Subscribe to RSS
![](/products/assets/desktop/img/oa-logo.png)
DOI: 10.1055/a-1996-8940
Organocatalytic Synthesis of γ-Amino Acid Precursors via Masked Acetaldehyde under Micellar Catalysis
Abstract
The development of micellar catalysis offers a sustainable alternative to organic solvents, and represents an environmental milestone in organic synthesis. Here, the first Michael addition of masked acetaldehyde under neutral, cationic and anionic micellar catalysis is reported, affording the products in high yields and enantiomeric excess, despite the use of water as solvent.
#
Key words
micellar catalysis - aminocatalysis - asymmetric synthesis - γ-amino acids - green chemistryMicellar catalysis represents an innovative approach to perform catalysis in aqueous solvents that would otherwise be less efficient.[1] In fact, reducing the use of toxic or harmful organic solvents is one of the main goals of the scientific community.[2] However, since this often clashes with the efficiency of the reaction, nonconventional reaction media have been developed, such as ionic liquids,[3] deep eutectic[4] or fluorous solvents,[5] and supercritical fluids.[6]
In the framework of a long-term project aimed at developing methods exploiting less harmful reagents, we previously reported the use of masked acetaldehyde to effect the aminocatalytic enantioselective synthesis of intermediates to γ-amino acids. The strategy relied on the in-situ deprotection of masked acetaldehyde via synergistic use of chiral secondary amines and a Brønsted acid co-catalyst (Scheme [1]), and was inspired by the breakthrough contributions of List[7] and Hayashi,[8] among others.[9] However, chloroform was the best solvent in the first report to successfully deliver the products of interest in high yields and ee.[10]
![](https://www.thieme-connect.de/media/10.1055-s-00032269/202301/thumbnails/i_d0064_s1_10-1055_a-1996-8940.gif)
![Zoom Image](/products/assets/desktop/css/img/icon-figure-zoom.png)
With the objective of moving away from chlorinated solvents, we explored the chemical space via Design of Experiments, and developed a protocol in water as a solvent,[11] albeit using an ad-hoc designed catalyst that would present longer aliphatic chains.[12]
Therefore, we planned to use water as reaction medium alongside commercially available, privileged Jørgensen–Hayashi chiral amine in the asymmetric Michael addition of masked acetaldehyde to nitroalkenes under micellar catalysis.[13] We tackled the synthetic problem both from the synthetic and supramolecular points of view. In particular, it was decided to test the efficacy of anionic, cationic and neutral aggregates formed by sodium dodecyl sulfate (SDS), cetyltrimethylammonium bromide (CTAB) and Triton X-100 (TR-X100), respectively. We started our study by investigating the behavior of the reaction components in micelles via NOESY NMR analysis employing the cost-effective surfactants noted above (Table [1]).[14]
Surfactant [S] |
![]() |
![]() |
![]() |
1a |
2 |
3·H |
|
CTAB [150 mM] |
X |
V |
V |
SDS [450 mM] |
V |
V |
X |
TR-X100 [50 mM] |
V |
V |
V |
a X indicates no correlation, V indicates correlation as evidenced via NOESY.
The concentration of the samples for these qualitative measurements was chosen on the basis of the different critical micellar concentration of the surfactants and the complete solubilisation of the catalyst and reagents. Indeed, CTAB showed NOESY correlation both with the acetaldehyde dimethyl acetal 2 and the protonated Hayashi–Jørgensen catalyst 3·H, whereas there was no correlation with nitrostyrene 1a. On the other hand, SDS showed NOESY correlation with 1a and 2 but, based on the correlations, the protonated chiral amine 3·H seems to be unable to enter the micelles. Gratifyingly, neutral TR-X100 showed correlation with all the reaction components, suggesting a good potential for the synthesis of γ-amino acids precursors via masked acetaldehyde under micellar catalysis.
1H NMR spectra of water-soluble 2 and slightly soluble 3·H in pure D2O and in 50 mM solution of TR-X100 show that, in the presence of the micellar system, the signals are more resolved. This result indicates that both compounds are included in micelles as monomers, whereas in water they tend to aggregate. In particular, the NOESY analysis showed correlation between 1a with the polyethylene glycol (PEG) moieties and the aliphatic core of the micelle. A similar behavior was observed for the protonated Hayashi–Jørgensen catalyst 3·H. Finally, the protected acetaldehyde 2 showed correlation with the aromatic part of TR-X100, although this observation does not exclude a fast diffusion in the whole micelle.
Driven by these promising results, we established the feasibility of our protocol by examining the reaction of trans-β-nitrostyrene 1a in the presence of 2 equivalents of acetaldehyde 2-methyl acetal 2 activated by catalytic amounts of Amberlyst-15 and Jørgensen–Hayashi chiral amine in water at room temperature under micellar catalysis (Table [2]).
a Reaction conditions: 2 (0.4 mmol, 2 equiv), chiral amine (10 mol%) and 1 (0.2 mmol, 1 equiv) were added to a solution of surfactant and water (1 mL), then Amberlyst-15 (4 mg, 10 mol%), 25 °C, 20 h.
b Conversions were determined by 1H NMR analysis.
c Determined by chiral HPLC analysis after conversion of the aldehyde into the corresponding alcohol by reduction with NaBH4.
Screening different catalyst loading showed that by using 10 mol% of 3 in TR-X100 at 50 mM concentration we obtained full conversion of nitroalkene 1 into product 4 with excellent ee (Table [2], entries 1–4). On the other hand, Jørgensen–Hayashi 5 did not show the same reactivity (entry 5). Both reducing the PEG portion of the triton as well as lowering the concentration of the surfactant were detrimental for the reaction (entries 6 and 7). Tocopherol-based TPGS-750-M, a neutral surfactant commonly used in micellar catalysis, was not optimal for this reaction (entry 8). This evidence indicates that even subtle variations in the surfactant structure can affect the properties of the aggregates in terms of water penetration and/or packing of alkyl chains in the micellar core with a consequent effect on the catalytic performance.
CTAB and SDS suppressed reactivity, probably because not all reagents and catalyst are embedded in micelles (Table [2], entries 9 and 10). In the case of SDS micelles, the formation of an amine-sulfate ion pair between 3·H and SDS is possible, as reported for similar systems.[1g] This ion pair formation sequesters the catalyst, hampering the carbonyl activation. The reaction was tested at lower CTAB and SDS concentration, with a slight improvement in the reactivity being observed: in this case, probably, some of the components do not interact with micelles and thus undergo in an “on water” reaction.[14] In fact, when no surfactant was used, the reaction was sluggish, but not completely inhibited (entry 11). With the optimised conditions in hand (10 mol% 3 together with 10 mol% Amberlyst-15 in a 50 mM solution of TR-X100 in water; entry 4), we evaluated the generality of the reaction. We tested various substituted trans-β-nitrostyrenes 1a–g with 10 mol% of Jørgensen–Hayashi and Amberlyst-15 as catalysts in a 50 mM solution of TR-X100 (Scheme [2]). The reaction proceeded smoothly with both electron-rich and electron-poor substrates, affording the desired products in good yield and excellent enantiomeric excess. Employing trans-β-nitrostyrene 1c–f resulted in poor conversion under the standard conditions. By increasing the number of equivalents of masked acetaldehyde and the reaction time, products 4c–f were obtained in 55–96% yield and 92–94% ee. Notably, both chemical yields and the stereoselectivities of the desired products are comparable to those obtained in chlorinated solvents,[10] and clearly superior to those of our previous report in which water was employed as reaction medium.[11] Unfortunately, this protocol is limited to aromatic nitroalkenes; aliphatic substrates afforded low yields due to the formation of several by-products.[15] This limitation was also observed in our previous report, supporting the speculation that the solvent may play an important role. Further studies are ongoing in our laboratories to elucidate this aspect and overcome this limitation.
![](https://www.thieme-connect.de/media/10.1055-s-00032269/202301/thumbnails/i_d0064_s2_10-1055_a-1996-8940.gif)
![Zoom Image](/products/assets/desktop/css/img/icon-figure-zoom.png)
In summary, we reported the first Michael addition of masked acetaldehyde in water under cost-effective micellar catalysis.[16] The use of Triton X-100 proved to be crucial for including the reaction partners in the hydrophobic core of the micelle more than analogous neutral surfactants. On the other hand, anionic or cationic micelles were unable to include all the components of the reaction. Our results demonstrate that surfactant charge plays a pivotal role in determining the efficacy of the overall catalytic system. The optimized system proved to be successful for a library of aromatic nitroalkenes, showing reactivities and stereoselectivities comparable those obtained employing organic solvents.
#
Conflict of Interest
The authors declare no conflict of interest.
Supporting Information
- Supporting information for this article is available online at https://doi.org/10.1055/a-1996-8940.
- Supporting Information
-
References and Notes
- 1a Strukul G, Fabris F, Scarso A. In Supramolecular Catalysis . van Leeuwen PW, Raynal M. Wiley-VCH; Weinheim: 2022: 451
- 1b Cortes-Clerget M, Kincaid JR. A, Akporji N, Lipshutz BH. In Supramolecular Catalysis . van Leeuwen PW, Raynal M. Wiley-VCH; Weinheim: 2022: 467
- 1c Scarso A, Strukul G. In Green Synthetic Processes and Procedures . RSC; London: 2019: 268
- 1d Scarso A. In Encyclopedia of Inorganic and Bioinorganic Chemistry . Scott RA. Wiley-VCH; Weinheim: 2016: 1
- 1e Borrego E, Caballero A, Pérez PJ. Organometallics 2022; 41: 3084 DOI: 10.1021/acs.organomet.2c00309.
- 1f Lorenzetto T, Frigatti D, Fabris F, Scarso A. Adv. Synth. Catal. 2022; 364: 1776
- 1g Shen T, Zhou S, Ruan J, Chen X, Liu X, Ge X, Qian C. Adv. Colloid Interface Sci. 2021; 287: 102299
- 1h La Sorella G, Strukul G, Scarso A. Green Chem. 2015; 17: 644
- 2 Zimmerman JB, Anastas PT, Erythropel HC, Leitner W. Science 2020; 367: 397
- 3 Lei Z, Chen B, Koo Y.-M, MacFarlane DR. Chem. Rev. 2017; 117: 6633
- 4 Hansen BB, Spittle S, Chen B, Poe D, Zhang Y, Klein JM, Horton A, Adhikari L, Zelovich T, Doherty BW, Gurkan B, Maginn EJ, Ragauskas A, Dadmun M, Zawodzinski TA, Baker GA, Tuckerman ME, Savinell RF, Sangoro JR. Chem. Rev. 2021; 121: 1232
- 5 Zhang W. Green Chem. 2009; 11: 911
- 6 Noyori R. Chem. Rev. 1999; 99: 353
- 7 García-García P, Ladépêche A, Halder R, List B. Angew. Chem. Int. Ed. 2008; 47: 4719
- 8a Hayashi Y, Itoh T, Ohkubo M, Ishikawa H. Angew. Chem. Int. Ed. 2008; 47: 4722
- 8b Ishikawa H, Honma M, Hayashi Y. Angew. Chem. Int. Ed. 2011; 50: 2824
- 9a Meng X.-L, Liu T, Sun Z.-W, Wang J.-C, Peng F.-Z, Shao Z.-H. Org. Lett. 2014; 16: 3044
- 9b Feu KS, de la Torre AF, Silva S, de Moraes MA. F. Jr, Corrêa AG, Paixão MW. Green Chem. 2014; 16: 3169
- 9c Ramachary DB, Reddy PS, Prasad MS. Eur. J. Org. Chem. 2014; 3076
- 9d Fan X, Rodríguez-Escrich C, Sayalero S, Pericàs MA. Chem. Eur. J. 2013; 19: 10814
- 9e Geertsema EM, Miao Y, Tepper PG, Dehaan P, Zandvoort E, Poelarends GJ. Chem. Eur. J. 2013; 19: 14407
- 9f Qiao Y, He J, Ni B, Headley AD. Adv. Synth. Catal. 2012; 354: 2849
- 9g Alza E, Pericàs MA. Adv. Synth. Catal. 2009; 351: 3051
- 10 Giorgianni G, Nori V, Baschieri A, Palombi L, Carlone A. Catalysts 2020; 11: 10
- 11a Nori V, Sinibaldi A, Giorgianni G, Pesciaioli F, Di Donato F, Cocco E, Biancolillo A, Landa A, Carlone A. Chem. Eur. J. 2022; 28: e202104524
- 11b Nori V, Sinibaldi A, Pesciaioli F, Carlone A. Synthesis 2022; 54: 4246
- 12 Palomo C, Landa A, Mielgo A, Oiarbide M, Puente Á, Vera S. Angew. Chem. Int. Ed. 2007; 46: 8431
- 13a Delgado JA. C, Vicente FE. M, de la Torre AF, Fernandes VA, Corrêa AG, Paixão MW. New J. Chem. 2021; 45: 14050
- 13b Soares BM, Aguilar AM, Silva ER, Coutinho-Neto MD, Hamley IW, Reza M, Ruokolainen J, Alves WA. Phys. Chem. Chem. Phys. 2017; 19: 1181
- 13c Neumann LN, Baker MB, Leenders CM. A, Voets IK, Lafleur RP. M, Palmans AR. A, Meijer EW. Org. Biomol. Chem. 2015; 13: 7711
- 13d Lipshutz BH, Ghorai S. Org. Lett. 2012; 14: 422
- 14 García-García P, Moreno JM, Díaz U, Bruix M, Corma A. Nat. Commun. 2016; 7: 10835
- 15 For further information see the Supporting Information.
- 16 Asymmetric Michael Addition of Acetaldehyde Dimethyl Acetal Performed in Micelles; General Procedure: Acetaldehyde dimethyl acetal 2 (2–4 equiv) was added to a 4 mL scintillation vial equipped with a magnetic bar, containing a solution 50 mM of Triton X-100 (31 mg, 0.05 mmol, 0.25 equiv) in water (1 mL), (S)-diphenyltrimethylsiloxymethylpyrrolidine 3 (6.5 mg, 0.02 mmol, 0.1 equiv), nitroalkene 1a–e (0.2 mmol, 1 equiv), Amberlyst-15 (4.5 mg, 10 mol%), under stirring, and the reaction mixture was stirred at room temperature for the required time. Brine (1 mL) was then added and the aqueous mixture was extracted with THF (1 mL), and CHCl3 (3 × 1 mL). The combined organic layers were dried over MgSO4 and concentrated in vacuo after filtration. The crude mixture was purified by flash chromatography on SiO2 using a mixture of petroleum ether/ethyl acetate 9:1 v/v to give the desired product. NMR spectra of previously reported compounds were in agreement with those of the authentic samples and/or available literature data. Information can be used for the characterization data of other products. (S)-4-Nitro-3-phenylbutanal (4a): Synthesized in accordance with the general procedure for asymmetric Michael addition of acetaldehyde dimethyl acetal performed in micelles, using acetaldehyde dimethyl acetal 2 (42.2 μL, 0.4 mmol) and trans-β-nitrostyrene 1 (30 mg, 0.2 mmol). The reaction mixture was stirred at room temperature for 20 h. The desired product (yield: 35 mg, 0.18 mmol, 90%) was obtained as a pale-yellow oil. 1H NMR (400 MHz, CDCl3, 303 K): δ = 9.72 (s, 1 H), 7.37 (t, J = 7.3 Hz, 2 H), 7.31 (d, J = 6.1 Hz, 1 H), 7.28–7.23 (m, 2 H), 4.68 (dd, J = 12.5, 7.2 Hz, 1 H), 4.62 (dd, J = 12.5, 7.5 Hz, 1 H), 4.10 (m, 1 H), 2.97 (d, J = 7.3 Hz, 2 H). 13C NMR (101 MHz, CDCl3): δ = 198.7, 138.2, 129.2, 128.1, 127.4, 79.4, 46.4, 38.0. HPLC (Lux 3μm-cellulose 1, hexane/i-propanol 90:10, flow: 0.5 mL/min, λ = 210 nm): tR = 19.6 (minor), 24.7 (major) min. [α]D 25 = –18.13 (c = 0.0016 g/mL, CHCl3). All analytical data were in good accordance with reported data.10
For selected examples of organocatalytic reactions under micellar catalysis, where the catalyst is covalently bound with the surfactant, see:
Corresponding Authors
Publication History
Received: 15 November 2022
Accepted after revision: 12 December 2022
Accepted Manuscript online:
12 December 2022
Article published online:
30 January 2023
© 2022. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by/4.0/)
Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany
-
References and Notes
- 1a Strukul G, Fabris F, Scarso A. In Supramolecular Catalysis . van Leeuwen PW, Raynal M. Wiley-VCH; Weinheim: 2022: 451
- 1b Cortes-Clerget M, Kincaid JR. A, Akporji N, Lipshutz BH. In Supramolecular Catalysis . van Leeuwen PW, Raynal M. Wiley-VCH; Weinheim: 2022: 467
- 1c Scarso A, Strukul G. In Green Synthetic Processes and Procedures . RSC; London: 2019: 268
- 1d Scarso A. In Encyclopedia of Inorganic and Bioinorganic Chemistry . Scott RA. Wiley-VCH; Weinheim: 2016: 1
- 1e Borrego E, Caballero A, Pérez PJ. Organometallics 2022; 41: 3084 DOI: 10.1021/acs.organomet.2c00309.
- 1f Lorenzetto T, Frigatti D, Fabris F, Scarso A. Adv. Synth. Catal. 2022; 364: 1776
- 1g Shen T, Zhou S, Ruan J, Chen X, Liu X, Ge X, Qian C. Adv. Colloid Interface Sci. 2021; 287: 102299
- 1h La Sorella G, Strukul G, Scarso A. Green Chem. 2015; 17: 644
- 2 Zimmerman JB, Anastas PT, Erythropel HC, Leitner W. Science 2020; 367: 397
- 3 Lei Z, Chen B, Koo Y.-M, MacFarlane DR. Chem. Rev. 2017; 117: 6633
- 4 Hansen BB, Spittle S, Chen B, Poe D, Zhang Y, Klein JM, Horton A, Adhikari L, Zelovich T, Doherty BW, Gurkan B, Maginn EJ, Ragauskas A, Dadmun M, Zawodzinski TA, Baker GA, Tuckerman ME, Savinell RF, Sangoro JR. Chem. Rev. 2021; 121: 1232
- 5 Zhang W. Green Chem. 2009; 11: 911
- 6 Noyori R. Chem. Rev. 1999; 99: 353
- 7 García-García P, Ladépêche A, Halder R, List B. Angew. Chem. Int. Ed. 2008; 47: 4719
- 8a Hayashi Y, Itoh T, Ohkubo M, Ishikawa H. Angew. Chem. Int. Ed. 2008; 47: 4722
- 8b Ishikawa H, Honma M, Hayashi Y. Angew. Chem. Int. Ed. 2011; 50: 2824
- 9a Meng X.-L, Liu T, Sun Z.-W, Wang J.-C, Peng F.-Z, Shao Z.-H. Org. Lett. 2014; 16: 3044
- 9b Feu KS, de la Torre AF, Silva S, de Moraes MA. F. Jr, Corrêa AG, Paixão MW. Green Chem. 2014; 16: 3169
- 9c Ramachary DB, Reddy PS, Prasad MS. Eur. J. Org. Chem. 2014; 3076
- 9d Fan X, Rodríguez-Escrich C, Sayalero S, Pericàs MA. Chem. Eur. J. 2013; 19: 10814
- 9e Geertsema EM, Miao Y, Tepper PG, Dehaan P, Zandvoort E, Poelarends GJ. Chem. Eur. J. 2013; 19: 14407
- 9f Qiao Y, He J, Ni B, Headley AD. Adv. Synth. Catal. 2012; 354: 2849
- 9g Alza E, Pericàs MA. Adv. Synth. Catal. 2009; 351: 3051
- 10 Giorgianni G, Nori V, Baschieri A, Palombi L, Carlone A. Catalysts 2020; 11: 10
- 11a Nori V, Sinibaldi A, Giorgianni G, Pesciaioli F, Di Donato F, Cocco E, Biancolillo A, Landa A, Carlone A. Chem. Eur. J. 2022; 28: e202104524
- 11b Nori V, Sinibaldi A, Pesciaioli F, Carlone A. Synthesis 2022; 54: 4246
- 12 Palomo C, Landa A, Mielgo A, Oiarbide M, Puente Á, Vera S. Angew. Chem. Int. Ed. 2007; 46: 8431
- 13a Delgado JA. C, Vicente FE. M, de la Torre AF, Fernandes VA, Corrêa AG, Paixão MW. New J. Chem. 2021; 45: 14050
- 13b Soares BM, Aguilar AM, Silva ER, Coutinho-Neto MD, Hamley IW, Reza M, Ruokolainen J, Alves WA. Phys. Chem. Chem. Phys. 2017; 19: 1181
- 13c Neumann LN, Baker MB, Leenders CM. A, Voets IK, Lafleur RP. M, Palmans AR. A, Meijer EW. Org. Biomol. Chem. 2015; 13: 7711
- 13d Lipshutz BH, Ghorai S. Org. Lett. 2012; 14: 422
- 14 García-García P, Moreno JM, Díaz U, Bruix M, Corma A. Nat. Commun. 2016; 7: 10835
- 15 For further information see the Supporting Information.
- 16 Asymmetric Michael Addition of Acetaldehyde Dimethyl Acetal Performed in Micelles; General Procedure: Acetaldehyde dimethyl acetal 2 (2–4 equiv) was added to a 4 mL scintillation vial equipped with a magnetic bar, containing a solution 50 mM of Triton X-100 (31 mg, 0.05 mmol, 0.25 equiv) in water (1 mL), (S)-diphenyltrimethylsiloxymethylpyrrolidine 3 (6.5 mg, 0.02 mmol, 0.1 equiv), nitroalkene 1a–e (0.2 mmol, 1 equiv), Amberlyst-15 (4.5 mg, 10 mol%), under stirring, and the reaction mixture was stirred at room temperature for the required time. Brine (1 mL) was then added and the aqueous mixture was extracted with THF (1 mL), and CHCl3 (3 × 1 mL). The combined organic layers were dried over MgSO4 and concentrated in vacuo after filtration. The crude mixture was purified by flash chromatography on SiO2 using a mixture of petroleum ether/ethyl acetate 9:1 v/v to give the desired product. NMR spectra of previously reported compounds were in agreement with those of the authentic samples and/or available literature data. Information can be used for the characterization data of other products. (S)-4-Nitro-3-phenylbutanal (4a): Synthesized in accordance with the general procedure for asymmetric Michael addition of acetaldehyde dimethyl acetal performed in micelles, using acetaldehyde dimethyl acetal 2 (42.2 μL, 0.4 mmol) and trans-β-nitrostyrene 1 (30 mg, 0.2 mmol). The reaction mixture was stirred at room temperature for 20 h. The desired product (yield: 35 mg, 0.18 mmol, 90%) was obtained as a pale-yellow oil. 1H NMR (400 MHz, CDCl3, 303 K): δ = 9.72 (s, 1 H), 7.37 (t, J = 7.3 Hz, 2 H), 7.31 (d, J = 6.1 Hz, 1 H), 7.28–7.23 (m, 2 H), 4.68 (dd, J = 12.5, 7.2 Hz, 1 H), 4.62 (dd, J = 12.5, 7.5 Hz, 1 H), 4.10 (m, 1 H), 2.97 (d, J = 7.3 Hz, 2 H). 13C NMR (101 MHz, CDCl3): δ = 198.7, 138.2, 129.2, 128.1, 127.4, 79.4, 46.4, 38.0. HPLC (Lux 3μm-cellulose 1, hexane/i-propanol 90:10, flow: 0.5 mL/min, λ = 210 nm): tR = 19.6 (minor), 24.7 (major) min. [α]D 25 = –18.13 (c = 0.0016 g/mL, CHCl3). All analytical data were in good accordance with reported data.10
For selected examples of organocatalytic reactions under micellar catalysis, where the catalyst is covalently bound with the surfactant, see:
![](https://www.thieme-connect.de/media/10.1055-s-00032269/202301/thumbnails/i_d0064_s1_10-1055_a-1996-8940.gif)
![Zoom Image](/products/assets/desktop/css/img/icon-figure-zoom.png)
![](https://www.thieme-connect.de/media/10.1055-s-00032269/202301/i_d0064_t1-1_10-1055_a-1996-8940.gif)
![](https://www.thieme-connect.de/media/10.1055-s-00032269/202301/i_d0064_t1-2_10-1055_a-1996-8940.gif)
![](https://www.thieme-connect.de/media/10.1055-s-00032269/202301/i_d0064_t1-3_10-1055_a-1996-8940.gif)
![](https://www.thieme-connect.de/media/10.1055-s-00032269/202301/i_d0064_t2_10-1055_a-1996-8940.gif)
![](https://www.thieme-connect.de/media/10.1055-s-00032269/202301/thumbnails/i_d0064_s2_10-1055_a-1996-8940.gif)
![Zoom Image](/products/assets/desktop/css/img/icon-figure-zoom.png)