Moderate-intensity Exercise Training in Sole and Simultaneous Forms with Insulin Ameliorates the Experimental Type 1 Diabetes-induced Intrinsic Apoptosis in Testicular Tissue

Zahra Samadian; Javad Tolouei Azar; Sana Moshari; Mazdak Razi; Asghar Tofighi

doi:10.1055/a-0985-4332

International Journal of Sports Medicine, Table of Contents

Int J Sports Med 2019; 40(14): 909-920
DOI: 10.1055/a-0985-4332

Genetics & Molecular Biology

Moderate-intensity Exercise Training in Sole and Simultaneous Forms with Insulin Ameliorates the Experimental Type 1 Diabetes-induced Intrinsic Apoptosis in Testicular Tissue

Zahra Samadian

²Sama Technical and Vocational Training College, Islamic Azad University, Urmia Branch, Urmia, Iran

,

Javad Tolouei Azar

¹Department of Exercise Physiology and Corrective Exercises, Faculty of Sport Sciences, Urmia University, Urmia, Iran

,

Sana Moshari

³Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

,

Mazdak Razi

³Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

,

Asghar Tofighi

¹Department of Exercise Physiology and Corrective Exercises, Faculty of Sport Sciences, Urmia University, Urmia, Iran

› Author Affiliations

Abstract

The aim of this study was to investigate the ameliorative effect of moderate-intensity exercise training in sole and simultaneous forms with insulin on experimental type 1 diabetes (T1D)-induced apoptosis. A total of 36 mature male Wistar rats were divided into six equally sized groups, including sedentary control (Con), moderate-intensity exercise training (E-sole), sedentary T1D-induced (D-sole), moderate-exercise-trained T1D-induced (DE), insulin-treated sedentary T1D-induced (DI) and exercise-trained, and insulin-treated T1D-induced (DEI) groups. The 6-week exercise training intervention was involved 30 min of moderate-intensity running on a treadmill once daily (5 days/week). Next, tubular differentiation (TDI) and spermiogenesis (SPI) indices were assessed. The Bcl-2, Bax and caspase-3 expressions were determined using RT-PCR, immunohistochemistry and western blot techniques. Finally, the TUNEL staining was used to analyze the apoptosis ratio. The moderate-intensity exercise training in the sole and when simultaneously considered with insulin (DEI) maintained testicular cellularity, up-regulated Bcl-2 expression, reduced Bax expression and ameliorated the diabetes-induced apoptosis. We failed to show remarkable alterations in caspase-3 mRNA and protein levels in the DE group versus D-sole animals. In conclusion, the moderate-intensity exercise training is able to potentially protect testicular cells from T1D-induced intrinsic apoptosis via up-regulating Bcl-2 and downregulating Bax expressions. Moreover, it amplifies the insulin-induced anti-apoptotic impacts.

Key words

spermatogenesis - moderate-intensity exercise training - type 1 diabetes - apoptosis

Full Text

References

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