Bioactive Properties of the Aqueous Extracts of Endophytic Fungi Associated with Scots Pine (Pinus sylvestris) Roots

Jenni Tienaho; Maarit Karonen; Riina Muilu-Mäkelä; Janne Kaseva; Nuria de Pedro; Francisca Vicente; Olga Genilloud; Ulla Aapola; Hannu Uusitalo; Katriina Vuolteenaho; Robert Franzén; Kristiina Wähälä; Matti Karp; Ville Santala; Tytti Sarjala

doi:10.1055/a-1185-4437

Planta Medica, Inhaltsverzeichnis

Planta Med 2020; 86(13/14): 1009-1024
DOI: 10.1055/a-1185-4437

Biological and Pharmacological Activity

Original Papers

Bioactive Properties of the Aqueous Extracts of Endophytic Fungi Associated with Scots Pine (Pinus sylvestris) Roots

Jenni Tienaho

¹Faculty of Natural Sciences and Engineering, Tampere University, Tampere, Finland

²Natural Resources Institute Finland (Luke), Production Systems Unit, Biomass Characterization and Properties Group, Espoo, Finland

,

Maarit Karonen

³Natural Chemistry Research Group, Department of Chemistry, University of Turku, Turku, Finland

,

Riina Muilu-Mäkelä

²Natural Resources Institute Finland (Luke), Production Systems Unit, Biomass Characterization and Properties Group, Espoo, Finland

,

Janne Kaseva

⁴Natural Resources Institute Finland (Luke), Natural Resources Unit, Applied Statistical Methods Group, Jokioinen, Finland

,

Nuria de Pedro

⁵Fundación MEDINA, Avda. del Conocimiento, Granada, Spain

,

Francisca Vicente

⁵Fundación MEDINA, Avda. del Conocimiento, Granada, Spain

,

Olga Genilloud

⁵Fundación MEDINA, Avda. del Conocimiento, Granada, Spain

,

Ulla Aapola

⁶Department of Ophthalmology, Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland

,

Hannu Uusitalo

⁶Department of Ophthalmology, Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland

⁷Tays Eye Centre, Tampere University Hospital, Tampere, Finland

,

Katriina Vuolteenaho

⁸The Immunopharmacology Research Group, Faculty of Medicine and Health Technology, Tampere University and Tampere University Hospital, Tampere, Finland

,

Robert Franzén

⁹School of Chemical Engineering, Department of Chemistry and Materials Science, Aalto University, Espoo, Finland

,

Kristiina Wähälä

¹⁰Department of Biochemistry and Developmental Biology, University of Helsinki, Helsinki, Finland

¹¹Department of Chemistry, University of Helsinki, Helsinki, Finland

,

Matti Karp

¹Faculty of Natural Sciences and Engineering, Tampere University, Tampere, Finland

,

Ville Santala

¹Faculty of Natural Sciences and Engineering, Tampere University, Tampere, Finland

,

Tytti Sarjala

²Natural Resources Institute Finland (Luke), Production Systems Unit, Biomass Characterization and Properties Group, Espoo, Finland

› Institutsangaben

Abstract

Despite the continuing interest in various plant and natural products, only a small portion of the biologically active compounds from nature has been discovered and exploited. In this study, antioxidant and antibacterial properties of aqueous fractions of three endophytic fungi isolated from the roots of 8-year-old Scots pines (Pinus sylvestris) growing on a drained peatland were investigated. The endophytic fungi species were Acephala applanata, Phialocephala fortinii, and Humicolopsis cephalosporioides/Coniochaeta mutabilis. The bioactivities were examined using hydrogen peroxide scavenging and oxygen radical absorbance capacity tests as well as sensitive Escherichia coli-based biosensors, which produce a luminescent signal in the presence of substances with oxidative or genotoxic properties. In addition, cell models for Parkinsonʼs disease, age-related macular degeneration, and osteoarthritis were used to evaluate the potential for pharmaceutical applications. The aqueous extracts of fungi and 19 out of 42 fractions were found to be active in one or more of the tests used. However, no activity was found in the age-related macular degeneration and osteoarthritis cell model tests. Additionally, bioactivity data was connected with metabolites putatively annotated, and out of 330 metabolites, 177 were interesting in view of the bioactivities investigated. A majority of these were peptides and all three fungal species shared a highly similar metabolome. We propose that Scots pine endophytic fungi are a rich source of interesting metabolites, and synergistic effects may cause the bioactivities, as they were found to vary after the fractionation process.

Key words

Acephala applanate - antibacterial - antioxidant - Coniochaeta mutabilis - endophytic fungi - Humicolopsis cephalosporioides - Leotimycetidae - Phialocephala fortinii - Sordariomycetidae

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Referenzen

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