Introduction
The genus Ballota , belonging to Lamiaceae family (Stachyoideae/Lamioideae subfamily) [1 ], [2 ], is, apart from the South African endemic species Ballota africana (L.) Benth., naturally distributed in the Mediterranean, the Middle East and in North Africa. Some species (e.g., Ballota nigra L. s. l.) are also present over large areas of western, central, and northern Europe, and 4 species, whose status will be discussed later, in Somalia.
Ballota species are perennial herbs or small shrubs with branched and/or simple hairs, toothed and petiolate leaves, the inflorescence thyrsoid or racemoid sometimes has long and spinose bracteoles (sect. Acanthoprasium), and the calyx is mostly campanulate, purple to white.
A former classification of the genus identified the occurrence of 31 species (1 = Ballota integrifolia Benth. – 2 = Ballota wettsteinii Rech. pat. – 3 = Ballota frutescens (L.) Woods – 4 = Ballota fruticosa Baker – 5 = Ballota somala Patzak – 6. Ballota andreuzziana Pamp. – 7 = Ballota acetabulosa (L.) Benth. – 8 = Ballota undulata (Sieb, ex Fres.) Benth. – 9 = Ballota pseudodictamnus (L.) Benth. – 10 = Ballota damascena Boiss. – 11 = Ballota hildebrandtii Vatke et Kurtz – 12 = Ballota hirsuta Benth. – 13 = Ballota bullata Pomel – 14 = B. africana (L.) Benth. – 15 = Ballota aucheri Boiss. – 16 = Ballota macrodonta Boiss. et Bal. – 17 = Ballota larendana Boiss. et Heldr. – 18 = Ballota rotundifolia C. Koch – 19 = Ballota rupestris (Biv.) Vis. – 20 = Ballota macedonica Vand. – 21 = Ballota kaiseri V. Täckh. – 22 = Ballota antilibanotica Post – 23 = Ballota cristata Davis – 24 = Ballota semanica Rech. f. – 25 = Ballota labillardieri Briq. – 26 = Ballota saxatilis Sieb. ex J. et C. Presl – 27 = Ballota stachydiformis Höchst. – 28 = Ballota philistea Bomm. – 29 = Ballota platyloma Rech. f. – 30 = B. nigra L. – 31 = Ballota royleoides Benth.) which were divided into 10 sections [3 ], [4 ], [5 ].
In subsequent years, several modifications and additions were made to the former classification.
The 4 Somalian species, B. fruticosa, B. somala, B. hildebrandtii , and B. stachydiformis were moved to other genera and now their accepted names are Otostegia modesta S. Moore, Isoleucas somala (Patzak) Scheen (syn. Otostegia somala (Patzak) Sebald), Otostegia hildebrandtii (Vatke & Kurtz) Sebald, and Leucas stachydiformis (Benth.) Hochst. ex Briq., respectively [6 ]; B. integrifolia and B. wettsteinii are both considered synonyms of Acanthoprasium integrifolium (Benth.) Ryding (accepted name) [7 ]; B. frutescens, B. labillardieri, B. semanica , and B. rupestris are synonyms of Acanthoprasium frutescens (L.) Spenn. [7 ], B. saxatilis, B. saxatilis subsp. brachyodonta (Boiss.) P. H. Davis & Doroszenko, and Ballota hispanica (L.) Benth., respectively [6 ].
The Plant List [6 ], which has been used to validate the scientific names of the species, includes more than 160 scientific plant names of species rank for the genus Ballota . Of these, only 30 are accepted species names.
The genus Otostegia (Lamiaceae family, Stachyoideae/Lamioideae subfamily), closely related to genus Ballota morphologically, with about 15 species, occurs in rather dry, often montane areas and semideserts [8 ]. There are 2 clearly disjoint centers of diversity for Otostegia : Central Asia to Afghanistan and northeastern Africa [9 ], although the genus is distributed from Cameroon to Saudi Arabia, Yemen, Egypt, Iran, and Central Asia to India [8 ].
In 2007, Scheen & Albert [10 ] proposed to restrict the Otostegia genus, including only the following 11 species in it: Otostegia ellenbeckii Gürke, Otostegia ericoidea Ryding, Otostegia erlangeri Gürke, Otostegia fedtschenkoana Kudr., Otostegia fruticosa (Forssk.) Schweinf. ex Penzig, O. hildebrandtii, Otostegia migirtiana Sebald, O. modesta, Otostegia olgae (Regel) Korsh., Otostegia sogdiana Kudr., and Otostegia tomentosa A. Rich. The former members of Otostegia, O. somala and Otostegia aucheri, were transferred to Isoleucas and Moluccella , respectively. A new genus was erected for the 4 yellow-flowered species of Otostegia (Otostegia integrifolia Benth., Otostegia limbata [Benth.] Boiss, Otostegia michauxii Briq., Otostegia persica (Burm. f.) Boiss), for which the name Rydingia A.-C. Scheen & V. A. Albert was proposed. Consequently, the 4 names actually accepted for these species are Rydingia integrifolia (Benth.) Scheen & V. A. Albert, Rydingia limbata (Benth.) Scheen & V. A. Albert, Rydingia michauxii (Briq.) Scheen & V. A. Albert, Rydingia persica (Burm. f.) Scheen & V. A. Albert [6 ].
The Plant List [6 ] is generally in agreement with this classification of genus Otostegia . It reports 46 name records of species rank for the genus Otostegia , of which only 10 species and 3 subspecies are accepted. In addition to the above-mentioned species [10 ], the Plant List added Otostegia nikitinae Scharasch. and Otostegia schennikovii Scharasch., while moving Otostegia bucharica B. Fedtsch., O. fedtschenkoana, O. olgae , and O. sogdiana to genus Moluccella
[7 ].
In this review, a complete survey of the traditional uses, chemical constituents (both volatile and nonvolatile), and biological properties of species from the genera Ballota and Otostegia is provided.
The available information on these genera was collected from scientific databases and cover from 1911 up to 2018. The following electronic databases were used: PubMed, SciFinder, Science Direct, Scopus, Web of Science, and Google Scholar.
The search terms used for this review included Ballota, Otostegia , all the botanical names of the species, both accepted names or synonyms, phytochemical composition, EOs, traditional uses, activity, pharmacology, and toxicity. No limitations were set for languages. [Table 1 ] reports the taxa of Ballota and Otostegia investigated so far, their synonyms, and the accepted botanical names.
Table 1 Ballota s. l. and Otostegia s. l. taxa studied so far and their synonymous (accepted botanical name in bold).
Taxa
Synonyms
Ballota acetabulosa (L.) Benth.
Ballota africana (L.) Benth.
Ballota andreuzziana Pamp.
Ballota antalyensis Tezcan & H. Duman
Ballota arabica Hochst. & Steud.
Leucas urticifolia (Vahl) Sm.
Ballota aucheri Boiss.
Otostegia aucheri Boiss.
Ballota cinerea D. Don
Roylea cinerea (D. Don) Baill. ; Roylea calycina (Roxb.) Briq.; R. elegans Wall. ex Benth.
Ballota cristata P. H.Davis
Ballota deserti (Noë) Jury, Rejdali & A. J. K.Griffiths
Marrubium deserti (Noë) Coss.
Ballota glandulosissima Hub.-Mor. & Patzak
Ballota hirsuta Benth.
Ballota hispanica (L.) Benth.
Ballota rupestris (Biv.) Vis.
Ballota inaequidens Hub.-Mor. & Patzak
Ballota lanata L.
Panzerina lanata (L.) Soják ; Panzeria alaschanica Kuprian.; P. lanata (L.) Bunge
Ballota larendana Boiss. & Heldr.
Ballota latibracteolata P. H. Davis & Doroszenko
Ballota macrodonta Boiss. & Balansa
Ballota nigra L.
B. nigra L. subsp. anatolica P. H.Davis
Ballota nigra subsp. foetida (Vis.) Hayek
Ballota nigra f. uncinata Beg,
Ballota nigra subsp. ruderalis (Sw.) Briq.
Ballota philistaea Bornm.
Ballota pilosa Lour.
Leucas chinensis (Retz.) Sm. ; L. mollissima subsp. chinensis (Benth.) Murata
Ballota pseudodictamnus (L.) Benth.
Ballota pseudodictamnus subsp. lycia Hub.-Mor
Ballota rotundifolia K. Koch
Ballota rupestris (Biv.) Vis.
Ballota hispanica (L.) Benth.
Ballota saxatilis Sieber ex C.Presl
Ballota saxatilis subsp. brachyodonta (Boiss.) P. H.Davis & Doroszenko
Ballota schimperi Benth.
Otostegia fruticosa subsp. schimperi (Benth.) Sebald
Ballota sechmenii Gemici & Leblebici
Ballota undulata (Sieber ex Fresen.) Benth.
Otostegia fruticosa (Forssk.) Schweinf. ex Penzig
Otostegia fruticosa subsp. schimperi (Benth.) Sebald
Otostegia fruticosa subsp. schimperi (Benth.) Sebald
Otostegia integrifolia Benth.
Rydingia integrifolia (Benth.) Scheen & V. A.Albert
Otostegia limbata (Benth.) Boiss.
Rydingia limbata (Benth.) Scheen & V. A.Albert ; Ballota limbata Benth.
Otostegia persica (Burm.f.) Boiss.
Rydingia persica (Burm.f.) Scheen & V. A.Albert ; Ballota persica (Brum.f.) Benth
Otostegia tomentosa A.Rich.
Traditional Uses
Several plant species belonging to Ballota and Otostegia genera have been used in traditional medicine of many countries. A summary of their traditional use is presented in [Table 2 ].
Table 2 Ethnopharmacological uses of Ballota and Otostegia taxa.
Species
Vernacular names
Area
Use
Ref
B. acetabulosa
boz ot
Aydin, Turkey
stomach ailments, abdominal pain
[11 ]
Balikesir, Turkey
hemorrhoid treatment
[12 ]
B. africana
kattekruid, oulap
Namaqualand, South Africa
stomach ache, headache, backache, wounds, pediatric, coughs and bronchitis, chest ailments, toothache, burning feet, earache, convulsions, weaning, chilblained hands and feet, mastitis
[13 ]
kattekruid
South Africa
fever, cough, asthma, lung infections, influenza, insomnia, stress
[14 ], [15 ]
B. arabica
kubo, goma
Baluchistan, Pakistan
abortifacient, astringent, stimulant, hemostatic, anthelmintic, diuretic
[16 ]
B. aucheri
golder
Baluchistan, Iran
hair tonic, strengthening gums, dental cleaning and brightness, prevention of hair loss
[17 ]
chashing
Gilgit Baltistan, Pakistan
hair tonic, dental cleaning
[18 ]
B. cinerea
kori
Himachal Pradesh, India
stomachache, analgesic
[19 ]
karui
Uttarakhand, India
fever, jaundice, skin disease, malaria and most prominently in diabetes
[20 ], [21 ]
karui, titpati, patkarru
Nepal, Kashmir
fever, jaundice, scabs, skin disease, malaria, insect repellent
[22 ]
B. deserti
telheret, meriout
Central Sahara
respiratory diseases, fever, colics, colds, cough, digestive troubles, helminthiasis, nausea
[23 ], [24 ]
Tunisia
asthma, diabetes, diuretic
[25 ]
B. hirsuta
uarimsa, touganʼif-zi, tifziguiyin
High Atlas, Morocco
general health, gastrointestinal, gynecological, pediatric
[26 ]
West Algeria
contusion, injuries and rheumatic pain
[27 ]
B. lanata
Mongolia
treatment of pelvic inflammation and chronic pelvic inflammation, edema, irregular menstruation, dysmenorrheal, amenorrhea, nephritis
[28 ]
gang gaʼ chung
Tibet
stomach, intestinal, and gynecological diseases
[29 ]
B. nigra
Sharr Mt., Macedonia
digestive
[30 ]
Moldova
sedative, antispasmodic stimulant, vermifuge
[31 ]
crna kopriva
Northeast Bosnia-Herzegovina
nervous system disorders, sedation
[32 ]
erbo moro
Lucca, Italy
against wounds and sprains
[33 ]
Mediterranean Area
skin disorders, sore throat in horses
[34 ]
bar qene
Albanians, North Basilicata, Italy
diuretic, hemostatic
[35 ], [36 ]
crna kopriva
Bosnia-Herzegovina
hysteria
[37 ]
Jadovnik Mt., Serbia
remedy for upset stomach, nausea, and vomiting; symptomatic, treatment of nervous disorders, sleep disorders, coughs, inflammation, gout
[38 ]
malrubio negro
North Spain
insecticides and repellents against fleas
[39 ]
B. nigra L. subsp. anatolica
leylimkara
Mersin, Turkey
antiseptic for wounds, to treat inflamed sore in armpit or foot
[40 ]
elkurtaran
Taurus Mt., Turkey
to treat flatulence and stomach upset
[41 ]
pemberenkli, oğul otu, arı oto
Gönen, Turkey
burns, wounds, headache
[42 ]
grip otu
Kırklareli, Turkey
cold, flu
[43 ]
O. fruticosa
Yemen
anti-paralytic and for eye diseases
[44 ], [45 ]
North Ethiopia
diarrhea
[46 ]
sasa
Tigray, Ethiopia
repellent of mosquitos
[47 ]
geram tungut
Central Ethiopia
tonsillitis
[48 ]
shakab, sharm
Saudi Arabia
remedy for sun-stroke
[49 ]
O. integrifolia
cheindog
Eritrea
against fleas and mosquitos
[50 ]
tinjute
Ethiopia
stomach-ache, evil eye, fever
[51 ]
tinjute
Ethiopia
repellent of mosquito and house fly, antimalarial
[52 ], [53 ]
tinjute
Ethiopia
Type 2 Diabetes Mellitus
[54 ]
chiendog
Tigray, Ethiopia
ectoparasites in livestock
[55 ]
chiendog
Tigray, Ethiopia
repellent of mosquitos
[47 ]
tungut
Central Ethiopia
evil eye
[48 ]
North Ethiopia
vomiting, nausea, diarrhea, dysentery
[46 ]
North Ethiopia
sterilization, ritual custom
[56 ]
O. limbata
bui, phut kanda
Northwest Pakistan
treatment of childrenʼs gums and for ophthalmia in men, boils, wound, scabies
[57 ], [58 ], [59 ]
pishkand
Battagram, Pakistan
jaundice
[60 ], [61 ]
spin azghay
Malakand, Pakistan
dental problems, wounds, cuts, narcotic, tonic, anticancer and goiter
[62 ]
bui
Punjab, Pakistan
treatment of childrenʼs gums and for opthalmia in man
[63 ]
sassa
Chon. Karak, Pakistan
treatment of childrenʼs gums and for opthalmia in man
[61 ]
chitta jand
Jhelum, Pakistan
acidity
[64 ]
chittakanda
Azad Jammu and Kashmir, Pakistan
antiseptic, antibacterial, wound healing, ophthalmia, gum diseases
[65 ]
spin azghay
Dir lower, Pakistan
hypertension
[66 ]
jand
Azad Kashmir, Pakistan
used to improve eye vision
[67 ]
Abottabad, Cherat, Mardan, Malakand, Kohat, Pakistan
antiulcer, antispasmodic, antidepressant, opthalmia and gums diseases
[68 ]
koribooti
Himalaya
wound healing
[69 ]
O. persica
golder
Baluchistan, Iran
diabetes, rheumatism, cardiac distress, reducing palpitation, hypertension, laxative, carminative, antipyretic, cold, hyper lipidemia, gastric discomfort, parasite repellent, sedative, headache
[17 ]
O. tomentosa
North Ethiopia
ascariasis, diarrhea
[46 ]
In Europe, the most utilized is, by far, B. nigra , a perennial herb native to the Mediterranean region and to central Asia, which can be found throughout Europe. It is also naturalized in Argentina, New Zealand, and the eastern United States. Leaves of B. nigra were used as an antidote for rabid dog bites. It was used in the Balkanic area as a sedative/tranquilizer in cases of hysteria and hypochondria [31 ], [32 ], [37 ], [38 ]. It is also used in Italy externally, for wound-healing properties [33 ], [36 ]. Internally, in the Balkans, it is used as a sedative, a spasmolytic for stomach cramps and aches, for whooping cough, and to increase bile flow. It is also used to treat nervousness, upset stomach, nausea, and vomiting [30 ], [38 ]. In Moldova, in the form of enemas and suppositories, it is used against worm infestation [31 ]. In northern Spain, it is used as insecticide and repellent against fleas [39 ]. In several parts of Turkey, its subspecies B. nigra L. subsp. anatolica , where it is known by different vernacular names, has been reported for the treatment of cold and flu [43 ], flatulence, and upset stomach [41 ] and as antiseptic for wounds, burns, and inflamed skin [40 ], [42 ]. B. acetabulosa , known as the Greek horehound, is a compact, evergreen subshrub, growing to 0.5 m, native to southeast Greece, Crete, and western Turkey. In Turkey, the infusion of leaves is used for treatment of stomach ailments, where the leave poultice relieves abdominal pain and hemorrhoids [11 ], [12 ].
In the southern part of Africa, the only species present is B. africana , known as Cape horehound or “kattekruie.” It is most common in the more arid, winter rainfall areas of the Cape. Its natural distribution stretches from the southern part of Namibia down to the West Coast and Cape Peninsula. Along this wide distribution, B. africana is usually found along streams and in the shelter of rocks and bushes. Externally, a leaf compress is applied on sick childrenʼs feet, on painful legs, inflamed joints, backache, head for headache, on cheek for toothache, on breasts for mastitis, wash for chilblained hands and feet, wounds, ointment on sores, and as poultice on boils. Orally, leaf infusion is used for stomach ache, influenza, fever, asthma, lung, and urinary infections, to treat convulsions in infants, to wean infants, and as cough syrup [13 ], [14 ], [15 ].
Ballota deserti (syn. Marrubium deserti ) is a common endemic species in the northern and central Sahara. In Tunisia, it is employed in traditional medicine in the form of a decoction as a remedy for asthma, diabetes, and as a diuretic [25 ]. The internal usage of this species has been documented in the central Sahara using the infusion of its leaves for respiratory diseases, fever, colic, colds, cough, digestive troubles, helminthiasis, and nausea [23 ], [24 ]. Another plant utilized in North Africa (High Atlas, Morocco, and West Algeria) is B. hirsuta , native to the western Mediterranean region, mostly abundant in Spain, Portugal, and North Africa. It is very popular with traditional healers (known as “uarimsa,” “touganʼif-zi,” or “tifziguiyin”) as a cure for many diseases. The poultice of leaves and roots is used very often to treat subcutaneous lesions (contusion), rheumatic pains, and heal various wounds. The decoction of flowers is used externally as an antiseptic or orally against dental caries, whereas the flower infusion is utilized internally to treat gastrointestinal, gynecological and pediatric diseases [26 ], [27 ].
In East Africa (Eritrea, Ethiopia), there are many reports of some Otostegia species concerning their usage in traditional medicine. The most numerous data are available for O. integrifolia (syn. R. integrifolia ). O. integrifolia , commonly known as Abyssinian rose, is endemic to Ethiopia, where it is known as “tinjute” (ጥንጁት), growing in the dry evergreen woodlands regions at altitudes of 1300 – 2800 m above sea level. It also grows in Eritrea and Yemen. In northern Ethiopia, it is commonly used to smoke utensils for sterilization. It is also a ritual custom for a mother to cleanse herself with the smoke on the tenth day after giving birth to a child before leaving her confinement to resume normal daily activities [56 ]. It has been largely employed as an insect repellent against fleas and mosquitos and as antimalarial [47 ], [50 ], [52 ], [53 ]. Inhalation of the smoke of burnt stems and leaves is used against evil eye [48 ], [51 ] and its juice, diluted with water, is drunk for treatment of stomach ache, vomiting, nausea, diarrhea, and dysentery [46 ], [51 ]. In the same geographical area, the juice of O. tomentosa and O. fruticosa has been similarly used against diarrhea [46 ], and the latter also against ascariasis [46 ] and tonsillitis [48 ].
O. fruticosa also grows in the Arabic peninsula where the infusion of flowering branches is used as a remedy for sun-stroke [49 ] or as an anti-paralytic and for eye diseases [44 ], [45 ].
In Pakistan, India and Iran, species belonging to Ballota and Otostegia genera have been largely employed in traditional medicine.
B. arabica (syn. Leucas urticifolia (Vahl) Sm.) is an annual herb distributed in the Punjab, Baluchistan, Sindh, and Rajputana desert of Pakistan. In Baluchistan, where it is known as “kubo” or “goma,” the plant is used as a cure for fever. Furthermore, the decoction of the leaves and apical shoots is used as an abortifacient up to 3 mo of pregnancy. Infusions of the flowers are used to treat skin diseases. The plant is also used for the treatment of diarrhea, dysentery, uterine hemorrhages, dropsy, gravel, cystitis, calculus, bronchial catarrh, skin diseases, fever, and various types of mental disorders [16 ]. The decoction of leaves, roots and flowers of B. aucheri is topically employed in both Pakistan and Iran as hair tonic, for strengthening gums, dental cleaning and brightness, and prevention of hair loss [17 ], [18 ]. While in Baluchistan, Iran, the decoction of leaves and flowers of O. persica (“golder”) is drunk for treatment of diabetes, rheumatism, cardiac distress, palpitation, hypertension, cold, hyper lipidemia, gastric discomfort, headache, and as parasite repellent, sedative, laxative, carminative, and antipyretic [17 ]. In Pakistan, the largest number of ethno-pharmacological reports involve O. limbata . In fact, it is extensively utilized by traditional practitioners against several ailments since it possesses antispasmodic, antiulcer, antidepressant, sedative, and anxiolytic properties [68 ]. O. limbata is consumed for the treatment of childrenʼs gum problems and for remedial purposes in cases of ophthalmia [57 ], [58 ], [59 ], [61 ], [63 ]. Local, fresh leaves of O. limbata are crushed and then grounded and mixed with water to make the extract which is also used to cure eye infections. Due to its antiseptic and antibacterial properties [65 ], powder of dried leaves is mixed with butter and layered on wounds and boils in both humans and animals [59 ], [65 ], [69 ]. Dried plant powder is also utilized against jaundice [61 ], [63 ].
Ballota cinerea D. Don is vernacularly known as Karui, Titpatti, or Patkarru. WP parts are widely used as folk medicine in India and Nepal. Shoots are crushed and eaten with salt to strengthen the liver by local villagers. Young shoots are used as insect repellent for cattle during rainy season. Leaves and shoot extraction are used in scabs and other skin infections. AP are widely used to treat malaria and various liver disorders like jaundice, liver debility, and fever [20 ], [21 ].
The traditional uses of Ballota and Otostegia species are wide and sometimes may be directly correlated to the content of some active class of compounds. Along with diterpenes that characterize these species, flavonoids and phenolic compounds, the latter ones often occurring as esters moieties, are the main constituents of the plant extracts and their antibacterial and anti-inflammatory activities are well documented in literature. They could be responsible for most of the claimed remedies. In the following sections the metabolic profiles and the biological activities of these plants have been analyzed.
Phytochemicals
Diterpenoids
Seventy-five diterpenes ([Fig. 1 ], [Fig. 2 ], [Fig. 3 ]) were isolated and characterized both by their AP and roots of taxa of genus Ballota and Otostegia, and their presence is summarized in [Table 3 ] (labdane diterpenoids) and [Table 4 ] (other diterpenoids). Apart from 7α -acetoxyroyleanone (73 ) and coleon A (74 ), belonging to abietane diterpenoids, and 7,8β -epoxymomilactone-A (75 ), belonging to pimarane diterpenoids, 3 main carbon-skeletons occur: labdane, hispanane, and clerodane.
Fig. 1 Structures of ladbane diterpenes.
Fig. 2 Structures of ladbane diterpenes.
Fig. 3 Structures of hispanane, clerodane, abietane, and pimarane diterpenes.
Table 3 Distribution of labdane diterpenes in Ballota and Otostegia taxa.
No
Names
Taxa
1
7α -acetoxymarrubiin
B. nigra
[70 ]
2
6-acetyl-marrubenol
B. deserti
[24 ]
3
19-acetyl-marrubenol
B. deserti
[24 ]
4
balloaucherolide
B. aucheri
[71 ]
5
ballonigrin
B. acetabulosa, B. antalyensis, B. cristata, B. larendana, B. saxatilis subsp. brachyodonta
[72 ], [73 ], B. aucheri
[74 ], B. inaequidens
[72 ], [73 ], [75 ], [76 ], B. lanata
[77 ], B. nigra
[70 ], B. nigra subsp. foetida
[72 ], [73 ], [78 ], B. pseudodictamnus
[79 ], B. rupestris
[70 ], [78 ], B. saxatilis
[72 ], [73 ], [80 ], B. undulata
[81 ], O. fruticosa
[82 ]
6
ballonigrin lactone A
O. limbata
[83 ]
7
ballonigrin lactone B
O. limbata
[83 ]
8
ballonigrinone
B. rupestris
[70 ], [78 ], B. undulata
[81 ]
9
ballotenol
B. nigra subsp. foetida
[84 ]
10
ballotinone
B. aucheri
[71 ], B. nigra subsp. foetida
[85 ], B. undulata
[81 ]
11
calyenone
B. cinerea
[86 ]
12
calyone
B. cinerea
[86 ], [87 ]
13
cinereanoid A
B. cinerea
[87 ]
14
cinereanoid B
B. cinerea
[87 ]
15
cinereanoid C
B. cinerea
[88 ]
16
cinereanoid D
B. cinerea
[88 ]
17
cyllenin A
B. deserti
[89 ], [90 ]
18
dehydrohispanolone (hispanone)
B. acetabulosa, B. antalyensis, B. cristata, B. larendana, B. latibracteolata, B macrodonta, B. nigra subsp. uncinata, B. pseudodictamnus subsp. lycia, B. rotundifolia, B. saxatilis subsp. brachyodonta
[72 ], [73 ], B. saxatilis
[72 ], [73 ], [80 ], B. undulata
[81 ], O. fruticosa
[82 ]
19
6-dehydroxy-19-acetyl-marrubenol
B. deserti
[24 ]
20
desertin
B. deserti
[89 ], [90 ]
21
15-epi -cyllenin A
B. deserti
[89 ], [90 ]
22
15-epi -leopersin C
O. fruticosa
[82 ]
23
15-epi -otostegin B
O. fruticosa
[82 ]
24
16-epoxy-9-hydroxylabda-13(16),14-diene
B. deserti
[24 ]
25
hispanolone
B. acetabulosa, B. cristata, B. pseudodictamnus subsp. lycia, B. rotundifolia, B. saxatilis subsp. brachyodonta
[72 ], [73 ], B. africana
[91 ], B. andreuzziana
[79 ], B. hirsuta
[92 ], B. inaequidens
[72 ], [73 ], [75 ], [76 ], B. saxatilis
[72 ], [73 ], [80 ]
26
3β -hydroxyballotinone
B. undulata
[81 ]
27
6β -hydroxy-15,16-epoxy-labda-8,13(16),14-trien-7-one
B. aucheri
[74 ]
28
6β -hydroxy-15α -methoxy-9α ,13,15,16-bis-epoxylabd-7-one
B. aucheri
[71 ]
29
6β -hydroxy-15β -methoxy-9α ,13,15,16-bis-epoxylabd-7-one
B. aucheri
[71 ]
30
6β -hydroxy-15β -ethoxy-9α ,13,15,16-bis-epoxylabd-7-one
B. aucheri
[71 ]
31
9α -hydroxy-6,9 : 15,16-diepoxy-13(16),14-labdadien-7-one
B. aucheri
[74 ]
32
13-hydroxyballonigrolide
B. lanata
[77 ], B. nigra
[93 ], [94 ]
33
18-hydroxyballonigrin
B. acetabulosa
[95 ], B. pseudodictamnus
[79 ], B. saxatilis
[96 ]
34
leoheterin
B. aucheri
[71 ], [74 ], [97 ], O. fruticosa
[82 ], [98 ]
35
leopersin C
O. fruticosa
[82 ]
36
marrubenol
B. pseudodictamnus
[79 ]
37
marrubiin
B. deserti
[89 ], [90 ], B. nigra subsp. foetida
[85 ], [99 ]
38
marrulactone
B. deserti
[89 ], [90 ]
39
marrulibacétal
B. deserti
[89 ], [90 ]
40
marrulibacétal A
B. deserti
[90 ]
41
otostegin A
O. fruticosa
[82 ]
42
otostegin B
O. fruticosa
[82 ]
43
otostegindiol
O. integrifolia
[100 ], [101 ]
44
persianone
B. aucheri
[74 ]
45
precalyone
B. cinerea
[86 ]
46
preleoheterin
B. aucheri
[71 ], [97 ], O. fruticosa
[82 ]
47
preleosibirin
B. nigra subsp. foetida
[102 ]
48
preotostegindiol
O. integrifolia
[100 ]
49
rupestralic acid
B. rupestris
[103 ]
50
vulgarol
O. fruticosa
[82 ]
Table 4 Distribution of other diterpenes in Ballota and Otostegia taxa.
Hispanane skeleton
51
hispaninic acida
B. hispanica
[104 ], [105 ]
52
hispanonic acida
B. hispanica
[104 ], [105 ]
53
limbetazulone
O. limbata
[106 ]
54
limbatenolide A
O. limbata
[107 ]
55
limbatenolide B
O. limbata
[107 ]
56
limbatenolide C
O. limbata
[107 ], O. persica
[108 ]
57
limbatenolide D
O. limbata
[109 ]
58
limbatenolide E
O. limbata
[109 ]
Clerodane skeleton
59
ballatenolide A
O. limbata
[107 ], O. persica
[108 ]
60
ballotenic acid
O. limbata
[110 ]
61
ballotenic acid A
O. limbata
[111 ]
62
ballodiolic acid
O. limbata
[110 ]
63
ballodiolic acid A
O. limbata
[111 ]
64
limbatolide A
O. limbata
[112 ]
65
limbatolide B
O. limbata
[112 ]
66
limbatolide C
O. limbata
[112 ]
67
limbatolide D
O. limbata
[113 ]
68
limbatolide E
O. limbata
[113 ]
69
limbatolide F
O. limbata
[114 ]
70
limbatolide G
O. limbata
[114 ]
71
15-methoxypatagonic acid
O. limbata
[107 ], O. persica
[108 ]
72
patagonic acid
O. limbata
[107 ], O. persica
[108 ]
Abietane skeleton
73
7α -acetoxyroyleanone
B. nigra
[115 ]
74
coleon A
B. cinerea
[116 ]
Pimarane skeleton
75
7,8β -epoxymomilactone-A
B. arabica
[117 ]
The labdane diterpenoids (1 –50 ) ([Figs. 1 ] and [2 ]) are characterized by some interesting structural features. They all belong to a normal labdane stereochemical series, although Gray et al. [118 ] claimed, based on the optical rotation, that compound 27 had an ent -labdane skeleton. The C-11-C-16 fragment, never carrying an oxygenated function on C-11 and C-12, can occur with different substructures. The most common one involves C-13/C-16 in a furane ring that, in a few cases, is oxidized to γ -lactone (6, 7, 13 –16, 32, 49 ). In almost all of the remaining labdanes, C-13 is involved in the formation of a spiro structure including C-9. By NOE correlations between H-16 and Me-17, it has been shown that all of them belong to the 13R stereochemical series.
The decalin moiety contains some constant functional features: the decalin junction is always trans and the methyl groups (C-17) in position C-8; when it is not present, a C-8/C-9 double bond is always α -orientated. In the majority of the structures, C-6 and C-7 show oxygenated functions and methyl 18 is devoid of functionalization.
Hispanane-type diterpenoids ([Fig. 3 ]) are a scarce group of natural diterpenoids that exhibits a 6/7/6 tricylic system featured with a 7-membered carbon ring.
To our knowledge, apart from hispaninic acid (51 ) and hispanonic acid (52 ), isolated from B. hispanica
[104 ], [105 ], limbatenolides A (54 ), B (55 ), D (57 ), E (58 ) [107 ], [109 ], and limbetazulone (53 ) [106 ], isolated from O. limbata and limbatenolide C (56 ), isolated both from O. limbata
[107 ] and O. persica
[108 ], only 5 other natural hispananes have been characterized up until now: methyl verticoate (from Sciadopitys verticillata (Thunb.) Seibold & Zucc.) [119 ], salviatalin A [120 ], salviadigitoside A [120 ] and salviatalin A 19-O -β -glucoside [121 ] (from Salvia digitaloides Diels), and viburnumoside (from Viburnum cylindricum Buchanan-Hamilton ex D. Don) [122 ].
With the exception of viburnumoside, shown to have an ent absolute configuration (β -H 5 and α -CH3 20) [122 ], the absolute configurations of any of the other hispananes have been not determined. In this review, we will report the configuration depicted in the original papers (α -H 5 and β -CH3 20). The plausible biosynthetic pathway was speculated as a pimarane [121 ] or labdane way [123 ].
Clerodane diterpenods ([Fig. 3 ]) showed, differently from labdanes, both a trans junction and a cis junction of the decalin moiety (64 –66 ). Apart from ballodiolic acid (62 ) and ballodiolic acid A (63 ), all the other compounds showed the C-13/C-16 as furane ring (67, 68 ) or γ -lactone. Common features to all compounds are the absence of functionalizations at C-1, C-2, C-7, C-11, C-12, Me-17, Me-19, and Me-20 and the presence of a carboxylic acid on C-18 that, in some cases, is lactonized with the hydroxyl group on C-6 (59, 61, 63, 64, 67 –70 ). In [Table 5 ], all of the diterpenes are listed, according to their skeleton, in alphabetical order along with their 13 C NMR spectra, when available.
Table 5 13 CNMR data of diterpenes from Ballota and Otostegia taxa.
Skeleton numbering
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
1′
2′
Ref.
a The spectra were recorded as methyl ester derivative.
Labdane skeleton
1
7α -acetoxymarrubiin
33.4
17.5
29.3
43.7
46.0
75.8
70.6
38.8
79.2
39.1
28.4
18.4
124.6
110.7
138.8
143.2
13.9
22.8
183.1
22.6
171.0
21.0
[70 ]
2
6-acetyl-marrubenol
Not reported in literature
3
19-acetyl-marrubenol
Not reported in literature
4
balloaucherolide
37.2
17.2
32.3
35.7
124.3
143.1
181.7
140.5
165.8
43.8
23.8
29.4
127.4
110.5
140.5
143.1
11.6
28.1
27.9
27.6
[71 ]
5
ballonigrin
30.3
17.8
27.7
42.0
49.5
75.5
193.2
131.2
166.7
36.7
30.0
24.6
123.7
110.5
143.1
138.8
11.9
24.5
180.1
28.0
[81 ]
6
ballonigrin lactone A
28.1
21.9
27.6
42.2
49.3
78.8
194.1
135.7
165.3
39.1
29.9
24.6
139.1
141.3
100.9
170.6
17.4
26.7
176.4
22.4
56.2
[83 ]
7
ballonigrin lactone B
26.1
21.3
28.2
45.9
48.3
80.1
197.1
132.7
162.4
38.5
31.2
22.9
136.5
145.9
74.4
172.1
17.6
27.1
178.5
23.1
[83 ]
8
ballonigrinone
30.1
34.0
203.4
52.1
50.2
74.5
191.3
132.6
163.7
36.1
30.3
24.1
123.4
110.5
143.3
138.8
12.1
21.6
172.1
24.2
[81 ]
9
ballotenol
35.5
18.9
39.3
44.2
51.4
74.9
213.0
46.0
82.9
40.1
43.5
22.0
126.0
111.4
143.3
138.8
8.7
27.8
67.0
20.1
[84 ]
10
ballotinone
28.8
17.8
28.8
41.6
44.6
75.6
209.4
51.4
77.7
40.6
37.8
18.1
124.2
110.6
143.1
138.7
15.7
26.3
180.3
17.9
[81 ]
11
calyenone
34.5
22.9
76.8
36.6
44.5
30.1
199.3
166.1
130.6
40.4
29.4
24.3
124,3
110.5
142.3
138.6
18.0
27.0
21.3
11.5
170.2
21.1
[86 ]
12
calyone
25.6
22.7
77.3
37.0
41.1
38.5
211.4
51.0
81.6
43.0
34.7
21.5
124.6
110.6
143.1
138.5
8.3
27.6
21.2
16.0
170.6
21.5
[87 ]
13
cinereanoid A
30.4
23.8
78.5
37.7
46.2
35.5
201.9
131.9
168.3
42.1
27.8
27.6
176.3
118.2
173.6
100.9
11.6
27.6
21.7
18.2
172.3
21.1
[87 ]
14
cinereanoid B
26.8
23.8
79.2
38.1
42.3
39.5
214.5
51.9
82.5
44.5
32.3
25.6
172.6
117.8
173.7
101.2
8.6
28.2
21.9
16.8
172.6
21.1
[87 ]
15
cinereanoid C
30.4
23.8
78.5
37.7
46.2
35.5
202.0
132.0
168.5
42.1
25.6
28.5
137.4
147.0
99.2
173.8
11.8
27.6
21.8
18.2
172.4
21.1
[88 ]
16
cinereanoid D
35.5
69.2
77.6
38.9
45.6
35.2
201.3
132.3
166.8
43.0
27.5
27.5
170.9
118.4
173.4
100.9
11.7
27.8
21.4
19.4
172.1
21.1
[88 ]
17
cyllenin A
28.4
17.3
27.6
43.6
45.8
75.7
30.9
31.2
91.3
38.5
29.1
34.7
89.5
46.0
98.7
76.1
16.9
22.6
183.6
23.0
[124 ]
18
dehydrohispanolone (hispanone)
35.8
18.6
41.3
33.1
50.2
35.2
200.3
130.3
167.0
40.9
30.2
24.2
124.5
110.5
143.0
138.6
11.4
32.5
21.3
18.1
[125 ]
19
6-dehydroxy-19-acetyl-marrubenol
31.6
18.2
35.7
36.9
47.5
22.6
31.2
36.6
79.6
43.0
35.0
22.6
125.5
110.8
142.8
138.4
16.2
26.8
67.1
16.9
171.4
20.9
[24 ]
20
desertin
28.4
18.2
28.3
43.9
45.0
76.3
31.6
32.5
75.3
40.1
28.2
27.4
81.2
80.2
110.8
108.6
16.8
23.0
183.9
22.2
56.4
55.1
[89 ]
21
15-epi -cyllenin A
28.3
17.5
27.7
43.6
45.8
75.7
30.9
31.2
90.0
38.4
28.9
37.2
89.5
48.1
98.7
76.4
16.9
22.4
183.3
23.2
[124 ]
22
15-epi -leopersin C
32.7
18.3
42.4
32.4
57.2
212.0
77.4
47.0
93.4
48.3
29.5
38.8
91.0
47.9
99.0
78.4
13.3
22.2
32.4
19.7
[126 ]
23
15-epi -otostegin B
34.8
19.1
44.1
35.2
49.1
76.0
204.5
45.5
98.2
43.9
30.1
38.8
90.6
42.1
101.2
71.0
9.5
33.1
24.9
20.3
169.8
21.8
[82 ]
24
16-epoxy-9-hydroxylabda-13(16),14-diene
Not reported in literature
25
hispanolone
31.9
18.5
41.3
33.6
46.4
39.2
211.9
50.9
81.8
43.3
34.7
21.5
124.8
110.7
143.0
138.6
8.2
33.1
21.4
16.2
[127 ]
26
3β-hydroxyballotinone
27.8
28.2
73.5
44.1
49.9
75.9
208.3
51.6
77.5
40.8
37.8
18.0
124.0
110.5
143.3
138.8
15.8
23.2
180.0
17.7
[81 ]
27
6β-hydroxy-15,16-epoxy-labda-8,13(16),14-trien-7-one
37.4
18.6
43.2
33.9
53.2
70.7
199.4
128.2
169.8
41.1
30.6
24.2
124.3
110.4
142.9
138.5
11.6
32.4
23.9
18.6
[74 ]
28
6β-hydroxy-15α -methoxy-9α ,13,15,16-bis-epoxylabd-7-one
Not reported in literature
29
6β-hydroxy-15β-methoxy-9α ,13,15,16-bis-epoxylabd-7-one
Not reported in literature
30
6β-hydroxy-15β-ethoxy-9α ,13,15,16-bis-epoxylabd-7-one
Not reported in literature
31
9α -hydroxy-6,9 : 15,16-diepoxy-13(16),14-labdadien-7-one
31.8
19.3
41.3
32.3
55.6
81.8
214.8
32.4
108.0
48.9
36.0
18.7
124.8
110.8
142.8
138.6
7.5
33.8
22.0
16.8
[74 ]
32
13-hydroxyballonigrolide
30.2
18.1
28.1
42.1
49.3
76.1
193.6
131.1
167.1
37.0
24.3
37.5
76.4
42.5
180.2
79.1
24.5
27.9
180.9
12.1
[77 ]
33
18-hydroxyballonigrin
30.2
17.7
22.5
48.8
45.6
77.3
193.5
131.2
166.6
36.4
29.4
24.2
123.8
110.6
143.2
138.8
29.0
67.5
180.1
12.1
[95 ]
34
leoheterin
31.6
18.1
42.0
32.2
55.9
212.0
77.1
47.6
77.2
49.0
34.2
21.2
124.7
110.6
143.2
138.6
12.4
32.6
22.2
18.0
[74 ]
35
leopersin C
32.3
18.2
42.4
32.4
57.0
211.5
77.4
46.8
92.1
48.2
29.1
35.9
90.7
46.4
99.0
76.9
13.1
22.1
32.4
19.7
[126 ]
36
marrubenol
33.8
18.5
40.7
38.9
49.3
65.9
38.9
31.1
77.0
43.4
34.9
21.5
125.4
110.8
142.8
138.5
16.2
27.8
69.1
19.6
[128 ]
37
marrubiin
35.1
18.1
28.5
43.8
44.8
76.3
31.4
32.3
75.6
39.7
28.3
21.0
125.1
110.7
142.9
138.5
16.6
22.9
184.0
22.3
[85 ]
38
marrulactone
28.8
18.4
28.5
44.1
45.0
75.7
31.7
32.4
88.3
39.0
34.5
20.5
34.4
172.1
16.8
23.1
183.4
22.6
[129 ]
39
marrulibacétal
27.9
17.9
28.2
43.9
44.7
76.5
32.3
33.7
80.4
41.0
21.1
29.6
75.6
78.5
108.7
105.4
19.5
23.2
183.9
22.0
63.9
15.0
[129 ]
40
marrulibacétal A
27.8
17.9
28.2
43.9
44.6
76.6
32.3
33.5
80.3
40.9
20.7
30.0
75.8
78.6
109.8
105.7
19.4
23.2
184.1
22.0
55.5
[90 ]
41
otostegin A
34.4
28.7
43.7
34.9
50.2
77.1
204.2
46.7
96.6
43.1
30.4
37.6
93.7
106.9
148.2
80.5
9.2
32.6
23.9
29.4
269.4
21.3
[82 ]
42
otostegin B
34.9
19.1
44.3
35.2
49.3
76.1
204.5
45.8
98.1
43.8
30.4
38.9
90.7
42.2
101.2
71.0
9.5
33.1
24.2
20.3
169.8
21.8
[82 ]
43
otostegindiol
25.1
25.6
76.3
43.2
39.8
21.6
31.4
37.1
77.5
37.8
35.5
21.9
126.1
111.3
143.2
138.9
16.5
22.6
29.0
16.8
[130 ]
44
persianone
31.5
18.2
42.3
32.3
57.4
210.7
65.0
50.5
77.0
48.7
43.6
21,5
124.9
110.7
143.0
138.6
13.1
32.7
22.3
18.0
[74 ]
36.5
18.7
42.2
32.1
58.3
211.3
67.9
128.1
144.4
45.1
29.2
25.4
125.1
110.7
142.8
138.5
16.5
33.1
21.9
21.3
45
precalyone
38.2
22.7
77.5
36.9
40.1
38,2
210.2
50.0
86.0
42.5
29.4
26.4
94.0
107.0
148.3
80.8
17.1
27.1
21.2
9.2
170.3
21.1
[86 ]
46
preleoheterin
42.2
18.3
32.3
32.4
57.1
212.1
77.2
47.5
93.9
48.1
29.9
37.7
92.1
107.3
148.3
80.9
13.2
32.3
22.3
19.3
[71 ]
47
preleosibirin
Not reported in literature
48
preotostegindiol
25.1
25.2
76.1
42.4
39.8
21.2
31.6
37.3
93.3
37.7
32.0
35.6
93.0
107.6
147.8
81.5
17.6
22.4
29.5
21.1
[130 ]
49
rupestralic acid
30.1
18.1
28.1
42.3
49.4
76.1
193.8
131.7
165.6
36.9
24.7
27.3
136.0
146.5
98.5
172.1
24.7
27.4
180.7
12.1
[77 ]
50
vulgarol
36.8
19.0
42.6
33.3
46.9
21.0
37.8
74.0
61.2
39.2
25.8
43.4
140.9
123.4
59.5
16.9
32.3
33.5
21.7
25.2
[82 ]
Hispanane skeleton
51
hispaninic acida
36.7
19.4
37.4
43.8
52.9
20.7
34.1
126.2
147.9
40.8
25.0
27.0
151.2
113.6
169.4
158.1
116.5
28.3
177.4
16.9
51.2
[131 ]
52
hispanonic acida
36.3
19.4
37.4
43.8
53.0
20.1
28.1
134.7
154.0
41.3
27.6
25.0
135.7
112.7
145.8
149.6
183.1
28.2
177.7
16.7
51.2
[131 ]
53
limbetazulone
34.3
27.5
80.1
42.8
52.2
18.2
27.9
134.3
154.3
40.2
25.1
28.0
135.8
112.8
146.0
149.6
183.1
22.5
64.1
29.9
[106 ]
54
limbatenolide A
34.2
18.1
27.5
42.8
51.1
80.1
28.0
134.2
154.1
40.1
25.0
27.9
135.6
112.7
145.9
149.1
183.0
22.5
64.1
19.9
[107 ]
55
limbatenolide B
34.5
18.6
29.6
42.7
51.1
80.1
33.8
125.7
147.9
39.0
24.7
27.0
151.0
113.7
172.0
157.9
116.1
22.4
64.0
20.2
[107 ]
56
limbatenolide C
37.2
19.5
36.0
43.5
53.0
20.8
35.6
127.5
142.4
40.5
25.2
27.8
130.1
115.1
172.9
143.2
114.9
28.1
182.2
17.2
[107 ]
57
limbatenolide D
38.4
20.4
32.4
44.1
53.1
83.5
29.2
136.1
152.6
42.3
25.4
28.6
135.9
113.4
144.1
150.6
184.3
25,2
180.1
18.5
[109 ]
58
limbatenolide E
37.1
20.9
33.0
42.6
51.9
80.3
35.6
127.1
145.9
40.3
24.8
27.2
250.1
114.1
170.6
156.7
118.2
27.0
181.9
19.3
[109 ]
Clerodane skeleton
59
ballatenolide A
17.5
27.3
130.1
139.7
39.1
86.1
31.4
38.1
42.3
44.9
36.4
19.9
138.8
141.6
102.5
172.0
15.5
171.0
16.1
19.7
57.1
[107 ]
60
ballotenic acid
17.4
27.4
140.5
141.1
37.5
35.7
27.2
36.1
38.7
46.5
36.0
22.6
39.6
29.6
66.4
172.8
15.9
171.0
20.5
18.3
[110 ]
61
ballotenic acid A
17.8
28.1
134.2
140.1
40.1
84.5
30.9
39.3
42.5
45.7
35.9
23.6
41.2
32.3
70.1
173.2
16.5
172.7
17.8
20.6
[111 ]
62
ballodiolic acid
17.5
27.4
140.0
141.3
37.5
35.6
27.2
36.1
38.6
46.6
35.8
24.9
39.8
29.7
66.3
61.1
15.9
172.0
20.5
18.4
[110 ]
63
ballodiolic acid A
18.5
26.4
132.6
138.9
41.1
85.3
32.7
37.5
43.2
46.1
37.3
25.9
40.1
30.5
68.8
63.6
15.5
171.3
16.5
18.1
[111 ]
64
limbatolide A
19.4
28.5
132.1
140.3
40.5
85.2
30.3
37.5
40.9
45.6
37.8
21.3
139.7
142.3
100.8
173.6
15.9
170.8
31.7
22.5
55.7
[112 ]
65
limbatolide B
18.2
28.3
142.1
139.5
38.2
36.1
27.2
37.1
39.9
45.2
35.7
20.3
133.3
142.3
103.4
173.1
16.3
171.8
32.3
23.1
57.6
[112 ]
66
limbatolide C
18.1
27.3
142.3
139.1
38.5
36.0
26.5
38.3
40.1
45.4
36.9
21.3
136.1
144.4
71.4
173.7
16.8
171.5
33.1
16.8
[112 ]
67
limbatolide D
18.2
27.1
132.6
140.5
40.6
82.4
32.6
39.1
41.3
45.1
38.2
18.4
131.5
111.3
143.4
137.7
16.6
174.1
18.1
19.3
[113 ]
68
limbatolide E
30.5
72.3
125.6
139.6
42.3
84.5
33.1
40.5
44.3
46.7
37.1
20.1
130.1
111.7
143.6
140.2
15.8
172.1
19.3
19.8
[113 ]
69
limbatolide F
20.3
27.4
131.1
140.1
43.0
84.4
32.7
37.5
41.6
47.1
36.6
19.6
133.4
143.4
71.4
173.0
15.8
170.4
18.2
19.0
[114 ]
70
limbatolide G
19.5
28.3
133.9
141.2
42.4
83.4
33.8
39.3
40.1
46.2
37.8
18.0
135.6
141.4
101.1
171.8
16.1
169.3
17.5
19.5
[114 ]
71
15-methoxypatagonic acid
17.4
27.2
141.3
140.4
37.5
35.7
27.4
36.3
38.8
46.7
36.3
19.3
134.0
143.0
102.5
172.2
15.5
171.3
20.5
18.1
57.0
[107 ]
72
patagonic acid
17.3
27.4
141.2
140.4
37.5
35.7
27.2
36.2
38.7
46.6
36.2
19.2
134.9
143.5
70.2
174.3
15.9
172.5
20.5
18.2
[107 ]
Abietane skeleton
73
7α -acetoxyroyleanone
35.8
18.8
41.0
33.0
46.1
24.6
64.5
139.4
149.9
39.1
183.7
150.7
124.7
185.4
24.1
19.7
19.9
21.6
33.0
18.5
169.5
21.1
[132 ]
74a
coleon A(19R )
119.5
132.5
43.0
50.4
137.0
152.4
146.9
116.8
120.3
134.4
180.6
154.5
125.4
191.2
23.9
19.8
19.8
18.4
107.1
17.4
[133 ]
74b
coleon A(19S )
118.4
135.1
38.5
50.0
136.9
152.1
146.9
116.8
120.2
134.3
180.6
154.5
125.5
191.2
23.9
19.8
19.8
24.6
110.5
17.6
[133 ]
Pimarane skeleton
75
7,8β -epoxymomilactone-A
Not reported in literature
Flavonoids
In the extensive bibliographic search undertaken, a total of 91 different flavonoids were identified from 22 taxa belonging to Ballota genus and 3 taxa belonging to Otostegia genus.
The structures of the sugar and acyl groups occurring in the secondary metabolites are shown in [Fig. 4 ] and the formula of all compounds are depicted in [Figs. 5 ]–[10 ]. The reported compounds encompass flavones (23 compounds; [Fig. 5 ]), flavonols (13 compounds; [Fig. 6 ]), flavonoid glycosides (24 compounds; [Fig. 7 ]) flavonoid acyl derivatives (20 compounds; [Fig. 8 ]), C -glycosyl-flavonoids (4 compounds; [Fig. 9 ]), flavanones derivatives (4 compounds; [Fig. 10 ]), and flavanols (4 compounds; [Fig. 10 ]).
Fig. 4 Structures of sugars and acyl moieties.
Fig. 5 Structures of flavones.
Fig. 6 Structures of flavonols.
Fig. 7 Structures of glycosyl flavonoids.
Fig. 8 Structures of acyl flavonoids.
Fig. 9 Structures of C-glycosyl flavones.
Fig. 10 Structures of flavanones and flavanols.
[Tables 6 ], [7 ], [8 ] contain all the flavonoids with their semi-systematic or trivial names, and the genera and species, ordered alphabetically, from which the compounds have been isolated. The most common compounds are apigenin-7-O -β -D-glucopyranoside (112 ) (9 taxa), ladanein (79 ) (8 taxa), apigenin (75 ) (6 taxa), luteolin-7-O -β -D-glucopyranoside (116 ) (6 taxa), and rutin (124 ) (6 taxa).
Table 6 Distribution of flavones in Ballota and Otostegia taxa.
No
Name
Taxa
76
apigenin
B. acetabulosa
[75 ], [134 ], [135 ]; B. deserti
[89 ]; B. hirsuta
[136 ], [137 ]; B. lanata
[138 ]; B. nigra
[139 ]; B. pilosa
[140 ]
77
6-methylapigenin
O. persica
[141 ], [142 ]
78
genkwanin
B. hirsuta
[136 ]
79
apigenin 7,4′-dimethyl ether
B. inaequidens
[73 ], [75 ], [76 ]; B. lanata
[143 ], [144 ], [145 ]; B. pseudodictamnus
[79 ]; B. rotundifolia
[73 ]
80
ladanein
B. acetabulosa
[73 ]; B. hirsuta
[136 ]; B. inaequidens
[75 ]; B. latibracteolata
[73 ]; B. nigra
[115 ]; B. rotundifolia
[73 ]; B. saxatilis
[73 ]; B. saxatilis ssp. brachyodonta
[146 ]
81
salvigenin
B. glandulosissima
[75 ], [147 ]; B. hirsuta
[136 ]
82
scutellarein 7,4′-dimethyl ether
B. acetabulosa
[75 ], [134 ]
83
cirsimaritin
B. andreuzziana
[148 ]; B. pilosa
[140 ]
84
pectolinarigenin
O. fruticosa
[149 ]
85
luteolin
B. acetabulosa
[135 ]; B. hirsuta
[136 ]; B. lanata
[138 ]; B. nigra
[139 ]
86
luteolin 7-methyl ether
B. andreuzziana
[148 ]
87
pilloin
B. cinerea
[87 ]
88
luteolin-7,3′,4′-trimethyl ether
B. glandulosissima
[73 ], [75 ], [147 ]; B. inaequidens
[73 ], [75 ], [76 ]; B. lanata
[145 ]
89
nuchensin
B. hirsuta
[136 ]
90
velutin
B. glandulosissima
[73 ], [75 ], [147 ]; B. undulata
[150 ]
91
chrysoeriol
B. lanata
[138 ]; B. nigra
[139 ]; O. persica
[141 ], [142 ]
92
5-hydroxy-3′,4′,6,7-tetramethoxy flavone
O. limbata
[151 ]
93
eupatorin
O. limbata
[151 ], [152 ]
94
3′,6-dihydroxy-4′,5,7-trimethoxy-flavone
O. persica
[153 ]
95
4′,5,6,7-tetramethoxyflavone
B. cinerea
[154 ]
96
tangeretin
B. nigra
[155 ]
97
corymbosin
B. glandulosissima
[73 ], [75 ], [147 ]
98
6,5′-dihydroxy diosmetin
O. fruticosa
[156 ]
Table 7 Distribution of flavonols in Ballota and Otostegia taxa.
No
Name
Taxa
99
kaempferol
B. deserti
[157 ]; B. lanata
[29 ], [138 ], [145 ]; O. persica
[158 ]
100
quercetin
B. deserti
[157 ]; B. lanata
[29 ], [138 ], [143 ]; B. macrodonta
[159 ]; O. persica
[158 ], [160 ]
101
isorhamnetin
B. lanata
[138 ], [145 ]
102
quercetin 3,7,3′,4′-tetramethyl ether
B. undulata
[150 ]
103
5-hydroxy-3,7,4′-trimethoxyflavone
B. inaequidens
[73 ], [76 ]; B. nigra ssp. foetida
[73 ]; B. rotundifolia
[73 ]; B. saxatilis
[73 ], [75 ]
104
isokaempferide
B. hirsuta
[136 ]
105
kaempferol 3,7,4′-trimethyl ether
B. saxatils ssp. brachyodonta
[146 ]; B. undulata
[150 ]
106
kumatakenin
B. glandulosissima
[73 ], [75 ], [147 ]; B. hirsuta
[136 ]; B. nigra ssp. anatolica
[73 ]; B. nigra ssp. foetida [73 ]
107
pachypodol
B. glandulosissima
[73 ], [75 ], [147 ]; B. inaequidens
[73 ], [75 ], [147 ]; B. undulata
[150 ]
108
retusin
B. glandulosissima
[147 ]; B. inaequidens
[75 ], [76 ]; B. nigra ssp. foetida
[73 ]; B. saxatilis
[73 ]; B. saxatils ssp. brachyodonta
[146 ]
109
5-hydroxy-3,6,7,4′-tetramethoxy flavone
B. inaequidens
[73 ], [76 ]; B. saxatilis
[73 ]
110
morin
O. persica
[153 ], [160 ]
111
filindulatin
B. inaequidens
[75 ]
Table 8 Distribution of flavonoid glycosides, flavanones, and flavanols in Ballota and Otostegia taxa.
No
Name
Taxa
Flavone glycosides
112
apigenin-7-O -β -D-glucopyranoside
B. acetabulosa
[161 ]; B. deserti
[89 ], [157 ]; B. hirsuta
[136 ], [137 ]; B. lanata
[138 ], [144 ]; B. larendana
[162 ]; B. nigra ssp. foetida
[163 ]; B. pseudodictamnus
[162 ]; B. undulata
[164 ]; O. persica
[141 ], [142 ]
113
apigenin-7-O -β -D-glucuronide
B. deserti
[89 ]
114
apigenin-7-O -β - neohesperidoside
B. deserti
[89 ], [90 ]
115
acacetin-7-O -β -D-glucopyranoside
B. acetabulosa
[75 ], [134 ]
116
luteolin-7-O -β -D-glucopyranoside
B. andreuzziana
[148 ]; B. hirsuta
[136 ], [137 ]; B. lanata
[29 ], [138 ]; B. larendana
[162 ]; B. macrodonta
[159 ]; B. undulata
[150 ], [164 ]
117
luteolin-7-O -β -D-glucuronide
O. fruticosa
[156 ]
118
luteolin-7-O -β -D-rutinoside
B. hirsuta
[136 ]
119
diosmetin-7-O -β -D-glucopyranoside
B. undulata
[150 ]
120
chrysoeriol-7-O -β -D-glucopyranoside
B. acetabulosa
[75 ], [134 ], [161 ]; B. hirsuta
[137 ]; B. pseudodictamnus
[162 ]; O. fruticosa
[149 ]
121
6,4′-di-O-methyl-scutellarein-7-O -β -glucopyranoside
B. andreuzziana
[148 ]
Flavonol glycosides
122
quercetin-3-O -β -D-glucopyranoside (isoquercetin)
B. cinerea
[88 ]; B. hirsuta
[136 ]; B. lanata
[138 ]
123
quercetin-3-O -β -galactopyranoside
B. lanata
[144 ]
124
rutin
B. acetabulosa
[135 ]; B. cinerea
[88 ]; B. deserti
[157 ]; B. lanata
[29 ], [138 ], [143 ]; B. macrodonta
[159 ]; B. undulata
[164 ]
125
quercetin-3-O -α -L-rhamnopyranoside
B. lanata
[29 ], [138 ]
126
quercetin-3-O -β -L-rhamnopyranoside
B. cinerea
[165 ]
127
quercetin-7-O -β -L-rutinoside
B. andreuzziana
[148 ]
128
isorhamnetin-3-O -β -D-glucopyranoside
B. lanata
[138 ], [144 ], [145 ]; O. persica
[166 ]
129
isorhamnetin-3-O -β -D-rutinoside
B. lanata
[138 ], [144 ]
130
isorhamnetin-3-O -β -D-galactopyranoside
B. lanata
[138 ]
131
isorhamnetin-7-O -β -D- rutinoside
B. lanata
[145 ]
132
kaempferol-3-O -β -D-glucopyranoside
B. lanata
[138 ], [143 ], [145 ]
133
kaempferol-3-O -β -D-rutinoside (nicotiflorin)
B. cinerea
[88 ]; B. lanata
[138 ]
134
kaempferol-7-O -β -D-glucopyranoside
B. lanata
[138 ]
135
kaempferol-3-O -[β -D-glucopyranosyl-(1 → 2)-{β -D-glucopyranosyl-(1 → 3)}-{β -D-glucopyranosyl-(1 → 4)}-α -L-rhamnopyranoside]-7-O-[α -L-rhamnopyranoside]
O. limbata
[167 ]
Acyl flavonoid glycosides
136
apigenin-7-(p -coumaroyl)-glucoside
B. hirsuta
[136 ], [137 ]
137
apigenin-7-O -β -D-(3″-p -E -coumaroyl)glucopyranoside
B. larendana
[162 ]
138
apigenin-7-O -β -D-(4″-E -p -coumaroyl)glucopyranoside (echinaticin)
B. acetabulosa
[161 ]; O. persica
[141 ], [142 ]
139
apigenin-7-O -β -D-(6″-E -p -coumaroyl)glucopyranoside (terniflorin)
B. deserti
[89 ]; B. lanata
[138 ], [168 ], [169 ]; B. larendana
[162 ]; B. pilosa
[140 ]
140
apigenin-7-O -β -D-(2″,6″-E -dicoumaroyl)glucopyranoside
B. lanata
[168 ]
141
luteolin-7-(p -coumaroyl)glucopyranoside
B. hirsuta
[137 ]
142
luteolin-7-lactate
B. nigra
[170 ]
143
luteolin-7-O -[2-O -β -D-glucopiranosyl-lactate]
B. nigra
[170 ]
144
chrysoeriol-7-(p -coumaroyl)glucopyranoside
B. hirsuta
[137 ]
145
chrysoeriol-7-O -β -D-(3″-E -p -coumaroyl)-glucopyranoside
B. acetabulosa
[161 ]; B. pseudodictamnus
[162 ]
146
chrysoeriol-7-O -β -D-(3″-Z -p -coumaroyl)glucopyranoside
B. acetabulosa
[161 ]
147
chrysoeriol-7-O -β -D-(6″-p -coumaroyl)glucopyranoside
B. lanata
[138 ]; B. undulata
[150 ]
148
kaempferol-7-O -β -D-(6″-p -coumaroyl)glucopyranoside
B. lanata
[138 ]
149
kaempferol-3-O -β -D-(6″-p -coumaroyl)glucopyranoside
B. lanata
[138 ]
150
5,6,7,4′-tetrahydroxyflavone-7-O -β -D-(6″-E -p -coumaroyl)glucopyranoside
B. lanata
[144 ]
151
5,6,7,4′-tetrahydroxyflavone-7-O -β -D-(6″-E -caffeoyl)glucopyranoside
B. lanata
[144 ]
152
leufolins B
B. arabica
[171 ]
153
5-hydroxy-2-(4-hydroxyphenyl)-4-oxo-7-[(α -L-rhamnopyranosyl)oxy]-4H-chromen-3-yl β -D-glucopyranosyl-(1 → 2)-[β -D-glucopyranosyl-(1 → 4)]-[6-O-[(2E )-3-(4-hydroxyphenyl) prop-2-enoyl]-β -D-glucopyranosyl-(1 → 3)]-α -L-rhamnopyranoside
O. limbata
[172 ]
154
5-hydroxy-2-(4-hydroxyphenyl)-4-oxo-7-[(α -L-rhamnopyranosyl)oxy]-4H-chromen-3-yl [6-O-[(2E )-3-(4-hydroxyphenyl)prop-2-enoyl]-β -D-glucopyranosyl-(1 → 2)]-[β -D-glucopyranosyl-(1 → 4)]-[6-O-[(2E )-3-(4-hydroxyphenyl)prop-2-enoyl]- β -D-glucopyranosyl-(1 → 3)]-α -L-rhamnopyranoside
O. limbata
[172 ]
155
kaempferol-3-O -[β -D-glucopyranosyl-(1 → 4)-β -D-6″”’[4-hydroxy-(E )-cinnamoyl]glucopyranosyl-(1 → 3)-{β -D-glucopyranosyl-(1 → 2)}-α -L-rhamnopyranoside]-7-O -[α -L-rhamnopyranoside]
O. limbata
[167 ]
C-glycosyl flavonoids
156
panzeroside A
B. lanata
[173 ]
157
panzeroside B
B. lanata
[173 ]
158
isovitexin
O. persica
[158 ]
159
vicenin-2
B. aucheri
[174 ]; B. hirsuta
[136 ]; B. nigra ssp. foetida
[163 ]; O. fruticosa
[156 ]; O. persica
[166 ]
Flavanones, flavanone glycosides
160
naringenin
B. acetabulosa
[135 ]
161
naringenin-7-O -β -D-glucopyranoside
B. macrodonta
[159 ]
162
naringin
B. acetabulosa
[135 ]
163
leufolins A
B. arabica
[171 ]
Flavanols
164
epicatechin
B. acetabulosa
[135 ]; B. macrodonta
[159 ]
165
catechin
B. macrodonta
[159 ]
166
epigallocatechin gallate
B. macrodonta
[159 ]
Flavonoid glycosides, described in 24 reports, account for the vast majority of the 91 total flavonoid reports published so far, followed by flavones (23 reports). Flavonoid coumaroyl glycosides, present in 18 reports (compounds 136 –141, 143 –150, 152 –155, 163 ), have a peculiar chemotaxonomical significance and are generally considered valuable markers in the Labiatae family [137 ].
Although the majority of the flavonoids identified had already been detected in other genera of several families, some were identified for the first time.
From the AP of Ballota acetabulosa, 5 flavonoids were isolated (112, 120, 138, 145 , and 146 ). Compound 146 is a new natural flavonoid characterized as the cis isomer of chrysoeriol-7-O -β -(3″-p -coumaroyl)glucopyranoside. The trans isomer 145 (co-occurring in the same species) was previously described from other species belonging to the Lamiaceae family [161 ].
The new flavonoid coumaroyl glucosides leufolins A (163 ) and B (152 ) were isolated from the EtOAc soluble fraction of the WPs of L. urticifolia (syn. B. arabica ). Their structures were elucidated on the basis of extensive analysis of 1D and 2D NMR spectral data. Both compounds exhibited significant inhibitory potential against the enzyme butyrylcholinesterase. The unusual oxygenated pattern of the flavonoid moiety of leufolin B (153 ), devoid of hydroxyl group at C-5 [171 ], is noteworthy.
From the n -BuOH extract of the AP of Panzeria alaschanica Kuprian. (syn. B. lanata L.), 2 new flavone C-glycosides, named panzeroside A (156 ) and B (157 ), were isolated. The 2 new compounds demonstrated significant and dose-dependent analgesic and anti-inflammatory effects [173 ].
The MeOH extract of the roots of O. limbata was subjected to several chromatographic separations to give 4 new poly-glycosyl derivatives of kaempferol: compounds 135, 153, 154 , and 155 , the last 3 also carrying p -coumaroyl groups. Their rather complex structures were elucidated by extensive 1D and 2D NMR [167 ], [172 ].
Other metabolites
Apart from diterpenoids and flavonoids, several other metabolites have been identified in Ballota and Otostegia taxa: tritepenoids, steroids ([Fig. 11 ]), carboxylic acids ([Fig. 12 ]), carotenoids ([Fig. 13 ], [Table 9 ]), nitrogen containing compounds ([Fig. 13 ]), phenylpropanoids, and miscellaneous ([Fig. 14 ], [Table 10 ]).
Fig. 11 Structures of triterpenes and steroids.
Fig. 12 Structures of carboxylic acids.
Fig. 13 Structures of carotenoids and nitrogen containing compounds.
Table 9 Distribution of triterpenes, steroids, carboxylic acids, and carotenoids in Ballota and Otostegia taxa.
No
Names
Taxa
Triterpenoids
167
α -amyrin
O. fruticosa
[175 ]
168
β -amyrin
B. cinerea
[154 ], [176 ], O. persica
[177 ]
169
betulin
B. cinerea
[176 ],
170
betulonic acid
B. cinerea
[154 ]
171
friedelin
B. aucheri
[174 ], B. cinerea
[154 ]
172
lupeol
O. fruticosa
[175 ]
173
moronic acid
B. cinerea
[178 ]
174
oleaonolic acid
B. nigra
[179 ], B. cinerea
[180 ]
O. fruticosa
[156 ]
175
oleanolic acid 3-acetate
B. pilosa
[140 ]
176
ursolic acid
B. arabica
[181 ], B. nigra
[179 ], O. fruticosa
[156 ]
Steroids
177
campesterol
O. persica
[177 ]
178
3β -hydroxy-35-(cyclohexyl-5′-propan-7′-one)-33-ethyl-34-methyl-bacteriohop-16-ene
B. cinerea
[182 ]
179
leucisterol
B. arabica
[181 ]
180
β -sitosterol
B. arabica
[181 ], B. cinerea
[87 ], [154 ], [176 ], B. deserti
[24 ], B. lanata
[29 ], [143 ], B. nigra
[115 ], [183 ], B. pilosa
[140 ], O. fruticosa
[156 ], [175 ], O. persica
[177 ]
181
β -sitosterol 3-acetate
O. persica
[177 ]
182
β -sitosterol-3-O -β -D-glucopiranoside
B. cinerea
[154 ], B. deserti
[24 ], B. lanata
[29 ], [143 ], B. pilosa
[140 ]
183
stigmasterol
B. aucheri
[174 ], B. cinerea
[87 ], [176 ], [182 ], B. deserti
[24 ], B. lanata
[143 ], B. pilosa
[140 ], B. undulata
[81 ], O. integrifolia
[100 ], O. persica
[177 ]
184
stigmasterol-3-O -β -D-glucopiranoside
B. pilosa
[140 ]
Carboxylic acids
185
caffeic acid
B. acetabulosa
[135 ], B. arabica
[184 ], B. lanata
[29 ], [145 ], B. macrodonta
[159 ], B. nigra
[139 ], [185 ], O. fruticosa
[156 ], O. persica
[177 ]
186
E -caffeoyl-L-malic acid
B. hirsuta
[186 ], B. lanata
[29 ], B. nigra
[185 ], [186 ], [187 ], [188 ], [189 ], B. pseudodictamnus
[162 ], B. rupestris
[186 ]
187
4-O -caffeoylquinic acid
B. macrodonta
[159 ]
188
chlorogenic acid
B. acetabulosa
[135 ], B. lanata
[29 ], B. macrodonta
[159 ], B. nigra
[139 ], [185 ]
189
E -cinnamic acid
B. deserti
[157 ], O. persica
[158 ]
190
E -coumaric acid
B. acetabulosa
[135 ], B. hirsuta
[137 ], B. macrodonta
[159 ]
191
ellagic acid
B. macrodonta
[159 ]
192
ferulic acid
B. arabica
[184 ], B. macrodonta
[159 ], B. nigra
[139 ], O. fruticosa
[149 ]
193
fumaric acid
B. nigra
[185 ]
194
gallic acid
B. acetabulosa
[135 ], B. arabica
[184 ], B. cinerea
[176 ], B. deserti
[157 ], B. macrodonta
[159 ]
195
gentisic acid
B. macrodonta
[159 ]
196
4-hydroxy benzoic acid
B. arabica
[181 ], B. deserti
[157 ], B. macrodonta
[159 ], O. persica
[177 ]
197
jasmonic acid 5′-β -D-glucopyranosyloxy
B. cinerea
[88 ]
198
laballenic acid
B. nigra
[188 ]
199
neochlorogenic acid
B. lanata
[29 ], B. macrodonta
[159 ]
200
quinic acid
B. nigra
[185 ]
201
rosmarinic acid
B. acetabulosa
[135 ], B. macrodonta
[159 ]
202
salicylic acid
B. macrodonta
[159 ]
203
shikimic acid
B. nigra
[185 ]
204
syringic acid
B. macrodonta
[159 ]
205
tariric acid
B. cristata
[190 ]
206
urticic acid
B. arabica
[181 ]
207
vanillic acid
B. macrodonta
[159 ]
Carotenoids
208
carotene
B. lanata
[29 ]
209
lutein
B. lanata
[29 ]
210
neoxanthin
B. lanata
[29 ]
211
violaxanthin
B. lanata
[29 ]
212
zeaxanthin
B. lanata
[29 ]
Fig. 14 Structures of other compounds.
Table 10 Distribution of N -derivatives, phenylpropanoids, and other metabolites in Ballota and Otostegia taxa.
No
Names
Taxa
Nitrogen-containing compounds
213
choline
B. nigra subsp. foetida
[191 ]
214
cinerealactam E
B. cinerea
[88 ], [182 ]
215
4-hydroxyprolinebetaine
B. nigra
[188 ], B. undulata
[150 ]
216
1-methylindole-3-carboxaldehyde
B. cinerea
[87 ]
217
stachydrine
B. lanata
[138 ], [192 ], B. nigra
[188 ], B. nigra subsp. foetida
[191 ], B. undulata
[150 ]
Penylpropanoids
218
alyssonoside
B. nigra
[185 ], [187 ]
219
angoroside A
B. nigra
[187 ]
220
arenarioside
B. nigra
[94 ], [187 ], [193 ]
221
ballotetroside
B. nigra
[94 ], [185 ], [187 ], [194 ]
222
betonyoside F
B. undulata
[150 ]
223
forsythoside B
B. deserti
[89 ], [90 ], B. hirsuta
[186 ], B. nigra
[94 ], [115 ], [185 ], [186 ], [187 ], [188 ], [189 ], [193 ], B. pseudodictamnus
[162 ], B. rupestris
[186 ], B. undulata
[150 ]
224
lavandulifolioside
B. nigra
[187 ]
225
lysionotoside
B. undulata
[150 ]
226
martynoside
B. cinerea
[88 ], B. nigra
[115 ]
227
verbascoside (acteoside)
B. deserti
[89 ], [90 ], B. hirsuta
[186 ], B. lanata
[29 ], [144 ], [168 ], B. nigra
[94 ], [185 ], [186 ], [187 ], [188 ], [189 ], [193 ], [195 ], B. pseudodictamnus
[162 ], B. rupestris
[186 ], B. undulata
[150 ]
Other metabolites
228
8-O -acetylharpagide
O. fruticosa
[82 ]
229
anthroquinone1,4-dihydroxy-6,7-dimethoxy 2-methyl 3-O-β -D-glucopyranoside
B. cinerea
[165 ]
230
eutigoside A
B. acetabulosa
[161 ]
231
4-hydroxybenzaldehyde
B. macrodonta
[159 ]
232
4-methoxybenzyl benzoate
B. arabica
[184 ]
233
4-methyl-catechol
B. deserti
[157 ]
234
7-methoxy coumarin
B. lanata
[145 ]
235
oleuropein
B. acetabulosa
[135 ]
236
phytol
B. deserti
[24 ], B. nigra
[183 ], B. nigra subsp. anatolica
[196 ]
237
stachyose
B. nigra subsp. foetida
[197 ]
238
undatuside A
B. cinerea
[88 ]
239
verminoside
B. undulata
[150 ]
The first study on Ballota and Otostegia genera was carried out in 1911 by Piault [197 ], which isolated the tetrasaccharide stachyose from the roots of B. nigra subsp. foetida (237 ). The following report dates 1934 when, from the same species, Balansard isolated choline (213 ) and stachydrine (217 ) [191 ].
Phenylpropanoids (218 –227 ) occur within 10 compounds and, apart from forsythoside B (223 ) and verbascoside (227 ), are present in several species. The main source of this class of compounds is B. nigra from which ballotetroside (221 ), a new derivative, was isolated [194 ].
Triterpenoids (10 compounds) and steroids (8 compounds) are represented by rather common metabolites, although there are some exceptions. For example, moronic acid (173 ) was isolated for the first time in B. cinerea
[178 ], and the new steroid leucisterol (179 ), as well as the new peroxy acid urticic acid (206 ), were isolated from the chloroform soluble fraction of the WP of B. arabica (syn. L. urticifolia ). Leucisterol (179 ) showed potent inhibitory activity against butyrylcholinesterase enzyme [181 ]. Recently, the new, structurally quite complex, bacteriohopane-type derivative 178 was isolated from B. cinerea (syn. Roylea cinerea (D. Don) Baill.) [182 ]. In the same work, the β -lactam cinerealactam E (214 ) [88 ] was also detected. Both compounds were shown to have a significant effect on the decline in blood glucose levels supporting the role of B. cinerea in Ayurvedic medicine for diabetes.
In [Table 11 ], the occurrence of all of the metabolites in the single taxa is summarized. For some common compounds, whose structures have not been depicted in this review, the trivial name is reported.
Table 11 Occurrence of non-volatile metabolites in taxa of Ballota and Otostegia .
Taxa
Diterpenes
Flavonoids
Others
B. acetabulosa
5, 18, 25, 33
76, 80, 82, 85, 112, 115, 120, 124, 138, 145, 146, 160, 162, 164
185, 188, 190, 194, 201, 230, 235
B. africana
25
B. andreuzziana
25
83, 86, 116, 121, 127
B. antalyensis
5, 18
B. arabica
75
152, 163
176, 179, 180, 182, 185, 192, 194, 196, 206, 232
B. aucheri
4, 5, 10, 27, 28, 29, 30, 31, 34, 44, 46
159
185, 188, 190, 194, 201, 230, 235
B. cinerea
12, 13, 14, 15, 16, 45, 74
87, 95, 122, 124, 126, 133
168 –171, 173, 174, 178, 180, 182, 183, 194, 197, 214, 216, 226, 229, 238 ; cetyl alcohol, glucose, fructose, arabinose, palmitic acid, stearic acid, oleic acid, oxalic acid, tartaric acid [176 ]; hentriacontane, triacontane [116 ]; pentacosane, octacosanol [154 ]
B. cristata
5, 18, 25
204 ; linoleic acid, oleic acid, palmitic acid, stearic acid, linolenic acid [190 ]
B. deserti
2, 3, 19, 17, 20, 21, 24, 37 –40
76, 99, 100, 112 –114, 124, 139
180, 182, 183, 189, 194, 196, 223, 227, 233, 236
B. glandulosissima
81, 88, 90, 97, 106, 107, 108
B. hirsuta
25
76, 78, 80, 81, 85, 89, 104, 106, 112, 116, 118, 120, 122, 136, 141, 144, 159
186, 190, 223, 227
B. hispanica
51, 52
B. inaequidens
5, 25
79, 80, 88, 103, 107 –109, 111
B. lanata
5, 32
76, 79, 85, 88, 91, 99, 100, 101, 112, 116, 122 –125, 128 –132, 134, 139, 140, 147 –151, 156, 157
176, 180, 182, 183, 185, 186, 188, 199, 208 –212, 217, 227, 234
B. larendana
5, 18
112, 116, 137, 139
B. latibracteolata
18
80
B macrodonta
18
100, 116, 124, 161, 164 –166
185, 187, 188, 190 –192, 194 –196, 199, 201, 202, 204, 207, 231
B. nigra
1, 5, 32,73
76, 80, 85, 91, 96, 142, 143
174, 176, 180, 182, 185, 186, 188, 192, 193, 198, 200, 203, 215, 217 –221, 223, 224, 226, 227, 236 ; oxalic acid, aconitic acid, citric acid, ascorbic acid, malic acid [185 ]; linoleic acid α-linolenic acid, oleic acid, palmitic acid, stearic acid [198 ]
B. nigra subsp. anatolica
106
236 ; 10-undecenoic acid, myristic acid, palmitoleic acid, palmitic acid, 11,13-dimethyl-12-tetradecen-1-ol acetate, linoleic acid, oleic acid, linolenic acid, stearic acid, arachidic acid, 7-methyl-6-hexadecenoic acid, behenic acid [196 ]
B. nigra subsp. foetida
5, 9, 10, 37, 47
103, 106, 108, 112, 159
213, 217, 237 ; palmitic acid, stearic acid, octadecenoic acid, octadecadienoic acid, octadecatrienoic acid [199 ]
B. nigra f. uncinata .
18
B. pilosa
76, 139
175, 180, 182 –184
B. pseudodictamnus
5, 33, 36
79, 112, 120, 145
186, 223, 227
B. pseudodictamnus subsp. lycia
18, 25
B. rotundifolia
18, 25
79, 80, 103
B. rupestris
5, 8, 49
186, 223, 227
B. saxatilis
5, 18, 25, 33
80, 103, 108, 109
B. saxatilis subsp. brachyodonta
5, 18
80, 105, 108
B. undulata
5, 8, 10, 26
90, 102, 105, 107, 112, 116, 119, 124, 147
183, 215, 217, 222, 223, 225, 227, 239 ; (−)-carvone, [81 ]
O. fruticosa
5, 22, 23, 34, 35, 41, 42, 46, 50
84, 98, 117, 120, 159
167, 174, 176, 180, 185, 192 ; octacosane, palmitic acid, linoleic acid, arachidic acid [175 ]
O. integrifolia
43, 48
183 ; pentatriacontane [100 ]
O. limbata
6, 7, 53 –72
92, 93, 135, 153 –155
O. persica
56, 59, 71, 72
77, 91, 94, 99, 100, 110, 112, 128, 158, 159
168, 177, 180, 181, 183, 185, 189, 196 ; gerianol, eugenol, ceryl alcohol, hentiacontane [177 ]
EOs
The chemical composition of EOs obtained from 21 species among Ballota and Otostegia taxa has been investigated. They are mainly distributed in the Mediterranean area, whereas the Otostegia species are almost totally distributed in western Asia and Ballota lanata , syn. Panzeria (Panzerina ) lanata , found in eastern Asia (Siberia and Mongolia). The major compounds (> 3%) occurring in the chemical composition of the EOs are reported in [Table 12 ].
Table 12 Main compounds (> 3%) of the essential oils from Ballota and Otostegia taxa.
Taxa
Origin
Main compounds
Ref.
B. andreuzziana
F
G. Akhdar, Libya
caryophyllene (63.1), cis-γ -bisabolene (26.3), selinene (5.0)
[200 ]
B. aucheri
AP
Fars, Iran
α -cadinol (21.0), dehydroaromadendrene (11.8), β -caryophyllene (8.1), carvone (6.4), spathulenol (6.0), linalool (4.8), (Z )-methyl isoeugenol (4.1), α -santalene (3.5)
[201 ]
B. deserti
AP
Djelfa, Algeria
germacrene D (45.7), β -bourbonene(4.0), α -terpinolene (3.9), δ -cadinene (3.8), 1-octen-3-ol (3.7), α -copaene (3.5)
[202 ]
AP
Ghardaïa, Algeria
9-methyl-undecene (21.3), δ -cadinene (12.2), germacrene D (11.9), cis -phytol (7.7), α -cubebene (4.4)
[203 ]
AP
Algerian Sahara
tetracosane (31.1), germacrene D (7.9), δ -cadinene (6.5), α -cadinol (6.3) t -cadinol (5.8), β -elemene (3.8)
[204 ]
B. hispanica
AP
Sicily, Italy
α -elemol (10.9), α -ylangene (8.5), γ -dodecalactone (5.1), manoyl oxide (4.8), γ -eudesmol (4.2), β -eudesmol (3.7), 1-pentadecene (3.7), germacrene D (3.5),
[205 ]
B. lanata
AP
Buryatia, Russia
palmitic acid (14.3), camphor (12.4), α -pinene (10.3), linalool (9.1), β -caryophyllene (8.3), terpinen-4-ol (6.4), phytol (4.8), caryophyllene oxide (4.2), p -mentha-3-en-8-ol (3.3)
[29 ]
AP
Gobi, Mongolia
camphor (14.4), α -pinene, (11.3), terpinenol-4 (5.3), 6,10,14-trimethyl-2-pentadecanone (4.7), β -caryophyllene (3.5), β -humulene (3.2), α -thujene (3.1)
[206 ]
B. macedonica
AP
Debar, Macedonia
germacrene D (24.6), (E )-caryophyllene (16.5), carotol (13.7), caryophyllene oxide (3.5)
[207 ]
AP
Prizren, Serbia
carotol (52.1), germacrene D (8.6), (Z )-hex-3-en-1-ol (7.0), (E )-caryophyllene (6.5) oct-1-en-3-ol (3.8)
[207 ]
B. nigra
AP
Mazandaran, Iran
caryophyllene oxide (7.9), epi -α -muurolol (6.6), δ -cadinene (6.5), α -cadinol (6.3), γ -amorphene (4.3), β -bourbonene (4.1), 6,10,14-trimethyl-2-pentadecanone (4.0), (E )-caryophyllene (4.0), germacrene D (3.8), aromadendrene (3.4), γ -muurolene (3.2), germacrene D-4-ol (3.2), α -bisabolol (3.2), α -amorphene (3.0)
[208 ]
S
Jadovnik Mt., Serbia
β -caryophyllene (35.4), germacrene D (27.4), α -humulene (7.4), δ -cadinene (3.8), (E )-phytol (2.5)
[209 ]
L
Jadovnik Mt., Serbia
β -caryophyllene (39.1), germacrene D (35.7), α -humulene (10.4), (E )-phytol (3.8)
[209 ]
R
Jadovnik Mt., Serbia
p -vinylguiacol (9.2), borneol (7.5), myrtenol (7.1), trans -pinocarveol (5.2), 1-octen-3-ol (5.1), pinocarvone (4.4), 2-methyl-3-phenylpropanal (4.3), p -cymen-8-ol (4.3), trans -carveol (3.5)
[209 ]
AP
Golestan, Iran
β -pinene (39.0), α -pinene (34.5), sabinene (7.7), α -phellandrene (4.1)
[210 ]
corollas
Kharkov, Ukraine
palmitic acid (573)a , 2,2,6-trimethyl-4-methylene-2H-pyran (172)a , hexahydrofarnesylacetone (167)a , miristic acid (100)a , caryophyllene oxide (57)a , pentadecanoic acid (50)a , palmitoliec acid (40)a , germacrene D (40)a a mg/kg
[211 ]
calyx
Kharkov, Ukraine
palmitic acid (1620)a , dodecanal (519)a , palmitoliec acid (306)a , miristic acid (271)a , pentadecanoic acid (182)a , lauric acid (67)a , trans -isoelemicin (67)a , hexahydrofarnesylacetone (60)a , pentadecene (54)a , methyleugenol (40)a a mg/kg
[211 ]
L
Kharkov, Ukraine
palmitic acid (656)a , palmitoliec acid (197)a , miristic acid (187)a , pentadecanoic acid (121)a , farnesylacetone (69)a , dihydroactinidiolide (44)a a mg/kg
[211 ]
S
Kharkov, Ukraine
methylsalicilate (313)a , palmitic acid (130)a , 2,2,6-trimethyl-4-methylene-2H-pyran (42)a , miristic acid (42)a a mg/kg
[211 ]
B. nigra L. subsp. anatolica
AP
Mazandaran, Iran
germacrene D (18.1), nerolidol epoxyacetate (15.4), sclareol oxide (12.1), linalyl acetate (11.5), β -caryophyllene (10.5), spathulenol (9.0), linalool (5.2), longipinene epoxide (4.7)
[212 ]
F
Çamlica, Turkey
hexenal (21.2), (E )-β -caryophyllene (10.0), germacrene D (7.8), cis -3-hexene-1-ol (6.8), pentanal (6.9), limonene (5.2), (E )-2-hexenal (3.0)
[213 ]
AP
Muğla, Turkey
hexadecanoic acid (40.9), β -bisabolene (13.4), hexahydrofarnesyl acetone (7.9), 1-isobutyl-4-isopropyl-2,2-diemethyl succinate (6.6), β -eudesmol (3.5)
[214 ]
AP
Western Turkey
1-hexacosanol (26.7), caryophyllene oxide (9.3), germacrene-D (9.3), α -selinene (8.7), Z-8-octadecen-1-ol acetate (7.1), 2,5-di-tertoctyl-p-benzoquinone (7.3), arachidic acid (6.0), tetracosane (4.5), heneicosane (4.4), heptacosane (4.3), 2-methyl-1-hexadecanol (3.3), octadecane (3.0), butyl phthalate (3.0)
[196 ]
B. nigra subsp. foetida
AP
Pisa, Italy
β -caryophyllene (25.1), germacrene D (24.2), 1-octen-3-ol (7.3), (E )-2-hexenal (6.1), α -humulene (4.3), caryophyllene oxide (4.2)
[215 ]
AP
Urbino, Italy
β -caryophyllene (20.0), germacrene D (18.0), caryophyllene oxide (15.0), 1-octen-3-ol (6.8), (E )-2-hexenal (6.1), α -humulene (4.5), β -bourbonene (3.2)
[216 ]
AP flowering
Urbino, Italy
β -caryophyllene (22.6), caryophyllene oxide (18.0), germacrene D (16.5), (E )-2-hexenal (6.5), 1-octen-3-ol (5.5)
[217 ]
AP fruiting
Urbino, Italy
β -caryophyllene (21.8), caryophyllene oxide (20.5), germacrene D (13.1), (E )-2-hexenal (11.2), β -pinene (4.4), limonene (4.1), 1-octen-3-ol (3.5), linalool (3.5)
[217 ]
AP
Nis, Serbia
(E )-phytol (56.9), germacrene D (10.0), β -caryophyllene (4.7), caryophyllene oxide (3.6), (E)-β -ionone (3.4)
[207 ]
AP
Brac, Croatia
germacrene D (23.1), β -caryophyllene (20.3), caryophyllene oxide (6.2), caryophylladienol I (3.3), (E )-2-hexenal (3.1), hexadecanoic acid (3.1), α -humulene (3.0)
[218 ]
B. nigra subsp. kurdica
AP
Kurdistan, Iran
caryophyllene oxide (39.4), β -caryophyllene (24.9), germacrene D (7.6), 1-undecene (4.2), isoaromadendrene epoxide (3.2)
[219 ]
B. nigra f. uncinata
AP
Konya, Turkey
caryophyllene oxide (21.2), hexadecanoic acid (19.9), β -caryophyllene (18.9), germacrene D (4.6), hexahydrofarnesyl acetone (4.4), spathulenol (4.2), caryolphyllenol II (3.8); bicyclogermacrene (3.7)
[214 ]
B. pseudodictamnus
AP
Crete, Greece
caryophyllene oxide (22.4), phytol (11.9), γ -muurolene (11.4), (E )-caryophyllene (10.7), α -copaene (6.1), β -cucubene (5.3), hexahydrofarnesyl acetone (3.5)
[220 ]
B. saxatilis
AP
Amman, Jordan
linalool (14.6), caryophyllene oxide (11.0), acorenone (9.3), β -caryophyllene (7.9), germacrene D (7.6), 1-octen-3-ol (3.6), β -bourbonene (3.0)
[215 ]
AP
Kfardin, Lebanon
linalool (11.2), (E )-β -caryophyllene (8.8), caryophyllene oxide (6.3), (E )-2-hexenal (5.6), hexadecanoic acid (4.9), (Z,Z )-9,12-octadecadienoic acid (3.4)
[218 ]
B. saxatilis subsp. brachyodonta
AP
Mersin, Turkey
(E )-β -caryophyllene (23.9), epi-bicyclosesqui-phellandrene (20.2), caryophyllene oxide (10.5), γ -elemene (5.5), thymol (4.1)
[221 ]
Ballota schimperi
L
Yemen
τ -cadinol (9.3), β -caryophyllene (8.8), bornyl formate (5.2), myrtenyl formate (3.8), spathulenol (3.2), β -selinene (3.0)
[222 ]
B. sechmenii
Turkey
linalool (5) (ratio (+)-linalool: (−)-linalool = 27 : 73)
[223 ]
B. undulata
AP
Naur, Jordan
germacrene D (19.1), bicyclogermacrene (11.6), viridiflorol (6.0), 1-octen-3-ol (3.5), epi -10-γ –eudesmol (3.1)
[215 ]
AP
Kfardin, Lebanon
germacrene D (16.0), bicyclogermacrene (10.4), 9,12-octadecadienoic acid (5.3), hexadecanoic acid (4.5), dihydroactinidiolide (3.4)
[218 ]
O. fruticosa
AP cultivated
El-Mansoura, Egypt
thymol (43.7), γ -terpinene (16.4), p -cymene (12.4), (E )-β -caryophyllene (9.5)
[224 ]
AP
Sinai, Egypt
caryophyllene oxide (60.8), β -bisabolene (9.2), 4-decyne (5.1), α -cis -bergamotene (4.4), β -bourbonene (4.2), linalyl acetate (4.2)
[175 ]
O. integrifolia
Ls
North Shoa, Ethiopia
α -pinene (31.3), 1-octen-3-ol (11.8), β -caryophyllene (11.3), linalool (6.6), cis -β -ocimene (5.9), germacrene D (3.3)
[56 ]
O. michauxii
AP
Zagros, Iran
caryophyllene oxide (20.1), trans -verbenol (10.2), linalool (5.3) and humulene epoxide II (4.6)
[225 ]
AP
Fars, Iran
dillapiole (23.9), 2-methylbenzofuran (12.9), α -pinene (8.1), δ -cadinene (6.1), 1-octen-3-ol (4.9), caryophyllene oxide (4.8), linalool (4.5), (E )-β -caryophyllene (3.6)
[226 ]
O. persica
AP
Fars, Iran
dillapiole (43.1), trans -verbenol (9.6), hexadecanoic acid (5.7), isospathulenol (4.5)
[227 ]
L
Sistan, Iran
hexahydrofarnesyl acetone (14.3), trans -verbenol (10.2), geranyl acetone (6.5), pentadecane (5.9), hexadecane (5.9), α -pinene (4.5), trans -anethole (4.5), verbenone (3.5), 1-octen-3-ol (3.0)
[228 ]
F
Sistan, Iran
α -pinene (13.6), trans -verbenol (9.2), linalool (6.8), hexadecane (5.5), caryophyllene oxide (4.8), pentadecane (4.6), trans -carveol (4.0), 1-octen-3-ol (3.8), geranyl acetone (3.7), heptadecane (3.3)
[228 ]
flowering AP
Kerman. Iran
hexadecanoic acid (31.7), pentacosane (29.5), α-copaene-8-ol (5.9), hexadecanoic acid methylester (4.8), caryophyllene oxide (3.8), trans -damascenone (3.7)
[228 ]
F
Kerman. Iran
α -pinene (17.2), 1-octen-3ol (13.4), cubenol (7.3)
[229 ]
fruits
Kerman. Iran
diisooctyl phthalate (45.0), hexadecanoic acid (11.1)
[229 ]
All papers are quite recent, they have been published starting in 2002, except for one [224 ], published in 1995.
The first published paper concerns the analysis of an Egyptian O. fruticosa
[224 ] species, cultivated in the station of faculty of agriculture of Mansoura University, containing mainly monoterpenes with a high level of thymol (43.7%). A recent re-investigation of the same species collected wild in the Sinai region showed a composition strongly dominated by sesquiterpenes with the caryophyllene oxide being the most abundant component (60.8%) [175 ].
For the single species Ballota sechmenii Gemici & Leblebici, only the relative content of linalool (5.0%) and its enantiomeric composition, (+)-isomer (26.9%), (−)-isomer (73.1%) have been determined [223 ]. No other component of the EO was reported.
With only a few exceptions, such as B. lanata
[29 ], [206 ] and 2 B. nigra specimens collected in the Golestan region of Iran [210 ] and Ukraine [211 ], respectively, Ballota species EOs are mainly composed of sesquiterpenes with caryophyllene, caryophyllene oxide, and germacrene D often identified as main compounds. On the contrary, in B. lanata, monoterpenes are the dominant components, similar to the B. nigra of Golestan. The analysis of EOs of different plant parts of Ukrainian B. nigra , showed that fatty acids are the most relevant compounds with sesquiterpenes occurring only in the corallas. However, a nonconventional method of EO extraction was applied.
The Otostegia species studied to date shows controversial results. The case of the O. fruticosa , discussed above, and of the O. michauxii , collected in 2 different locations, are emblematic. In fact, the O. michauxii from southern Zagros of Iran show caryophyllene oxide as a main compound [225 ], whereas the one collected in the Fars province of Iran had an equal amount of monoterpenes and sesquiterpenes [226 ]. In O. integrifolia , the monoterpene α -pinene occurs in 31% of the oil [56 ], whereas in some collections of O. persica , in several places of southeast Iran, a clear trend cannot be observed.
Biological Properties
This section deals with the corpus of scientific evidence related to the claims of the biological effects of Ballota and Otostegia genus utilized in traditional medicine ([Table 2 ]). The most widespread usage of the plants is as an aqueous infusion of the drug, which is normally made from the WP dried. The beneficial effects should normally be associated with the presence of polar or water-soluble active principles. Indeed, most of the cited literature deals with the effect of the extract obtained from leaves, stems, roots, flowers, WPs in polar solvent, such as EtOH, MeOH and water, as well as mixture of them. However, investigations concerning the bioactivity of fractions obtained with less polar solvents, such as choloroform, n -Hex, and EtOAc are also present. In some cases, isolated individual compounds were assessed for their bioactivity. In other cases, the EOs are the focus of the research and their composition is investigated and correlated to the bioactivity observed for that species.
In this report, a selection of the more relevant results, obtained with rigorous and well-defined methodological approaches, are taken into consideration. Redundant investigations that report data concerning the same combinations of plant species and biological targets can often be found in literature, in particular concerning antimicrobial activity.
Antioxidant activity
Many of the effects of Ballota and Otostegia reported in [Table 2 ] may be related by their general antioxidant activity, which is well documented in the literature. This activity is generally attributed to the presence of phenolic compounds that are ubiquitous in these genera. In a few cases, terpenoidic compounds, in particular diterpenes, were also identified as the source of antioxidative properties of the drug. The mechanisms of action may include oxygenated radicals scavenging, inhibition of the enzymatic peroxidation, etc. Furthermore, the variety of antioxidant activity evaluation protocols utilized often make it difficult to directly compare the effects of different species. Nevertheless, the antioxidant activity of many Ballota and Otostegia plants has been interestingly and undoubtedly demonstrated. These findings are summarized in [Table 13 ], where for each investigation reviewed the species involved is reported together with its provenience, the extraction mode, and the testing procedure implemented.
Table 13 The antioxidant activity of Ballota and Otostegia taxa.
Species
Origin
Sample preparation (plant part-solvent)
Test
Ref.
B. acetabulosa, B. antalyanse, B. cristata, B. glandulosissima, B. inaequidens, B. larendana, B. latibracteolata, B. macrodonta, B. nigra ssp. anatolica, B. nigra ssp. foetida, B. nigra, B. nigra ssp. uncinata, B. pseudodictamnus ssp. lycia, B. rotundifolia, B. saxatilis ssp. brachyodonta, B. saxatilis
Turkey
L: EtOAc, MeOH, W
FRAP (% inhib.): B. antalyense (1.34) MeOH extr., B. saxatilis ssp. brachyodonta MeOH extr. (1.28) B. saxatilis MeOH extr. (1.12); B. antalyense W extr. (1.24)
[230 ]
B. antalyense, B. macrodonta, B. glandulosissima, B. larendana, B. pseudodictamnus, B. nigra ssp. anatolica, B. rotundifolia, B. saxatilis ssp. brachyodonta, B. saxatilis
Turkey
L: EtOH/W 3 : 1
Superoxide anion formation quenching (SAFQ): IC50 0.50 to 0.87 mg/mL; Liver rats lipid peroxidation (LO): not significant
[231 ]
B. antalyense, B. macrodonta, B. glandulosissima
Turkey
L: EtOH/W 3 : 1
SAFQ: 0.50, 0.51, 0.51
[231 ]
B. inaequidens, B. glandulosissima, B. saxatilis, B. macrodonta, B. antalyense
Turkey
L: EtOH/W 3 : 1
LO (mg/mL): 12 to 20 mg/mL
[231 ]
B. inaequidens, B. glandulosissima
Turkey
L: EtOH/W 3 : 1
LO: (mg/mL) 12 and 15
[231 ]
B. aucheri
Pakistan
AP: 70% MeOH
DPPH: IC50 (µg/mL) 2.23
[232 ]
B. cinerea
India
AP: EtOH, then partition in CHCl3 , EtOAc, n -BuOH, W
DPPH: IC50 (µg/mL) 85 – 661; FRAP (mmolFe/g) 0.14 – 0.59; ABTS IC50 (µg/mL) 60 – 840; ORAC (TEAC mM) 8.55 – 36.0
[233 ]
B. deserti
Tunisia
L: MeOH, then partition in pet. ether, CHCl3 , EtOAc, BuOH
DPPH: IC50 (mg/mL) 0.85 – 2.50; ABTS: IC50 (mg/mL) 0.35 – 13, pet. Ether inactive
[25 ]
B. deserti
Algeria
EO
DPPH: IC50 µg/mL 35.9
[204 ]
B. deserti
Algeria
AP: CH2 Cl2 , MeOH, then isolat. compds: 114, 223, 227
ABTS: IC50 (g/mmol) 0.14 – 3.50
[90 ]
B. deserti
Algeria
AP: CH2 Cl2 , MeOH, then isolat. compds: 76, 112 –114, 139, 223, 227
ABTS: EC50 (mol %) 0.09-> 0.50; CUPRAC: E‰ (L/mol/cm) 0.01 – 0.80; DPPH: EC50 (mol %) 0.39 – > 1.5, 9 and 12 oxidant or pro-oxidant
[89 ]
B. hirsuta
Algeria
L: W/MeOH 1 : 1, then partition in EtOAc, CHCl3 , n -BuOH
DPPH IC50 (mg/mL): 0.35 extr., 0.07 EtOAc, 0.26 CHCl3 , 0.12 n -BuOH
[234 ]
B. nigra
ex vitro
Shoots: MeOH
DPPH EC50 (mg/mL): 56.0 – 202.6, FRAP (µmol/g) 331.5 – 642.4; LPO (% inhb.) 20.97 – 36.05
[235 ]
B. nigra
Czech Republic
L: W
DPPH IC25 (µg/mL) 4.81; X/XO (µg/mL) 14.6; HClO scav. 80% at 500 µg/mL; NO scav. IC25 (µg/mL) 122
[185 ]
B. nigra
France
L: 50% EtOH, then isolat. compds: 186, 220, 221, 223, 227
O2
2- scav: IC50 (µg/mL) 30.6 – 149.0; H2 O2 scav.: IC50 (µg/mL) 2.3 – 11.2; HClO scav.: IC50 (µg/mL) 1.5 – 9.3; OH rad. scav.: IC50 (µg/mL) 26.7 – 64.7.
[236 ]
LDL-Ox: ED50 (µM) 1.0 – 9.5
[237 ]
B. nigra ssp. anatolica
Turkey
WP: PE, Ac, MeOH, W
ABTS (% inhib. at 100 mg/mL): 72 – 80
[196 ]
B. rotundifolia
Turkey
AP: MeOH
DPPH (µg/mg) 138.0; LPO (% inhib.): 35.97
[238 ]
B. undulata
Jordan
L: MeOH: compds: 116, 119, 147, 222, 223, 225, 227, 239
ABTS, TEAC (mM) 0.68 – 1.67
[150 ]
B. pseudodictamnus, B. acetabulosa
Grecia
L: MeOH
Activity equal to α-tocopherol in Umezawa essay
[239 ]
O. integrifolia
Ethiopia
L: EtOAc, MeOH
DPPH: 82.9 MeOH, 32.7 EtOAc;
[240 ]
O. integrifolia
Ethiopia
EO
DPPH: EC50 (µL/mL) 5.32
[56 ]
O. limbata
Pakistan
WP: MeOH
DPPH: IC50 (mg/mL) 13.53 – 129.52; FRAP, (mM/mL) 88.86 – 334.27; LPO: ([% inhib.] 12.67 – 61.76).
[241 ]
O. limbata
Pakistan
AP: MeOH then solubilization in n -Hex, CHCl3 , EtOAc, BuOH, MeOH, W
DPPH: EC50 (µg/mL) 60 – 350; FRAP (mmol/mg) 5 – 41; ABTS, TEAC (µmol/g) 30 – 139
[242 ]
O. persica
Iran
WP: MeOH, then solubilization in n -Hex and CHCl3
LPO (% inhib.) 95.87 MeOH, 2.5 nHex., 1.9 CHCl3
[158 ]
O. persica
Iran
EO
DPPH: IC50 (mM) 9.76
[228 ]
O. persica
Iran
EO in flowering EO in fruiting
DPPH: IC50 (µg/mL) 19.8, 29.2; LPO (% inhib.) 93.8, 63.0
[229 ]
O. persica
Iran
AP: MeOH
LPO (% inhib.) 95.87
[160 ]
O. persica
Iran
AP: MeOH, EtOAc
DPPH: IC50 0.49 mg/mL for both
[141 ]
O. persica
Iran
L: 70% EtOH, then partition in PE, EtOAc, CHCl3 , n -BuOH, MeOH
DPPH: IC50 (µg/mL) 170 (EtOH) to 1580 (pet. Et.)
[243 ]
As it can be inferred from the analysis of [Table 13 ], B. nigra and O. persica are the most studied species. Their antioxidant activity was evaluated in the EO [196 ], [228 ], [229 ], as well as in different polarity fractions (extraction solvents: PE, Ac, EtOAc, MeOH, EtOH, water) of the extract obtained from leaves, AP, and the WP. In some cases, a number of individual active compounds were isolated and identified. The following were purified from leaf extract (water/EtOH 1 : 1) of B. nigra collected in France [236 ], [237 ]: (+)-(E )-caffeoyl-L-malic acid (186 ), verbascoside (227 ), forsythoside B (223 ), arenarioside (220 ), and ballotetroside (221 ). On the other hand, morin (110 ) and quercetin (100 ) were isolated from the bio-guided purification of the methanolic extract of O. persica
[160 ]. In an interesting study [236 ], some chemical indicators of the intracellular inflammatory cascade reaction of the neutrophils were evaluated: superoxide anion, H2 O2 , HClO, and OH radical. All of the compounds were found to be active, inhibiting the development of oxygenated species to various degrees; only ballotertoside (221 ) was inactive in the superoxide anion and OH radical tests. In another study [237 ], the same authors evaluated all 5 molecules for their inhibitory efficacy with respect to the oxidation of low-density lipoproteins (LDL). They were also assessed for their Cu(II) chelating power, as this is the well-known mechanism of action of the antioxidant quercetin. None of them were able to behave as a copper chelating agent. The infusion of B. nigra leaves from the Czech Republic was shown to possess outstanding antioxidant activity, in particular by DPPH, NO, and superoxide anion scavenging ability [185 ]. On the contrary, no OH radical scavenging ability was revealed. In terms of organic acids and polyphenol content of the infusion, the composition was determined by HPLC/DAD and HPLC/UV analysis and the authors inferred the correlation between these compounds and the observed antioxidant activity. The crude methanolic extract of AP of B. hirsuta
[234 ] and O. limbata
[241 ] were further partitioned in several solvents in order to select phenolic enriched subfractions following the solubility properties of different compounds, the EtOAc fraction being the most active in both cases: in the DPPH test, the IC50 were 13.53 µg/mL for O. limbata and 70.0 µg/mL for B. hirsuta . On the contrary, the less active fraction of the extract was the one in H2 O for B. nigra (129.5 µg/mL) and the one obtained in CHCl3 for B. hirsuta (260 µg/mL). A significant difference in the antioxidant power of different solvent fractions was found for O. persica collected in Iran [158 ]. The fraction soluble in MeOH showed LPO inhibition in the NH4 SCN test comparable to that of α -tocopherol (95.87%). On the other hand, the fractions soluble in n -Hex and CHCl3 were poorly active (2.5 and 1.9%, respectively). These variations in the antioxidant activity, evidenced by different partition solvents, can be related to the affinity of the active metabolites (poly-phenols and flavonoids) to these solvents.
The relation between the antioxidant power of O. persica extract (AP, 70% MeOH) and the protective effect against the damages caused by the oxidative stress on the endothelium cells, was investigated in vitro on a human cell line: the umbilical vein endothelial cells [244 ]. No toxicity was revealed up to 250 µg/mL of extract. The oxidative effects were induced by H2 O2 and evaluated by the cell viability essay (MTT), intracellular and extracellular total peroxides test, and FRAP. The extract significantly reduced the effect of H2 O2 in a dose-dependent fashion (50 – 250 µg/mL).
The direct correlation between the phenolic compounds content is rather general, although some exceptions are also known. For example, in an investigation of the antioxidant properties of O. limbata from Pakistan [242 ], the methanolic extract was subsequently divided in fractions soluble in n -Hex, CHCl3 , EtOAc, n -BuOH, MeOH, and H2 O, and total content in phenolic compounds and flavonoids was determined. The EtOAc fraction resulted the one with the highest antioxidant power (EC50 TPPH test 60.9 µg, total phenolic compounds 1119 mg), even if the higher phenols content was found in n -Hex (3908 mg, EC50 226.1 µg) and 1-butanol (3037 mg, EC50 96.3 µg).
The antioxidant activity of apigenin-7-O-(6″-O -[E ]-coumaroyl)-β -glucopyranoside (139 ), a flavonoid glycoside isolated from the B. lanata (syn. P. alaschanica ) AP collected in China [169 ], was determined in vivo by evaluating its lipid peroxidation inhibitory activity. A diabetes mellitus–related oxidative stress was induced in rats with a 7 week treatment of STZ. Afterward, the plasma concentration of malondialdehyde (MDA), co-enzyme Q9 , α - and γ -tocopherol were measured. A normalization in MDA values was observed in animals treated with 60 mg/kg b. w. of compound 139 , while the Q9 and γ -tocopherol level remained comparable with those of negative controls.
Anti-inflammatory activity
This is mainly attributed to the presence of flavonoids and tannins in both genera. The bioactivity is generally evaluated in in vivo models. The aqueous extract of Ballota glandulosissima Hub.-Mor. & Patzak leaves collected in Turkey [245 ] administrated to rats with carrageenan-induced paw edema (100 mg/kg b. w.) was able to induce a significant reduction of the edema volume (32%). The aerial part aqueous extract of Ballota inaequidens Hub.-Mor. & Patzak from Turkey [246 ] showed similar effects by reducing paw edema in rats in a dose-dependent manner: the volume reduction coefficient ranged from 58 to 86% by administrating 50 to 200 mg/kg b. w. per day of extract. This species also showed significant, positive dose-dependent results in the abdominal stretching test in mice: 44 – 91% reduction by administrating 30 – 100 mg/kg b. w. per day of dry extract. A relevant anti-inflammatory activity was also demonstrated in the methanolic extract of the AP of B. pseudodictamnus . This species is included in a comparative study on the anti-inflammatory activity of endemic flora from Libya [247 ]. In the mice paw edema test, a single dose of 500 mg/kg b. w. reduced the edema volume by 51%.
The same animal model was employed in the evaluation of the anti-inflammatory activity of the crude extract of the aerial part of O. persica
[166 ], as well as the fractions soluble in organic solvents of different polarities (PE, CHCl3 , EtOAc, n -BuOH, MeOH). The analgesic activities were also investigated. Both the fraction in BuOH and in MeOH were able to reduce the edema volume, the last one showing an efficacy comparable to that of indomethacin. Furthermore, the MeOH fraction showed analgesic activity with EC50 of 85.9 mg/kg b. w. Two active compounds were isolated from the methanolic fraction: vicenin-2 (159 ) and isorhamnetin-3-O -β -D-glucopyranoside (128 ).
The extract of B. deserti (Algeria) was obtained from AP with PE, dichloromethane, and MeOH. Their anti-inflammatory activity was assessed, once again with the paw edema model, using Wister albino rats [248 ]. The edema volume was reduced by 85.5% by a dose of 200 mg/kg b.w after 3 h of the MeOH extract, while acute toxicity signs were absent until 5000 mg/kg b. w. The lowest, but still significant effect, was obtained in another study [248 ] with the aqueous extract of the same taxa: edema volume was reduced after 3 h from 11 to 44% at variable doses from 250 to 1000 mg/kg b. w.
The EtOAc extract of the aerial part of B. lanata from China [144 ] was investigated for its potential anti-inflammatory activity in 2 animal models: the paw edema induced by carrageenan and egg albumin in rats. The effect of edema reduction after treatment with 100 to 400 mg/kg b. w. of extract was dose-dependent: at the highest dose, there was an effect statistically comparable with the positive reference luteolin (85 ). Another study reported the isolation of apigenin-7-O -β -D-(6″-E -p -coumaroyl)glucopyranoside (139 ), apigenin-7-O -β -D-(2″,6″-E -dicoumaroyl)glucopyranoside (140 ), and verbascoside (227 ) from the ethanolic extract of B. lanata (China) [168 ]. Their anti-inflammatory activity was investigated in the same animal models employed for the plant extract. Compound 227 had a higher anti-inflammatory potential than diclofenac (5 mg/kg b. w.), while the activity of compounds 139 and 140 were similar at 20 – 50 mg/kg b. w. doses. The butanol extract of this species furnished 2 other potentially anti-inflammatory compounds: the flavone C-glycosides panzeroside A (156 ) and B (157 ) [173 ]. A paw edema reduction volume in rats equal to that of 5 mg/kg b. w. of diclofenac was achieved with 30 mg/kg b. w. of both compounds.
Antibacterial activity
Because of the growing human health danger represented by the antibiotics-resistant bacterial strains and in consideration of the therapeutic uses of many species of Ballota and Otostegia genera in microbial related pathologies, as well as in wounds and burns treatments, the antimicrobial activity of EOs, plant parts extracts, and also individual compounds has been extensively investigated. [Table 14 ] reports a synopsis of the most relevant evidence in the literature.
Table 14 Antibacterial activity of Ballota and Otostegia taxa.
Species
Origin
Sample preparation (plant part: solvent)
Test
Target
Ref
B. acetabukosa
Turkey
L: EtOH 80%, W
MIC, MBC (µg/mL): 0.4 – 1.6; 3.2 – 12.5 (EtOH), 0.8 – 3.2, 6.3 – 12.5 W
S. aureus
[249 ]
B. acetabukosa
Turkey
L: EtOH
MIC (mg/mL) 32 – 1024; MBC (µg/mL) 64 – 1024.
E. faecalis, E. coli, P. mirabilis, K. pnemoniae, P. aeruginosa
[250 ]
B. africana
S. Africa
L: MeOH, W
MIC (µg/mL) 438
K. pneumoniae, A. nauplii
[251 ]
B. andreuzziana
Libya
WP: EtOAc, CHCl3 , BuOH, W
AD at 50, 100, 150 mg/mL (mm) 7 – 11
S. aureus, B. subtilis, My phlei
[148 ]
B. deserti
Algeria
EO L: MeOH
MIC biofilm formation EO (µL/mL) 25 – 80; MeOH (mg/mL) 3.25 – 25
S. aureus (ATCC 25923, ATCC 6538-P), S. epidermidis, B. subtilis, B. cereus, S. mutans, M. luteus
[204 ]
B. deserti
Algeria
AP: CH2 Cl2 , MeOH; then isolat. compds: 40, 223, 227
MIC (µM): 46 – 162
E. faecalis, P. aeruginosa, S. aureus
[90 ]
B. inaequidens
Turkey
AP: Ac; isolat. compds: 5, 25, 79, 103, 105, 107, 108, 109
MIC (µg/mL): 25 – 50
S. aureus, B. subtilis, E. coli, P. aeruginosa
[76 ]
B. nigra
Italy
S: EtOH
Quantification of δ -hemolysin. Response in the production of δ -hemolysin, indicating anti-QS activity in a pathogenic MRSA isolate. No inhibitory effect
S. aureus
[252 ]
B. nigra
Pakistan
S, L, R: EtOH then part. between W and n -Hex, EtOAc, CHCl3 , BuOH
AD at 5 mg/mL (mm) 8 – 30
E. coli, S. aureus, P. mirabilis, K. pneumoniae, E. faecalis, S. typhi
[253 ]
B. nigra
Serbia
S, L: EO
MIC (µg/mL): 2.5 – 5
E. coli, S. aureus, B. mycoides, M. lysodeikticus, B. subtilis, K. pneumoniae, C. albicans
[209 ]
B. nigra
France
shoots: 50% EtOH then isolat. compds: 220, 223, 227
MIC (µg/mL): 64 – 128
S. aureus, S. aureus MRSA, P. mirabilis
[187 ]
B. nigra
Italy
WP: W
MIC, dose-dependent biofilm formation inhibition, max inhib. at 128 µg/mL
S. aureus MRSA
[254 ]
B. nigra spp. anatolica
Turkey
L: EtOH
MIC (µg/mL) 250 – 1000
B. subtilis, B. cereus, S. aureus, E. coli, P. vulgaris, S. typhimurium, P. aeruginosa
[255 ]
B. nigra spp. anatolica
Turkey
L: EtOH
AD at 50 µg/mL (mm) 10.0 – 19.2
B. cereus, P. aeruginosa, K. pneumoniae, S. capitis, S. aureus, S. epidermidis, P. acnes, M. nonliquefaciens,
[256 ]
B. nigra ssp foetida
Italy
EO
MIC, MBC (mg/mL): 3 – 7
E. coli, E. cloacae, P. aerouginosa, F. fluorescens, S. aureus, S. epidermidis
[216 ]
B. pseudodictamnus
Pakistan
S, L, R: EtOH then part. bet. W/n -Hex, EtOAc, CHCl3 , BuOH
AD at 2 µg/mL (mm) 0.8 – 20
E. coli, S. aureus, P. mirabilis, K. pneumoniae, E. faecalis, S. typhi
[257 ]
B. pseudodictamnus
Greece
EO
MIC (mg/mL) 0.45 – 10.15, > 20 for E. coli
S. aureus, S. epidermidis, E. coli, E. cloacae, K. pneumoniae, P. aeruginosa
[220 ]
B. rotundifolia
Turkey
WP: MeOH then the extract was split in W-soluble and W-insoluble fractions
MIC (µg/mL): > 72
S. pneumoniae, B. cereus, A. lwoffii, E. coli, K. pneumoniae, C. perfringens
[238 ]
B. saxatilis
Turkey
F: Ac: then isolat. compds: 5, 18, 25
MIC (µg/mL): 25 – 50
S. aureus, S. faecalis, E. coli, P. aeruginosa, K. pneumaniae .
[80 ]
B. saxatilis ssp. brachyodonta
Turkey
EO
MIC (µg/mL): 25 – 50
E. coli, E. feacalis, B. subtilis, S. thyphimurium, S. aureus, S. epidermidis, K. pneumoniae
[221 ]
O. fruticosa
Egypt
EO
MIC (µg/mL): 1.5 – 6
B. subtilis, S. aureus, E. coli, S. epidermidis, S. faecalis, K. aerogenes
[224 ]
O. fruticosa ssp. schimperi
Yemen
EO
MIC (µg/mL): 310 – 1250
B. cereus, S. aureus, E. coli, P. aeruginosa
[222 ]
O. integrifolia
Ethiopia
L: 80% MeOH, CHCl3
MIC (mg/mL): 0.312
M. tuberculosis
[258 ]
O. integrifolia
Ethiopia
EO
MIC (mg/mL) 5 – 100
E. coli 18/9, E. coli ATCC 10536, E. coli CD/99/1, E. coli K88, E. coli RP4, E. coli VC Sonawave 3 : 37 C, P. aeruginosa, S. boydii, S. dysentery, S. flexneri, S. soneii 1, S. soneii BCH 217, V. cholera, B. subtilis
[56 ]
O. limbata
Pakistan
WP: 70% EtOH. then ext. solubilized in DMSO, EtOH, MeOH
MIC (mg/mL): 0.2 – 5
B. subtilis, L. monocytogenes, S. aureus, E. coli, P. aeruginosa, Salmonella spp.
[259 ]
O. persica
Iran
AP: MeOH
MIC (mg/mL): 0.5 – 2
E. coli, B. subtilis, S. aureus, S. epidermis, P. aeruginosa, S. typhi, K. pneumonia, A. niger
[260 ]
O. persica
Iran
AP: 70% EtOH, n -Hex, CHCl3 , MeOH
MIC (mg/mL): 1.25 – 25
E. coli , L. monocytogen, E. faecalis, S. aureus, S. epidermidis, B. subtiltis, P. aeruginosa, Salmonella spp. Klebsiella spp.
[261 ]
The MIC and MBC values may vary over a wide interval and are often lower in order of magnitude than that of the antibiotics selected as positive controls. For example, the ethanolic extract of B. acetabulosa was found to be significantly active against Escherichia coli with MIC and MBC values equal to ampicillin (32, and 64 µg/mL, respectively). On the contrary, the same extract was inactive against Pseudomonas aeruginosa (Schroeter) Migula (MIC and MBC 1.02 mg/mL) [250 ].
The direct relation between the chemical composition of the plant extracts and the antimicrobial activity is not always clearly explainable. Sometimes this relation is evident as in the case of the antimicrobial activity of B. pseudodictamnus EO from Greece [220 ]. Its antibiotic activity was assessed against a panel of 6 pathogenic microorganisms, along with the activity of the principal components of the oil individuated by GC-MS. Unsurprisingly, the MIC of the main component caryophillene oxide (from 0.07 to 5.20 µg/mL) was from 4 to 6 times stronger than that of the whole oil, in which caryophillene oxide is present at 22.8%! The author stated, “The antibacterial property of the oil is suspected to be associated with the high percentage of caryophyllene oxide which is known to possess strong antibacterial activity.” The data reported seem to show any other component of this EO as a mere diluent! This is just an example of how the rather vague postulate of the “synergistic effect” of natural product mixtures in biological systems should at least be deeply questioned. Another example of antibacterial activity of a caryophillene rich EO is in the study of B. saxatilis subsp. brachyodonta from Turkey [221 ]. A MIC of 50 µg/mL against all of the bacterial genera evaluated was found; caryophillene, caryophillene oxide, and epi -bicyclosesquiphellandrene were the main components of the oil.
An example of the lack of a molecular explanation is the study of the activity of the water and methanolic extract of leaves of B. africana from South Africa [251 ]: MICs were reported at 438 and 370 µg/mL versus Klebsiella pneumoniae (Schroeter) Trevisan for methanolic and water extracts, respectively. However, an attempt to isolate single bioactive compounds using bio-guided fractionation of the crude extract failed. Additionally, the authors underlined, for not so evident reasons, the apparent discrepancy between the observed antibacterial activity and the lack of resveratrol in the extract. Furthermore, in one case, the demonstration of the lack of antibacterial activity versus Propionicbacterium acnes (Gilchrist) Douglas of Italian B. nigra extract demonstrated the inconsistency of its claimed ethnopharmacological use as an anti-acne remedy [262 ]. In some cases, the antimicrobial activity of a certain species may vary greatly depending on the extraction solvent and the target investigated. A clear example is the case of the aerial part of Iranian O. persica
[261 ]: the initial ethanolic extract was portioned in fractions soluble in n -Hex, CHCl3 , and finally MeOH. The lowest MIC and MBC values were 1.25 mg/mL for the CHCl3 ext. against Staphylococcus aureus and Enterococcus faecalis , while the MeOH extc. had a 25 mg/mL MIC for Listeria monocytogens (E. Murray et al.) Pirie. Finally, the bacteria species E. coli, P. aeruginosa, Salmonella spp., Klebsiella spp., and Proteus spp. were completely insensitive to all of the extracts.
In other cases, the different geographical origin of the plant material may be the cause of strong differences in the bioactivity, as is the case of the EO derived from O. fruticosa . The plant harvested in Egypt is characterized by antibacterial activity with MIC in the order of µg/mL [224 ]. On the contrary, the same taxa from Yemen show MIC values 2 order of magnitude higher than the former [222 ]. This difference can be explained by the deep difference in the composition of the 2 oils, regarding their main components in particular, as discussed above.
[Table 14 ] includes cases where the crude extracts were further portioned with organic solvents in the attempt to concentrate the more active compounds [253 ], [254 ], [257 ], as well as other cases, where the bioactivity was evaluated for single isolated molecules [76 ], [89 ], [187 ]. In most, highly polar extracts obtained with protic solvents and water contained mainly phenols, phenylpropanoids, and glycosylates. Only 1 case reports the isolation and the measurement of the antibacterial activity of terpenes from the Ac extract of B. saxatilis subsp. saxatilis from Turkey [80 ].
Antifungal activity
The general points discussed for antibacterial activity are valid also in this case, as methodological approaches are obviously similar and a lot of investigations involve both bacterial and fungal species as targets. [Table 15 ] summarizes the most relevant data reviewed.
Table 15 Antifungal activity of Ballota and Otostegia taxa.
Species
Origin
Sample preparation (plant part: solvent)
Test
Target
Ref.
B. acetabulosa
Turkey
L, R: 50% EtOH
MIC (mg/mL) 1.56 – 25.0
C. albicans, C. tropicalis, C. guilliermondii, Cryptococcus neoformans, C. laurentii
[263 ]
B. deserti
Algeria
EO L: MeOH
MIC biofilm formation EO (µL/mL) 25; MeOH extr, (mg/mL) 12.5
C. albicans
[204 ]
B. inaequidens
Turkey
AP: Ac; then isolat. compds: 5, 25, 79, 103, 105, 107, 108, 109
MIC (µg/mL) 3.1 – 12.5
C. albicans, C. crusei
[76 ]
B. nigra
Paikstan
S, L, R: EtOH then partition between W/n-Hex, EtOAc, CHCl3 , BuOH
Agar tube diluition, results reported as inhibition “positive” or “negative”; crude extrat always positive at 2 mg/mL
A. niger, A. flavus, A. fumigatus, F. solani
[253 ]
B. nigra ssp. anatolica
Turkey
L: EtOH
MIC (µg/mL): 500 – 1000
C. albicans, D. hansenii, K. fragilis, R. rubra
[255 ]
B. nigra ssp foetida
Italy
EO
MIC (mg/mL) 5.5; MBC (mg/mL) 15.0
C. albicans, C. glabrata, C. tropicalis
[216 ]
B. pseudodictamnus
Pakistan
S, L, R: EtOH then partition between W/n -Hex, EtOAc, CHCl3 , BuOH
Agar tube dilution, results reported as inhibition “positive” or “negative” at 2 mg/mL
A. niger; A. fumigates, A. flavus; F. solani
[257 ]
B. pseudodictamnus
Greece
EO
Not active
C. albicans, C. tropicalis, C. glabrata
[220 ]
B. rotundifolia
Turkey
WP: MeOH then the extract was split in W-soluble and W-insoluble fractions
MIC (µg/mL) > 72
C. albicans, C. krusei
[238 ]
B. saxatilis
Turkey
L: Ac then isolat. compds: 5 , 18, 25
Agar diluititon MIC (µg/mL): 1.5 – 3.1
C. albicans
[80 ]
B. saxatilis ssp. brachyodonta
Turkey
EO
MIC (µg/mL): 25
C. albicans (clinic strain), C. parapsilosis
[221 ]
B. undulata
Egypt
L, S, F: EtOH, EtOAc, CHCl3 , n -Hex
MIC (mg/mL): 25 – > 150
T. rubrum, T. tonsurans, C. albicans, C. tropicum, P. lilacinus, P. variotii, S. bervicaulis
[264 ]
O. integrifolia
Ethiopia
EO
MIC (µg/mL): 50 – 100
A. niger, C. albicans, P. funiculosum, P. notatum
[56 ]
O. fruticosa
Egypt
EO
MIC (µg/mL): 6.0, 16.0
C. albicans, S. cerevisiae
[224 ]
O. limbata
Paikstan
WP: MeOH, then partition in n -Hex, CHCl3 , EtOAc, BuOH
MIC (mg/mL) 0.18 – 1.5
S. setubal, P. pickettii, S. aureus, M. luteus
[265 ]
O. persica
Iran
AP: MeOH
MIC (mg/mL): 1.0
C. albicans
[260 ]
Antitumor activity
The EO of Ballota undulate, B. saxatilis , and B. nigra collected in Italy were assessed for their in vitro cytotoxicity toward the Hep-G2 hepatocarcinoma and MCF-7 breast carcinoma cell lines [218 ]. The 3 oils showed moderate inhibition values against the former target (IC50 : 54.7, 65.4, and 69.9 µg/mL, respectively) and low values for latter (> 100 µM). Sesquiterpenes were found as major components in the oils. The MCF-7 cells were instead more sensitive to the EO of O. fruticosa with an IC50 of 55.1 µg/mL. This taxon was moderately active also against MDA-MB-231 cells with IC50 of 72.3 µg/mL [222 ].
Rhabdomyosarcoma cells were found to be sensitive to treatment with the methanolic extract of O. limbata from Pakistan [266 ]. Cell viability tests showed up to 93% mortality after 72 h, a higher level than cisplatin (23%).
The EtOH extract of AP of B. cinerea was assessed for its cytotoxicity against several cancer cell lines, showing moderate activity [267 ] with the following LC50 values (µg/mL): 131.8 for SK-MEL 2, 275.4 for BE (2) C, and 302.0 for U87MG.
Precalyone (45 ) was isolated, together with other diterpenes, from the ethanolic extract of the leaves and the stems of B. cinerea
[86 ]. This compound was active against the murine P-388 lymphocytic leukemia.
Docking studies of 18 phyto compounds from the plant B. nigra of the family Lamiaceae were carried out. From the results, ballotinone (10 ), and ballonigrin (5 ) were found to have the best binding efficiency with the active site residues of the protein. The binding energies of the 2 compounds were − 12.0691 kcal/mol and − 10.2564 kcal/mol, respectively. This study provides a promising anti-cervical cancer inhibitor for further drug development [268 ].
7α -Acetoxyroyleanone (73 ), also occurring in B. nigra
[115 ], was shown to be an active anticancer agent against both MIAPaCa-2 and melanoma (MV-3) cancer cell lines (IC50 = 4.7 and 7.4 µg/mL, respectively) [269 ]. Additionally, it also exhibited cytotoxic activity against 5 more human cancer cell lines including, breast (MCF-7), human leukemia (CEM and HL-60), murine skin (B16), and colon cancer (HCT-8) cell lines in the range of IC50 = 0.9 – 7.6 µg/mL. Its cytotoxic activity seemed to be related to inhibition of DNA synthesis [270 ].
The anticancer activity of marrubenol (36 ), present in B. pseudodictamnus
[79 ], against osteosarcoma cells along with evaluating its effects on autophagic cell death, reactive oxygen species generation and cell migration and invasion tendency was evaluated. The results indicated that compound 36 exhibited an IC50 value of 45 µM and exerted its cytotoxic effects in a dose-dependent manner. Moreover, it was observed that the drug inhibited colony formation and induced autophagy dose-dependent [271 ].
A moderate antiproliferative effect on lung adenocarcinoma cell line (H1975 and XLA-07) and mouse mononuclear macrophage leukemia cell line (RAW264.7) was detected for leoheterin (34 ) [272 ], a labdane diterpenoid occurring in B. aucheri
[71 ], [74 ], [97 ] and O. fruticosa
[82 ], [98 ].
Other bioactivities in vitro
The efficacy of B. nigra in its traditional neurosedative use was proven by assessing the binding activity in dopaminergic, benzodiazepine, and morphinic receptors of a number of compounds isolated from the leaf extract in EtOH/H2 O 1 : 1 [236 ]: (+)-(E )-caffeoyl-L-malic acid (186 ), verbascoside (227 ), forsythoside B (223 ), arenarioside (220 ), and ballotetroside (221 ). These molecules were active with IC50 values ranging from 0.4 to 10 µM.
The labdane diterpene cinereanoid D (16 ), the flavonoid glycosides isoquercetin (122 ), nicotiflorin (133 ), and martynoside (226 ) isolated from the aerial part extract (95% EtOH) of B. cinerea, collected in India [88 ], significantly inhibited the ATP binding of a tumor growth-promoting heat shock protein, Hsp90. No significant binding inhibition was revealed for the protein Hsp70.
B. nigra
[253 ] and B. pseudodictamnus
[257 ] from Pakistan were evaluated by the same group for their anti-leishmanial activity. The extracts of the plant stems, leaves, and roots in EtOH were partitioned between water and several organic solvents: n -Hex, EtOAc, chloroform, and n -BuOH. The single subfractions of the 2 species showed the ability to inhibit the parasite development process at various stages.
The leaves of B. deserti harvested in Tunisia were extracted in a solvent of increasing polarity [25 ]. MeOH, BuOH, and EtOAc extracts showed significant antiviral activity against coxsakie B3 virus with IC50 values ranging from 100 to 135 µg/mL and with a selective index above 3.
The genotoxic and antigenotoxic activities of some B. deserti AP extracts in various solvents were evaluated on E. coli PQ37 cells by the SOS Chromotest. Additionally, a number of pure compounds isolated from the same plant were included in this investigation [89 ]. EtOAc, MeOH, and BuOH extracts proved moderately to highly genotoxic in a dose-dependent manner, while apigenin-7-O -β -neohesperidoside (114 ), verbascoside (227 ), apigenin-7-O -β -D-glucopyranoside (112 ), and apigenin (76 ) resulted as marginally genotoxic. Furthermore, the protective effect of all extracts and the isolated compounds was studied on nitrofurantoin (NF) induced damage. MeOH, EtOAc, and BuOH extracts significantly decreased the induction factor of NF by 89.8%, 94.3%, and 96.2%, respectively, while compounds 76, 112 –114, 139, 223, and 227 decreased the genotoxicity by a factor of 65 to 97%.
Insecticidal activity
The hot water extract of the leaves of B. undulata from Jordan [273 ] was effective as a repellent agent against the sweet potato parasite Bemisia tabaci (Gennadius). A set of tomato leaves treated with the extract was compared with untreated tomato leaves, demonstrating a significant difference (analysis of variance test) in the number of insects that attacked each group. The same taxon, in the form of leaves brewed in hot water, showed acaricidal activity against the spider mite Tetranychus urticae . A high mortality (53%) was achieved by treating adults with the extract, while the eggs remained unaffected [274 ].
An insect repellent activity was also disclosed for O. integrifolia from Ethiopia [275 ]. The headspace of fresh leaves, dried leaves, and burned dried leaves were evaluated giving a repellent ratio of 29 – 56% in an elegant experimental setting developed by the author. This activity was shown to be associated with the presence of β -ocimene in the headspace blend.
The n -Hex extract of O. limbata (Pakistan) showed larvicidal activity against the banana parasite Drosophila melanogaster Meigen [276 ]. The crude solid extract was mixed with overripe banana at 2 – 6% w/w and larvae were let to feed. A concentration dependent mortality from 12 to 89% was observed. Also, a pupation reduction effect was established with a ratio of development dropping from 88 to 23%, depending on the extract concentration (0.5 – 2.0%).
The Ac extract of O. persica leaves collected in Iran [277 ] was studied as a pesticide against Aphis fabae Scopoli, Aphis gossypii Glov., Myzus persicae Sulzer, and Tribolium castaneum Herbst. The maximum mortalities of individuals after 48 h of treatment at 80 µL/mL dose of extract were 55, 58, 88, and 34%, respectively. The prolongation of exposure time to 60 h did not appear to significantly improve the mortality rate.
An Ac extract of B. hirsuta (AP) from Spain [278 ] caused a significant growth inhibition in the T. castaneum larvae (29%) accompanied by 20% mortality. This effect was related to the harvesting time, as the plant taken in November was active, while a sample collected in April was inactive.
Effects on central nervous system
The extract of B. limbata (Pakistan) leaves in n -buthanol was active as antitussive by reducing the SO2 -induced cough in mice. The treatment of animals with 800 mg/kg b. w. of the extract caused the cough episodes to be reduced from 46 to 12 in 60 min; an efficacy analogous to that of the standard antitussive drugs codeine and dextromethorphan [279 ]. The toxicity test proved the extract to be inoffensive until the dose of 5000 mg/g b. w.
The brew (in hot water) of the AP of B. nigra subsp. anatolica from Turkey possess both antidepressant and anxiolytic activities in rats, determined with the forced swimming and the elevated plus-maze tests [280 ].
Two extracts of O. persica harvested in Iran were prepared by suspending the AP of the plant in n -Hex and 80% EtOH and were tested for the alleviating effect in the opioid withdrawal syndrome [281 ], as this is a traditional use in Iran folk medicine. Male mice were intoxicated with morphine and the withdrawal signs (jumping, rearing, diarrhea, piloerection, tremor, and ptosis) were recorded after injection of naloxone in untreated animals, treated with clonidine (0.2 mg/kg b. w.) and with increasing doses of both the extracts (500 – 1500 mg/kg b. w.). All of the clinical signs were reduced significantly to levels comparable to those obtained with clonidine at the maximum dose of utilized ethanolic extract. The n -Hex extract, however, was only able to reduce diarrhea.
An anticonvulsant effect was evidenced in the MeOH extract of O. persica
[282 ], sustaining the traditional use of this plant in seizure management with scientific evidence. Convulsions were induced in mice with pentylenetetrazole, followed by an IP injection of 800 mg/kg b. w. which had a 93% protective effect, comparable to that of benzodiazepine.
The extract of AP of B. glandulosissima in water were investigated for their antinociceptive activity in mice by acetic acid-induced “writhing” and “tail-flick” tests [283 ]. A lethal dose of 8.85 g/kg b. w. was determined. The extract, intraperitoneally administrated at 100 and 200 mg/kg b. w. doses, had promising antinociceptive activity, comparable to acetylsalicylic acid, utilized as a positive control.
An analgesic effect similar to paracetamol was obtained with the MeOH extract of B. deserti AP from Algeria [248 ] when administrated at 400 mg/kg b. w. to albino rats. Acetic acid induced abdominal writhes were reduced by 73%.
Metabolism control effects
The extract of B. nigra from Jordan, obtained by brewing the AP in EtOH water 7 : 3, was proven to possess very interesting hypoglycemic activity both in healthy and in allossana-induced diabetic albino rats. After a single dose treatment of 400 mg/g b. w., the first group had a glucose blood concentration reduction from 96 to 62 mg/dL after 6 h, while for the second group, the value dropped from 324 to 271 mg/dL [284 ]. In another study by the same authors [285 ], an analogous B. nigra preparation administrated at the same dose for 7 d was also effective in reducing hematic cholesterol (from 193 to 144 mg/dL), triglycerides (from 97 to 83 mg/dL), and CK protein (from 431 to 348 IU/L).
Similar hypolipidemic effects were observed in rabbits treated with the AP extract, in EtOH/water 7 : 3, of B. undulata from Jordan [286 ]. Two animal groups were treated with 400 mg of cholesterol/kg b. w. per day dissolved in 5 mL of coconut oil for 120 d; in 1 group 1.2 g/kg b. w. per day of the extract were added to the diet. A strong difference in lipidemic parameters was observed for the 2 groups: total cholesterol 807 versus 104, HDL 246 versus 40, phospholipids 252 versus 112, triglycerides 259 versus 74. Despite their scientific relevance, in our opinion, the eventual transfer of these findings on human trials might be strongly inhibited by the evident difficulty to orally administer a dose of 84 g/day of plant extract to an average weight human.
A potent antidiabetic effect was identified for the ethanolic extract of the O. persica AP from Iran [287 ]. The hematic glucose level of STZ-induced diabetic rats was normalized when animals received 200 to 500 mg/kg b. w. per day of the extract. At the maximum dose, the glucose level was lowered from 405 – 420 to 170 – 230 mg/dL, depending on the control time. Similar results were obtained by other authors [288 ] with the methanolic extract of this plant, also collected in Iran. In this case, the glucose level reduction observed in rat blood was accompanied by an increase in the insulin secretion in C187 pancreatic β -cells. Additionally, a reduction in lipidic oxidation was proved by the decrease in MDA values and the increase in GSH values. The ethanolic extract of O. persica was demonstrated to possess protective effects by preventing renal damage induced by ischemia/reperfusion induced in diabetic rats [289 ]. The hematic renal function indicators were ameliorated after treatment with 300 mg/kg b. w. of the extract for 2 wk: urea from 67.6 to 36.1, creatinine from 2.32 to 1.32, glucose from 378.6 to 147.2. Furthermore, kidney resection and tissue evaluation of the oxidative stress parameters (MDA, MPO, NO, SOD, and CAT) evidenced a beneficial effect in O. persica treated animals.
The antidiabetic activity of the AP of O. persica were assessed in different factions of the crude extract, following their solubility in PE, CHCl3 , EtOAc, n -BuOH, and MeOH [141 ]. The antioxidant activity was measured by DPPH method and was correlated to antidiabetic activity in mice. MeOH extract was effective in reducing hematic glucose with a 300 mg/kg b. w. dose. Both MeOH an EtOAc extract showed antioxidant activity with IC50 of 0.49 mg/mL for both. Finally, 4 compounds were isolated from the active extracts: chrysoeriol (91 ) from EtOAc, 6-methylapigenin (77 ), apigenin-7-O -β -D-glucopyranoside (112 ), and echinaticin (138 ) from the MeOH one.
The aerial part of O. persica also showed a potent antidiabetic effect when extracted in water at 40 °C [290 ]. Fasting blood sugar, insulin, and HOMA.IR (homeostasis model assessments for insulin resistance) were evaluated in STZ-induced diabetic mice after 10, 20, and 30 d of administration of up to 400 mg/kg b. w. of the extract. The indicator improvements were comparable to those reported in other studies, and total cholesterol and triglycerides were also significantly reduced: 95 versus 75 mg/dL for the former and 203 versus 71 for the latter. A reduction in the number and the mass of pancreatic β -cells was evidenced by histopathological visualization techniques.
The antidiabetic effect of O. persica (AP extracted in EtOH/H2 O 1 : 1) was also studied by stereological analysis of pancreas tissue in diabetic (STZ induction) Sprague-Dawley rats [291 ]. The oral administration of 500 mg/kg b. w. reduced blood glucose levels and insulin production, as reported in many other references. After 1 mo, the animals were sacrificed and pancreatic tissue was analyzed. A hypertrophic change in the remaining β -cells of the diabetic group was observed, accompanied by a reduction in pancreatic islet volume. These phenomena were significantly reduced in the animals treated with the extract.
B. aucheri extract (AP in 70% MeOH) [232 ] was effective in reducing postprandial hematic glucose increment in type II diabetic rats, while it was ineffective in type I diabetic animals. This antidiabetic activity was associated with a notable antioxidant activity determined in this sample ([Table 13 ]).
Bone damage such as osteoporosis may constitute an important comorbidity in patients affected by mellitus diabetes. The aqueous extract of O. persica (Iran) was proven to act as a protection from bone damage in STZ treated diabetic rats [292 ]. Rats was treated orally with 200 – 450 mg/kg b. w. for 29 d. Then the left femoral and tibiofibular bones were dissected and evaluated histomorphometrically, while the right-side bones were removed for ash weight determination. The plant extract was able to significantly reverse the epiphyseal and metaphyseal trabecular width reduction observed in untreated animals. Additionally, the epiphyseal bone area/tissue were normalized with the utilization of the minimum extract dose. Ash weight was significantly lower in animals treated at 450 mg dose.
The Ethiopian plant O. integrifolia , in particular the leaf extract in 80% MeOH, was also effective in contrasting diabetes in mice and rats [293 ]. STZ induced diabetic mice receiving doses from 100 to 400 mg/kg b. w. of extract displayed time-dependent hypoglycemic effects. After 4 h, the dose of 200 mg reduced the hematic glucose from 375 to 159 mg/dL.
Three extracts were obtained from the AP of B. cinerea (India) by brewing them in PE, EtOAc, and MeOH [294 ]. They were active in reducing blood glucose levels in diabetic rats both with acute (after 4 h) and with chronic (after 21 d) alloxana-induced disease. Total reduction of glucose reached values (36 – 42%) comparable to the standard antidiabetic drug glibenclamide. Other authors [267 ] reported slightly different values for the activity of the same extracts, together with the EtOH extract, assessed in the reduction of hematic glucose in STZ-induced diabetic rats: from 18.2 to 23.4% reduction after 24 h at 100 mg/kg b. w. Furthermore, similar results in reducing murine glycemia were obtained with the butanol and water extract of the same taxon [233 ]. In the last study, the lipidic profile, the hepatic glycogen content and the pancreatic parameters (SOD, GSH, and PGx) were also evaluated after 15 d of extract treatments at 50 mg/kg b. w. Almost all of the parameters were normalized as compared to standard values, and these beneficial effects were confirmed by the histopathological evaluation of pancreatic and hepatic tissue dissections. Take note that the dose of extract implemented in this study is significantly lower than the average doses normally utilized in similar contexts.
4-Methoxybenzo[b ]azet-2(1H)-one (214 ) and 3β -hydroxy-35-(cyclohexyl-5′-propan-7′-one)-33-ethyl-34-methyl-bacteriohop-16-ene (178 ), isolated from the aerial part of B. cinerea (India) [182 ], significantly reduced the blood glucose level in alloxan-induced diabetic rats at the dose of 10 mg/kg b. w. administered orally.
An interesting protective effect for hyperlipidemia was investigated for the aqueous extract of B. arabica (syn. L. urticifolia ) in a Triton WR-1339 induced hyperlipidemic rat model [295 ]. The administration of a 100 – 400 mg/kg b. w. dose for 24 h was able to restore normal values of plasma lipidic parameters, total cholesterol, TG, LDL, VLDL, and to significantly raise the HDL level.
Other bioactivities in vivo
The fruit extract of B. undulata obtained in EtOH/water 7 : 3 was shown to be effective as a fertility controller in Albino rats [296 ]. The effects were time dependent: after a treatment of 4 wk at 15 mg/kg b. w. per day, the numbers of pregnancies was not reduced significantly, while only a slight reduction in embryo and ovarian weights was observed. On the contrary, when the treatment was prolonged until 12 wk, the percentage of embryo implantation and pregnancies with respect to the controls was statistically relevant.
The extract of O. persica AP in MeOH was tested for its healing promoting activity in the skin of Wistar rats [297 ]. The healing process of burns provoked in the dorsal part of animals was accelerated by the application of a ointment in which the extract was dispersed. The histological evaluation evidenced an increase in fibroblast proliferation, angiogenesis and re-epithelialization that improved in a 5- to 14-d time range.
Ischemia-reperfusion is a dangerous syndrome that can cause severe injuries to remote organs, due to multiple effects, including the increase in reactive oxygenated radicals and general inflammation conditions. The ethanolic extract of O. persica (Iran) demonstrated protective effects toward renal injury in rats suffering of a hindlimb ischemia reperfusion surgically induced by clamping the femoral artery [298 ]. Reperfusion induced kidney damage including the increase of water uptake, creatinine excretion rate, and kidney/body weight. The animals treated with 300 mg/kg b. w. of extract 2 d before intervention had the above-mentioned renal functionality parameters at levels comparable with the negative control.
The antihypertensive effect of the AP extract (70% EtOH) of O. persica was proven in Wistar rats suffering from dexamethasone-induced hypertension [299 ]. The systolic pressure increase from 115 to 143 mmHg was completely suppressed by a dose of 400 mg/kg b. w. per day of the extract, administered 2 d before starting the dexamethasone treatment. The hematic H2 O2 and FRAP values increased by dexamethasone were also normalized in the animals receiving the plant brew.
Anti-malarial activity
A significant antimalarial effect in Plasmodium berghei Vinke & Lips-infected mice was disclosed for O. integrifolia collected in Ethiopia [300 ]. Mice were administrated with 200, 400, and 800 mg/kg b. w. doses of the leaf extract prepared in chloroform, MeOH, and water. The survival of the animals treated with polar extract was dose-dependent and significantly higher than that of the negative control (10.5 vs. 7.5 d of H2 O ext. at max. dose; 13.5 vs. 7 for MeOH ext.). General lack of toxicity was proven until reaching a 2000 mg/kg b. w. dose. Also in this case, the very high dose of extract employed may be an obstacle for possible developments toward application to humans. However, a study appearing in the literature in the same year [101 ] resulted in the bio-guided isolation of the labdane diterpene otostegindiol (43 ) from the methanolic extract of O. integrifolia leaves. This compound showed chemosuppressive properties against P. berghei with a maximum suppression ratio of 73.16% at 100 mg/kg. Additionally, the EtOH extract of the aerial part of Iranian O. persica
[301 ] demonstrated antimalarial activity in P. berghei infected mice with ED50 of 45 mg/kg b. w. Furthermore, an interesting synergistic effect was observed when CQ-sensitive animals were treated with a combination of this drug and the plant extract; for example, a combination of 70% of the ED50 of CQ with 30% of extract caused a 26% increase in the mean effective dose.
The effective antiprotozoal activity against another relevant malarial parasite, Plasmodium falciparum Welch, was disclosed in both the PE and the chloroform extracts of B. cinerea from India [302 ]. The IC50 values for the 2 materials were 4.39 and 1.84 µg/mL, respectively.
Hepatoprotective effects
The AP extract (80% MeOH) of O. persica collected in Iran [303 ] was effective in strongly reducing liver damage in CCl4 intoxicated rats (2.5 mg/kg b. w.). After administrating 400 mg/kg b. w. of extract, hematic liver damage parameters were significantly ameliorated with respect to untreated animals: ALT 13%, AST 11.6%, plasma MDA 6.7%, liver MDA 11.4%, liver GSH + 21%. Histological evaluation of liver sections demonstrated the prevention of tissue degradation in treated rats. A similar protective effect was described for the aqueous extract of the AP of B. glandulosissima from Turkey [245 ]. Liver damage indicators in the blood of CCl4 -treated rats (0.8 mL/kg b. w.) were reduced significantly with 100 mg/kg b. w. of extract: AST 59%, ALT 47%, ALP 43%, bilirubin 47%. The efficacies of the 2 above-cited treatments are hardly comparable considering the differences in applied dose intoxication, in the extraction procedure and in the feeding procedures of the animals involved in these studies. Similar protective effects on the liver were found in the AP extract (70% MeOH) of O. persica collected in Iran [304 ] administrated to rats at 80 – 120 mg/kg b. w.
The hepatoprotective effect of B. cinerea (syn. Roylea elegans Wall. ex Benth.) collected in India was assessed for the AP extract obtained in EtOH/H2 O 1 : 1 in a CCl4 and paracetamol toxicity induced model in rats [305 ]. Hepatic damage was evaluated by several blood parameters, such as SGOT, SGPT, ALP, and TB, after treatment with 100 – 400 mg/kg b. w. of extract for 7 d. Also, the liver oxidative stress indicators GSH and TBARS were followed. The pathological displacement of all of the indicators was reduced in a dose-dependent fashion and complete normalization occurred at the dose of 400 mg.
Enzymatic activity modulation
There are a number of investigations aimed at evaluating the plants from Ballota and Otostegia genera as a source of useful bioactive compounds isolated from the complex blend of their secondary metabolites. The extract of O. limbata root in MeOH led to the isolation and identification of 3 new tricyclic cis -clerodane diterpenes: limbatolide A (64 ), limbatolide B (65 ), and limbatolide C (66 ) that were assessed of their inhibitory potential against acetylcholinesterase (AChE; EC 3.1.1.7) and butyrylcholinesterase (BChE; EC 3.1.1.8). The inhibition activity was higher for the latter enzyme (IC50 22.3, 17.5 and 14.2 µM, respectively) than for the former (IC50 38.5, 47.2, and 103.7 µM) [112 ]. These enzymatic systems were also the targets of another similar work concerning the isolation of 6 clerodane tricyclic diterpenes from the chloroform extract of B. limbata
[107 ]: ballatenolide A (59 ), 15-methoxypatagonic acid (71 ), patagonic acid (72 ), and limbatenolides A – C (54 –56 ). All of the compounds showed inhibitory activity with BChE with IC50 values ranging from 24.9 to 51.0 µM, lower than the standard inhibitor galanthamine (8.5 µM). The first group of 3 compounds also showed a moderate inhibitory activity with AChE with IC50 values between 50.0 and 102 mM (galanthamine 0.50 µM). The inhibition activity toward the 2 enzymatic systems was also assessed with the MeOH extract of the aerial part of B. deserti from Algeria [204 ], obtaining moderate IC50 values: 277.4 µg/mL for AChE and 93.3 µg/mL for BChE.
Ballotenic acid (60 ) and ballodiolic acid (62 ) were isolated from the chloroform soluble fraction of the MeOH extract of B. limbata
[110 ] and displayed inhibitory potential against lipoxygenase enzyme in a concentration-dependent fashion with IC50 values of 99.6 µM and 38.3 µM, respectively.
Furthermore, the crude n -Hex extract of B. nigra subsp. kurdica
[306 ] from Iran was investigated for its possible tyrosinase inhibitory activity by the colorimetric Tyrosinase inhibition assay (IC50 = 3.67 µg/mL); however, no attempt was made to isolate individual active molecules.
Tyrosinase was also effectively inhibited by 2 compounds isolated from the AP extract of B. cinerea from India [182 ]: 4-methoxybenzo[b ]azet-2(1H)-one (214 ) and 3β -hydroxy-35-(cyclohexyl-5′-propan-7′-one)-33-ethyl-34-methyl-bacteriohop-16-ene (178 ) with inhibition rate of 83.0 and 58.2%, respectively, at 100 µM. These compounds were also effective inhibitors of α -glucosidase (78.5% and 58.4%). This inhibitory activity is related to the above discussed antidiabetic activity in vivo of these compounds. The α -glucosidase and β -glucosidase reduction activities were evaluated in a study on the antidiabetic activity in vitro and in vivo of some extracts of B. cinerea from India [294 ]. Three fractions were found more active, respectively, obtained with PE, EtOAc, and MeOH; their activity reduction power ranged about from 55 to 80%, with the MeOH extract being the most active. In another work [267 ], these extracts were tested in an in vitro inhibitory activity test against protein tyrosine phosphatase-1B, showing results ranging from 39 to 65% inhibition at 100 µM.
α -Amylase, an enzyme involved in saccharide metabolism which is believed to possess preventive properties for type II diabetes, is strongly inhibited by polyphenolic compounds [307 ]. For this reason, the inhibitory activity of the extracts of O. persica was evaluated in association with the antioxidant activity (DPPH test, see [Table 13 ]) [243 ]. The initial crude extract obtained in EtOH was then partitioned in solvents of different polarities: PE, EtOAc, CHCl3 , n -BuOH, EtOH. The enzymatic parameters were measured for all of the fractions: inhibition rate from 53.3 (pet. Ether) to 99.4% (EtOAc). The author attempted to relate the total phenolic content of the extracts both with the antioxidant and the enzyme inhibition activity.
The 2 new flavonoidal glucosides leufolins A (163 ) and B (152 ) isolated from B. arabica (syn. L. urticifolia ) have shown to be potent inhibitors of BChE enzyme (IC50 values 1.6 and 3.6 µM, respectively) when compared to serine, used as a positive control (IC50 0.93 µM). On the other hand, very weak activity was observed against acetylcholinestrase (IC50 values 74.5 and 72.3 µM, respectively), compared to eserine (IC50 = 0.04 µM) [171 ]. The new steroid leucisterol (179 ), also isolated from the same species, showed potent inhibitory activity against butyrylcholinesterase enzyme (IC50 = 3.2 µM). [181 ].