Cytochalasan Alkaloids as TRAIL Sensitizers from an Endophytic Fungus
						Chaetomium sp.

Ying Gao; Ruihua Mao; Ye Liu; Ming Zhou; Hanli Ruan

doi:10.1055/a-1728-4963

Planta Medica, Table of Contents

Planta Med 2022; 88(14): 1293-1298
DOI: 10.1055/a-1728-4963

Biological and Pharmacological Activity

Original Papers

Cytochalasan Alkaloids as TRAIL Sensitizers from an Endophytic Fungus Chaetomium sp.

Ying Gao

¹School of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology, Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, Wuhan, Peopleʼs Republic of China

,

Ruihua Mao

¹School of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology, Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, Wuhan, Peopleʼs Republic of China

²Department of Pharmacy, Tongji Hospital Affiliated Huazhong University of Science and Technology, Wuhan, Peopleʼs Republic of China

,

Ye Liu

³Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Peopleʼs Republic of China

,

Ming Zhou

³Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Peopleʼs Republic of China

,

Hanli Ruan

¹School of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology, Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, Wuhan, Peopleʼs Republic of China

› Author Affiliations

Abstract

Two new cytochalasans with a rare 6/6/5/5/7 pentacyclic ring system, named chaetoconvosins C−D (1−2), together with two known congeners (3−4), were isolated from the fermentation of an endophytic fungus, Chaetomium sp. SG-01, harbored in the fibrous roots of Schisandra glaucescens Diels. Their structures including the absolute configuration were elucidated by extensive spectroscopic (HRESIMS, NMR, and ECD) and X-ray crystallographic analyses. The TRAIL-resistance-overcoming activity of 1–4 in a TRAIL-resistant HT29 colorectal cancer cell line was evaluated, which revealed that co-treatment of 1–4 at 50 µM with TRAIL (150 ng/mL) reduced the HT29 cell viability by 19.0%, 24.1%, 17.9%, and 15.5%, respectively, compared to treatment with 1–4 alone.

Key words

Chaetomiaceae - Chaetomium sp. - cytochalasan alkaloids - cytotoxicity - TRAIL

Full Text

References

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