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Planta Med 2022; 88(08): 628-638
DOI: 10.1055/a-1528-1760
Biological and Pharmacological Activity
Original Papers

Suffrutines A and B Inhibit the Expression of Inflammatory Mediators in LPS-Induced RAW264.7 Cells by Suppressing the NF-κB Signaling Pathway

Chun Chen
1   School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, China
,
Ze-Feng Zhu
1   School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, China
2   Department of Pharmacy, the Fifth Affiliated Hospital of Jinan University, Heyuan, Guangdong, China
,
Wen-Xing Nie
1   School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, China
,
Yong Zou
1   School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, China
3   Guangdong Provincial Key Laboratory of Chiral Molecule and Drug Discovery, Guangzhou, Guangdong, China
› Author AffiliationsSupported by: The National Natural Science Foundation of China 21272280
Supported by: The National Key Research and Development Project of China 2016YFA0602900
Supported by: The Key Project of Guangdong Natural Science Foundation 2016A030311033
Supported by: STPGC 201604020125
Supported by: Basic and Applied Basic Research Foundation of Guangdong Province Appl: 011249461088
Supported by: Guangdong Provincial Key Laboratory of Construction Foundation 2019B030301005
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Abstract

Flueggea suffruticosa is a traditional Chinese medicine that has been commonly used for the treatment of inflammatory ailments, including rheumatism and lumbago. Suffrutines A and suffrutines B are a pair of novel E,E and Z,E isomeric indolizidine alkaloids isolated from the roots of F. suffruticosa. However, their anti-inflammatory activity has not been reported thus far. The aim of this study was to investigate the inhibitory effect of inflammatory mediators and possible mechanisms of suffrutines A and B in lipopolysaccharide-induced RAW264.7 cells. Results showed that suffrutines A and B could remarkably inhibit the production of nitric oxide, prostaglandin E2, interleukin-6, inducible nitric oxide synthase, and cyclooxygenase-2 in lipopolysaccharide-induced RAW264.7 cells. Further evaluation demonstrated that compared with suffrutines A, suffrutines B could more significantly inhibit the phosphorylation of IKKα/β, the degradation of IκBα, and the nuclear translocation of the p65 and p52 subunits in the canonical and non-canonical nuclear factor-κB pathways. Therefore, suffrutines B exhibited more potent inhibitory activity on inflammatory mediators than suffrutines A.

Key words

Euphorbiaceae - Flueggea suffruticosa - inflammatory mediators - NF-κB signaling pathway - RAW264.7 - suffrutines A - suffrutines B

Supporting Information

    A series of NMR spectra and single-crystal X-ray crystallography of SA and SB are available as Supporting Information.

  • Supporting Information


Publication History

Received: 10 February 2021

Accepted after revision: 12 June 2021

Article published online:
22 July 2021

© 2021. Thieme. All rights reserved.

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