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Planta Med 2020; 86(01): 45-54
DOI: 10.1055/a-1023-7385
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Effects of Sargaquinoic Acid in Sargassum Serratifolium on Inducing Brown Adipocyte-like Phenotype in Mouse Adipocytes In Vitro

Misung Kwon‡
1   Department of Food Science and Nutrition, Pukyong National University, Busan, Republic of Korea
,
Bonggi Lee‡
2   Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM), Daegu, Republic of Korea
,
Sujin Lim
1   Department of Food Science and Nutrition, Pukyong National University, Busan, Republic of Korea
,
Hyeung-Rak Kim
1   Department of Food Science and Nutrition, Pukyong National University, Busan, Republic of Korea
› Author Affiliations Acknowledgements: This work was supported by the project “Development of nutraceuticals from Sargassum serratifolium” funded by the Ministry of Oceans and Fisheries, Republic of Korea [20150311].
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Publication History

received 07 August 2019
revised 01 October 2019

accepted 03 October 2019

Publication Date:
29 October 2019 (online)

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Abstract

A previous study showed that the meroterpenoid-rich fraction of an ethanolic extract of Sargassum serratifolium (MES) stimulated adipose tissue browning and inhibited diet-induced obesity and metabolic syndrome. Sargaquinoic acid (SQA) is a major component in MES. We investigated the effects of SQA on the differentiation of preadipocytes to the beige adipocytes. SQA was treated in 3T3-L1 adipocytes differentiated under a special condition that has been reported to induce the browning of adipocytes. SQA at 10 µM reduced lipid accumulation by approximately 23%. SQA at 2.5 – 10 µM induced the differentiation of white adipocytes to beige adipocytes partially by increasing the mitochondrial density and the expression of beige/brown adipocyte markers. In addition, SQA activated lipid catabolic pathways, evidenced by the increased expression levels of perilipin, carnitine palmitoyltransferase 1, and acyl-CoA synthetase long-chain family member 1. As a partial mechanism, biochemical and in silico analyses indicate that SQA activated AMP-activated protein kinase signaling in adipocytes.

Key words

Sargassaceae - Sargassum serratifolium - lipolysis - sargaquinoic acid - adipocytes
 

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