saccharomyces cerevisiae with GE synthase and arnesyl diphosphate synthase expression, reaching a yield of 1.68 g/L in 2017 [4].
Recently, GE was shown to possess various pharmacological properties, including antioxidant [5], anti-inflammatory [6], antimicrobial [7], and antitumor activities [8], through multiple signaling pathway regulation in diverse biological processes, suggesting that GE is a promising drug candidate. To provide novel insights and support the clinical use of GE, we summarize the pharmacological activities of GE based on PubMed, ScienceDirect, Springer, and Wiley database searches. The scientific literature published in the years 2013 to 2018 was searched. However, several papers published before 2013 were also included for insight in the introduction and explanation. We used GE either alone or combined with antioxidant, anti-inflammatory, antimicrobial, antitumor, hepatoprotective, cardioprotective, and neuroprotective as the key words for the literature searches. Initially, 1569 research papers were selected from with the above search strategy using a ScienceDirect database search. Only 944 English written original articles about pharmacological effects and production of GE were included, refined by the Wiley database. Articles also in the PubMed database or Springer database were collected in advance. Duplicate articles were excluded and articles with similar results were also excluded. Only 72 articles were finally included after reading the titles, abstracts, and whole papers ([Fig. 2]).
Fig. 2 Criteria in this review for article searches and selection.
Cytotoxic and Antitumor Activities
Cytotoxic and Antitumor Activities
GE exerted cytotoxic and antitumor activities on various types of cancers ([Table 1]). Colon cancer ranks as the fourth leading cause of cancer mortality worldwide [9]. A recent study demonstrated that GE could significantly inhibit cell growth in the colon cancer cell line colo-205 with an IC50 value of 20 µM. DAPI staining indicated that GE induces apoptosis, and a Western blot assay confirmed that GE upregulates Bax and downregulates Bcl-2. Bax is a pro-apoptosis regulator and Bcl-2 is an anti-apoptosis regulator. Additionally, GE induces DNA damage and cell cycle arrest in colo-205 cells [10]. Endometrial carcinoma is a common cancer among women worldwide [11]. It is worthwhile to search for novel drug candidates to treat endometrial carcinoma. Recent research showed that GE treatment and pretreatment could suppress endometrial carcinoma by inhibiting oncogenes and activating tumor suppressor genes in a female Wistar rat model induced by N-methyl-N′-nitro-N-nitrosoguanidine at 150 mg/kg. The suppression effect of GE was more effective in the pretreatment group. Real-time PCR results show that GE inhibits the transcription of K-ras, MAPK, PI3K, and β-catenin, and Western blot results show that GE increases phosphatase and PTEN, progesterone receptors, and E-cadherin protein expression. MAPKs includes JNK, p38, and ERKs [12]. K-ras, MAPK, PI3K, and β-catenin are oncogenes, and PTEN, progesterone receptors, and E-cadherin are tumor suppressive genes [13]. The endothelioma cell line eEND2 isolated from hemangiomas easily develops angiogenesis [14]. A recent study exerted that GE showed an anti-angiogenesis effect on the endothelioma cell line eEND2. GE inhibits the migratory activity of endothelial-like eEND2 cells, along with inhibition of PCNA expression. Furthermore, GE can block the transduction of the VEGF/VEGFR-2 signal. VEGF promotes angiogenesis in cancer cells. The effect on angiogenesis was verified in vivo: GE administration to BALB/c mice reduces the number of Ki67-positive cells and CD31-positive microvessels through attenuating VEGFR-2 expression [15].
Table 1 The cytotoxic and antitumor activities of GE.
|
Cancer type
|
Suppressive effect
|
Cellular processes
|
Function study
|
Reference
|
|
Colon cancer
|
Anti-proliferation
|
Apoptosis, DNA damage, and cell cycle arrest
|
In vitro
|
[10]
|
|
Endometrial carcinoma
|
Anti-migration
|
Antiangiogenesis
|
In vitro and in vivo
|
[15]
|
|
Liver cancer
|
Anti-proliferation
|
Apoptosis
|
In vitro and in vivo
|
[17], [18], [19]
|
|
Lung cancer
|
Anti-proliferation
|
Apoptosis
|
In vitro
|
[21]
|
|
Pancreatic cancer
|
Anti-proliferation
|
Apoptosis
|
In vitro
|
[26]
|
|
Prostate cancer
|
Anti-proliferation
|
Cell cycle arrest
|
In vitro
|
[28]
|
|
Skin cancer
|
Anti-proliferation
|
Apoptosis
|
In vivo
|
[30]
|
Primary liver cancer is the third leading cause of cancer death worldwide [16]. Pure compounds isolated from natural products exerted a cytotoxic effect on liver cancer cells. In Hep-G2 hepatic cells, GE exerts an inhibitory effect by promoting apoptosis. Also, GE inhibits HMGCR protein and mRNA expression, leading to a cholesterogenesis decrease [17]. Regarding an effect of GE on hepatic cell proliferation, GE significantly inhibits Hep-G2 proliferation at a concentration as low as 5 µg/mL [18]. Also, GE shows an inhibitory effect on diethylnitrosamine-associated hepatocarcinogenesis after a 12-week treatment, as shown by the reduction of AST and ALT levels in serum. Additionally, GE demonstrates a suppressive effect on both PCNA and GST-positive area ratios [19].
Lung cancer ranks first as the cause of mortality among men and third among women worldwide [20]. Oral administration to nude mice of 25, 50, and 75 mmol/kg GE inhibits lung cancer cell growth in vivo via an increase of cell apoptosis. This later study shows that GE downregulates HMGCR levels and decreases membrane-bound Ras protein amounts, leading to a decrease in cholesterogenesis. Via the suppressive effect of GE on the mevalonate pathway, cell proliferation is inhibited, and apoptosis is promoted in A549 tumors compared with normal cells where GE did not show any inhibitory effect on cell survival [21].
Oral cancers account for 2 – 3% of cancers worldwide, but their survival rate is poor [22]. Thus, it is urgent to search for a novel drug candidate to treat oral cancer. In the 4NQO-induced cancerous mouse model, GE decreased the incidence of oral cancer as a chemopreventive agent. Via the NF-κB pathway, GE reduces the density of immature and mature mast cells and downregulates the expression of downstream inflammatory mediators (e.g., TNF-α, IL-1β, COX-2, and iNOS) in this model [23]. Another study showed the suppressive effect of GE being effective at a concentration of 200 mg/kg administered orally in the 4NQO-induced mouse model, which results in the inhibition of the Nrf2 pathway and drug metabolizing enzymes [24].
Pancreatic cancer ranks as the fourth leading cause of cancer mortality in Western countries [25]. Accumulating evidence suggests that natural products exerts a cytotoxic effect on pancreatic cancer cells. In BXPC-3 human pancreatic carcinoma cells, GE significantly suppresses proliferation and induces apoptosis in a dose- and time-dependent manner. Pretreatment with GE for 24 h before gemcitabine treatment shows a maximal inhibition in BXPC-3 cells. This study also reports that GE suppresses proliferation of prostate cancer cells by inducing ROS production and downregulating phosphorylation of tyrosine kinase [26].
Prostate cancer remains the first cause of mortality in the United States [27]. In prostate tumor cells, GE administration inhibits growth and survival by downregulating the expression of transcription factor E2F8. Via the inhibition of this pivotal cell cycle control factor, the gene expression profile of genes involved in the cell cycle was altered, and the E2F8 inhibition had a suppressive effect on cell growth via inducing G2/M arrest [28].
Skin cancer incidences have been increasing in the last decades [29]. Hence, it is worthy to identify a novel drug candidate to treat skin cancer. Recent reports show that GE significantly inhibits DMBA/TPA-mediated skin tumorigenesis in Swiss albino mice. GE suppresses the Ras/Raf/ERK1/2 signaling pathway and induces apoptosis [30].
Anti-inflammatory and Antioxidant Activities
Anti-inflammatory and Antioxidant Activities
Inhibition of the inflammatory response and oxidative stress prevents organs from damage, especially in the brain. The LPO and inflammatory reaction caused by TPA can be significantly decreased by GE. Further investigation demonstrated that GE promotes the metabolism of inflammatory cells, increases GSH content, and stimulates antioxidant enzyme activities. Furthermore, GE exerts an inhibitory effect on the alteration of p38MAPK activity and alleviates the expression change of NF-κB and COX-2 caused by TPA [31]. Another study also exerted that GE could inhibit inflammation through the inhibition of COX-2 [32]. GE has an inhibitory effect on the pamidronate-induced inflammatory response by stimulating IL-10 production. Also, GE showed no toxin effect on monocytes and did not impact the production of TNF-α
[33].
A study tested biochemical parameters and hepatic oxidative stress after GE treatment on Wistar rats and concluded that GE could work as an antioxidant during aromatherapy. GE administration was negatively correlated to the total cholesterol level but there was no difference in glycemia, triacylglycerol protein, and urea levels. The GE-treated rats showed an increase of GSH peroxidase and superoxide dismutase activity, leading to more peroxide accumulation in the liver. GE is able to upregulate the activity of ALT by promoting oxidative stress but has no remarkable effect on AST activity [34]. GE was also shown to protect hamsters from AD-induced abnormalities. AD-induced hamsters treated with 100 mg/kg GE showed that the underlying mechanism consists of alleviating the change in the lipid profile and endothelial function marker, promoting antioxidant enzyme activities and abating alterations in the LPO markers and Nrf2 expression. Histologically, GE negatively affected oxidative stress by increasing the activity of Nrf2 [35]. In the meantime, GE could exert anti-inflammatory and antioxidant effects.
Ulcerative colitis severely impairs the quality of life of patients, and no definitive standard of care has been proven effective, to date, for this indication. GE administration decreases the intestinal damage caused by DSS, as reflected in disease activity index scores such as colon length and stool consistency. Pretreatment with GE showed an inhibitory effect on the amount of proinflammatory cytokines and the activity of myeloperoxidase in colon tissue. GE downregulated the expression of downstream target proinflammatory enzymes but upregulated antioxidant molecules: GSH and superoxide dismutase activity were suppressed and LPO and nitrites increases were prevented [36].
GE oral administration was deemed to protect liver tissue from cancer by abating oxidative stress, inflammation, hyperproliferation, and apoptotic tissue damage induced by 2-acetylaminofluorene. Additionally, GE administration restored the damaged structure of the liver tissue. Regarding molecular mechanisms, GE decreased tissue LPO but increased enzymatic activities of catalase, GSH peroxidase, GSH reductase, and superoxide dismutase. GE inhibited the expression of caspase-3, caspase-9, COX-2, NF-κB, PCNA, iNOS, and VEGF, and disintegration of DNA was remarkably reduced [37]. GE could also decrease MetS-induced inflammation and free radical injury by suppressing visceral adiposity and decreasing fasting blood glucose and the glycemic excursion [38].
Another study showed that GE could prevent the ecological disturbances associated with colitis and reduce the inflammatory systemic profile in a colitis mouse model induced by DSS, suggesting that the clinical symptoms of colitis could be improved by GE. Further, the study showed that GE attenuated COX-2 expression in the gut wall [39]. The SCI rat model was used to study the mechanism of anti-inflammatory and antioxidant effects of GE and showed that GE negatively affected spinal cord water content and increased Beattie and Bresnahan scores when rats were treated for 4 weeks with 250 mg/kg/d GE. Further, the inhibitory effect of GE on inflammation and oxidative stress was mediated by attenuating the NF-κB and p38 MAPK pathways [40].
Antimicrobial Activity
Candida albicans is a human fungal pathogen leading to immunocompromized patients and a high rate of mortality [41]. A recent study showed that treatment with GE weakened the activities of albicans and non-albicans species of Candida. Oral administration of GE showed an inhibitory effect on the biofilm formation of pathogenic fungi and hyphal morphogenesis. GE destroyed the cell wall function by downregulating the activity of plasma membrane ATPase and reducing ergosterol levels. GE suppressed fungal expansion by promoting the sensitivity of a calcineurin signaling mutant while enhancing drug resistance to the calcineurin overexpressing strain. Additionally, GE destroyed the mitochondrial function, broke the iron homeostasis, and mitigated genetic toxicity [42]. Another study showed that the minimum inhibitory concentration of GE is 16 µg/mL against C. albicans and the antimycotic effect of GE is concentration dependent. The morphological interference test demonstrates that GE shows a suppressive effect on pseudohyphae and chlamydoconidia formation [43].
Trichophyton rubrum is one of the most common fungi found in chronic dermatophytosis cases [44]. GE could treat T. rubrum infections of the skin via its antifungal activity. A high concentration of GE could suppress mycelial and fungal growth, and also inhibit conidia germination. GE treated the T. rubrum infection mostly through leakage of the intracellular material and inhibition of ergosterol biosynthesis to destroy the cell wall and cell membrane [45].
Hepatoprotective Effects
GE could alleviate non-alcoholic steatohepatitis in a methionine-choline-deficient diet-induced rat model through 200 mg/kg GE oral supplement. In this model, GE administration showed an inhibitory effect on histological scores, fibrosis, and apoptosis in the liver. On the molecular level, GE inhibits the activities of alanine aminotransferase and aspartate aminotransferase in serum. GE protects the hepatic mitochondrial function through suppression of hepatic mitochondrial ROS, mitochondrial electron transport chain enzyme activity, and mitochondrial DNA content. Further, GE administration attenuates malondialdehyde and 3-nitrotyrosine formation, and inhibits the expression of iNOS and cytochrome P450 2E1 in the liver. Moreover, GE shows an inhibitory effect on inflammation in the liver with the inhibition of TNF-α and IL-6 expression and myeloperoxidase activity [46]. According to hematoxylin and eosin staining and PCR results, GE exhibits a hepatoprotective effect in rats after a 70% PH, as intraperitoneal administration of GE promoted liver tissue regeneration via improving the expression levels of TNF-α and IL-6. Also, GE remarkably suppressed the ALT level in serum, which regulates the function recovery of liver cells [47]. GE is a natural compound remarkably stimulating liver regeneration as shown in the rat model with a PH that was injected with GE 100 mg/kg. Regarding the mechanism, GE increases the expression of NF-κB, TNF-α, and IL-6 mRNA and protein levels, and inhibits HSP27 and HSP60 protein levels [48]. GE could partly restore the oxidative hepatic damage through the administration of a 50 mg/kg GE intraperitoneal injection in a H2O2-induced oxidative stress rat model. The mechanism entails the regulation by GE of malondialdehyde and GSH variations caused by H2O2 and a suppressive effect on hepatic catalase activities [49].
Cardioprotective Effects
GE could alleviate hyperlipidemia and reduce the incidence of coronary heart disease in an AD feeding mouse model. GE reduces blood fat via the downregulation of HMGCR and the inhibition of lipogenesis. GE suppresses LCAT, decreasing cholesterol esters levels. At the same time, treatment with GE increases the activity of C-reactive protein [50]. GE protects the cardiovascular system from body injury caused by high blood lipids. In particular, GE treatment reduces total cholesterol and total triglyceride production in the plasma, and inhibits the biosynthesis of hepatic fatty acids, total lipids, and non-saponifiable lipids. GE increases the uptake of serum LDL by increasing LDL mRNA and VLDL receptor mRNA. Finally, GE suppresses triglyceride synthesis with a decrease of fatty acid synthesis [51]. Over the years, attention has shifted toward GE as a candidate for the treatment of endothelial cell function disorder resulting from a high-fat diet. In addition, GE downregulates serum thiobarbituric acid reactive substances and aortic ROS production. A GE supplement could protect high-fat diet-induced vascular endothelial dysfunction by inhibiting NOX-2 expression in the aorta [52]. GE inhibits ROS levels and reduces the injury during myocardial ischemia-reperfusion, and promotes the function and viability of myocardial cells. GE administration in neonatal rat ventricular cardiomyocytes decreased the endogenous production of ROS and exerted a positive modulation of pAMPK levels while attenuating pERK1/2 levels (pAMPK and pERK1/2 are key factors regulating cell survival). Hence, GE showed a protective effect on myocardial cells [53]. GE exerted a cardioprotective effect against cardiac dysfunction induced by diabetes by inhibiting oxidative stress and increasing the marker of oxidative stress 8-isoprostane [54].
Neuroprotective Effects
In the Drosophila model of ACR-induced nerve injury, GE shows a neuroprotective effect, as mortality caused by ACR is decreased significantly. GE supplementation can attenuate ACR-induced oxidative stress, mitochondrial dysfunction, and neurotoxicity. The mechanism involves the recovery of GSH and total thiol levels with an increase of detoxifying enzymes activities. In addition, coadministration of GE with curcumin showed a negative effect on cholinergic function with a decrease of acetylcholinesterase activity [55]. PD refers to a long-term degenerative disorder of the central nervous system affecting the motor system. GE pretreatment had a protective effect on the nervous system damage caused by PD in a MPTP-induced PD mouse model. GE oral administration ameliorated the neuromuscular disorder via the increase of tyrosine hydroxylase immunoreactive expression and the decrease of α-synuclein expression [56]. GE could reduce the motor behavior and neurotrophic factors inadequacy in an MPTP-induced PD mouse model [57]. Another study demonstrated that GE administration in the MPTP-induced PD mouse model regulated the Bcl-2/Bax ratio and inhibited the expression of cytochrome c and caspase-9, resulting in alleviating the neurodegeneration and disordered movements. Cytochrome c binds to cardiolipin to release it out of the mitochondria and initiate apoptosis. Caspase-9 is an enzyme initiating the apoptotic pathway [41]. GE administration exerts an inhibitory effect on the PD clinical symptoms of transgenic PD model Drosophila. GE regulates oxidative stress through a decrease of GSH content and an increase of protein carbonyl content, LPO, and GST activity. GE significantly inhibits α-synuclein expression, leading to the attenuation of the PD clinical symptoms in flies [58]. In rats with SCI, GE could attenuate the neuropathic pain and functional impairment through GE administration. Moreover, GE had a positive effect on NeuN-positive cells, but inhibited the expression of glial fibrillary acidic protein in the damaged area and decreased the activity of caspase-3. Further, the study demonstrated that GE treatment in SCI rats decreased malondialdehyde and 3-nitrotyrosine levels, promoted the protein expression of nuclear factor erythroid 2-related factor 2 and heme oxygenase 1, and decreased the expression of iNOS. In summary, GE exerts a positive effect on SCI recovery and alleviates neuropathic pain. Hence, GE represents a candidate drug for SCI treatment [59].
Antidiabetic Effects
Diabetes is a metabolic disorder presenting with high blood sugar levels and potentially leading to various complications. In an STZ-induced diabetic rat model, GE could be used to treat diabetes, as GE administration for 45 days decreased plasma glucose and hemoglobin HbA1C and could restore the insulin response. The activities of many enzymes involved in glucose production and utilization were restored, leading to an amelioration in carbohydrate metabolism and the restoration of glucose homeostasis [60]. Another study in an STZ-induced diabetic rat model showed that GE treatment has a protective effect on diabetic neuropathy and improved the sensory and motor functions by decreasing enzyme activities (i.e., complexes I – III, succinate dehydrogenase, and citrate synthase) in the sciatic nerve cytosol and selected regions of the brain. GE could alleviate cytosolic calcium levels and acetylcholinesterase activity. Meanwhile, GE could inhibit the protein of carbonyls and nitrites [61]. GE attenuates another diabetic induced complication, i.e., diabetes-induced cardiac functional disorder, by inhibiting oxidative stress and decreasing hyperglycemia. The treatment with GE could increase the cardiac systolic function and recover the diastolic function injured by hyperglycemia. In addition, GE inhibited dramatic oxidative stress by preventing 8-isoprostane increases [62]. GE could attenuate the vascular responsiveness damage of mice with diabetes or MS. Namely, GE administration improved the excessive vasoconstriction induced by diabetes or MS exposed to methylglyoxal in a dose-dependent manner. The study further demonstrated that preincubation with GE ameliorates vasoconstriction. In addition, GE had a suppressive effect on vasoconstriction induced by voltage-dependent and receptor-mediated calcium channels. GE reduces the reaction of the damaged vessels by blocking these types of calcium channels [63].
Other Effects
GE shows an antiarrhythmic effect on ouabain-induced arrhythmias in mammalian models. GE alleviates ouabain-induced arrhythmias through the downregulation of the contractile force of the heart. GE administration blocks the transient outward potassium (K+) current (59.7%), the non-inactivation K+ current (39.2%), and the inward rectifier K+ current (33.7%), leading to an action potential duration, which increased by 50%. GE also decreased left ventricular pressure (83%) and the heart rate (16.5%). GE also prolonged the onset of ouabain-induced arrhythmias by 128% and decreased the resting tension by 30% [64].
GE shows excellent anti-ulcerogenic activity on gastric and duodenal mucosa. After GE oral administration to rats, a dose of 7.50 mg/kg GE induces an increase of GSH content and a decrease of myeloperoxidase levels. Moreover, GE reduces the ischemia/reperfusion-induced duodenal ulcerative injury by 71% and cysteamine-induced duodenal ulcers by 68%. GE protects the gastric and duodenal mucosa by enhancing the activities of protective factors, including endogenous prostaglandins and the nitric oxide pathway. The protection mechanism also involves the increase of mucus secretion with antioxidant properties. GE increases the release of calcitonin gene-related peptide [65]. GE has a protective effect on TNBS-induced colitis injury. Specifically, TNBS-induced colitis rats treated with GE for 11 days showed an improvement of clinical symptoms (e.g., weight loss, colon edema, ulcerative area, and colon/spleen mass indices). GE and/or sulfasalazine could prevent the levels of nitric oxide and lipid peroxide from rising, and restored antioxidant activity in the colitis rat model. On the molecular level, GE inhibits apoptosis and inflammatory cell infiltration by reducing the levels of caspase-3, intercellular adhesion molecule-1, and myeloperoxidase. Intercellular adhesion molecule-1 is a transmembrane protein, and could stabilize cell-cell interactions and facilitate leukocyte endothelial transmigration. Myeloperoxidase oxidation reduces inhibition of apoptosis and inflammation. Conversely, the anti-inflammatory effect of GE was related to the downregulation of the colon contents of prostaglandin E2 and IL-1β
[66].
GE oral administration for 3 weeks showed an antidepressant-like effect on CUMS in rat models and greatly shortened the immobile time in tail suspension tests. More specially, GE decreased CUMS-induced proinflammatory IL-1β levels, resulting in the amelioration of the depressive behavior. GE treatment showed a suppressive effect on NF-κB pathway activation. Furthermore, GE adjusted nucleotide binding and the expression of oligomerization domain-like receptor family pyrin domain-containing 3 inflammasomes [67]. Treatment with GE (100 mg/kg b.w) attenuates atherosclerosis-induced tissue fibrosis by hindering tissue remodeling via the suppression of transcription factor NF-κB. The weak expression of NF-κB alleviates oxidative stress and inflammatory response, showing a protective effect on tissue fibers [68].
GE could treat Trichomonas vaginalis by killing the parasite. GE destroyed the nuclear membrane and nuclei, leading to chromatin accumulation and a large number of vacuoles appearing in the cytoplasm. Also, ribosomes were reduced, organelles were disintegrated, and the cell membrane was even damaged. Interestingly, GE did not show an inhibitory effect in a time-dependent manner [69].
GE administration at a dose of 100 or 200 mg/kg alleviated allergic asthma in an ovalbumin-sensitized rat model. The study showed that GE had an inhibitory effect on eotaxin levels in bronchoalveolar lavage fluid and infiltration of eosinophils. Namely, GE intervention decreased TH2 cytokines but stimulated TH1 cytokine interferon in bronchoalveolar lavage fluid. Moreover, GE promoted the expression of Nrf2 protein and intensified Nrf2-directed antioxidant pathways (e.g., glutamate-cysteine ligase, superoxide dismutase, and GST). GE also increased the production of GSH [70]. In experimental mice models, GE showed an antinociceptive activity following pain caused by inflammation. The antinociceptive activity was due to a decrease in peripheral nerve excitability. Particularly, the behavioral test showed that GE treatment reduced the number of writhes induced by acetic acid. In addition, GE administration reduced mice paw licking time through the regulation of glutamatergic neurotransmission. GE treatment could relieve pain by suppressing neurotransmitters and mediators of inflammation production [71]. Also, GE showed gastroprotective and gastric healing effects on acute ethanol-induced ulcers and chronic acetic acid-induced ulcers [72].
Conclusion
Recently, pure compounds from natural products are gaining acceptance as potentially promising complementary and alternative medicines for the treatment of various diseases. Generally, single compounds could target DNA, mRNA, protein, and even microRNA. Accumulating evidence in last the decades has indicated that GE is a pure botanical compound without adverse effects, exerting diverse activities by mainly regulating protein expression, suggesting that GE could become a novel drug candidate to treat various diseases. The cytotoxic effect of GE on cancer cells indicates that GE could treat cancer and reduce the mortality of cancer patients. The anti-inflammatory and oxidative effects of GE indicates that GE could protect organ damage and treat ulcerative colitis. The antifungal activity of GE indicates that GE could protect patients from fungal infection. The antidiabetic effect of GE indicates that GE could treat diabetes. The antinociceptive activity of GE indicates that GE could be used as an analgesic in clinical trials. However, anticancer effects of GE should be clarified in vivo in more animal models, and later in human patients, to confirm the inhibitory effect of GE on malignancy. Also, it is worthwhile to explore the molecular basis underlying pharmacological actions (e.g., antimicrobial and antiarrhythmic activities). The lack of information about definitive targets of GE would be a reason against the application of GE in clinical trials.
