Eglerisine, a Novel Sesquiterpenoid Tropolone from Dulacia egleri with Antiproliferative Effect against an Acute Myeloid Leukemia Lineage

Leice M. R. de Novais; Luiz F. Ferreira; Paulo T. de Sousa Jr.; Tereza A. N. Ribeiro; Marcos J. Jacinto; Carlos H. C. dos Santos; Mário G. de Carvalho; Heron F. V. Torquato; Edgar J. Paredes-Gamero; Virgínia C. P. Silva

doi:10.1055/a-1021-0611

Planta Medica, Table of Contents

Planta Med 2020; 86(01): 55-60
DOI: 10.1055/a-1021-0611

Biological and Pharmacological Activity

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Eglerisine, a Novel Sesquiterpenoid Tropolone from Dulacia egleri with Antiproliferative Effect against an Acute Myeloid Leukemia Lineage

Leice M. R. de Novais

¹Departamento de Química, Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil

,

Luiz F. Ferreira

¹Departamento de Química, Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil

,

Paulo T. de Sousa Jr.

¹Departamento de Química, Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil

,

Tereza A. N. Ribeiro

¹Departamento de Química, Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil

,

Marcos J. Jacinto

¹Departamento de Química, Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil

,

Carlos H. C. dos Santos

²Departamento de Química, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil

,

Mário G. de Carvalho

²Departamento de Química, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil

,

Heron F. V. Torquato

³Departamento de Bioquímica, Universidade Federal de São Paulo, São Paulo, SP, Brazil

⁴Faculdade de Farmácia, Braz Cubas Educação, Mogi das Cruzes, SP, Brazil

,

Edgar J. Paredes-Gamero

³Departamento de Bioquímica, Universidade Federal de São Paulo, São Paulo, SP, Brazil

⁵Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição (FACFAN), Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil

,

Virgínia C. P. Silva

¹Departamento de Química, Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil

› Author Affiliations

Abstract

Chemical investigation of the stems of Dulacia egleri resulted in the isolation of eglerisine (1), a compound with a rare sesquiterpenoid tropolone skeleton. Its structure was determined by analysis of spectrometric and spectroscopic data, including HRESIMS, 1D, and 2D NMR. The antiproliferative effects of eglerisine were tested in human leukemia lineages. In the Kasumi-1 lineage, an acute myeloid leukemia cell line, eglerisine reduced cell metabolism, as determined by the resazurin assay. Eglerisine did not induce cell death by either apoptotic or necrotic mechanisms. However, a reduction of the absolute number of cells was observed. Eglerisine induced cell cycle arrest after 72 h of treatment by phosphorylation of H2AX histone, reducing the S phase and increasing the G2 phase of the cell cycle.

Key words

Dulacia egleri - Olacaceae - tropolone sesquiterpene - cell cycle arrest

Full Text

References

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