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DOI: 10.1055/a-2072-2177
Phytochemical Analysis of Nothapodytes tomentosa and Distribution and Content of Camptothecin and its Analogues in Four Plants
This research was supported by the National Key R&D Program of China (2018YFA0900600), the National Natural Science Foundation of China (32000239 to J.-P. H.), the China Postdoctoral Science Foundation (2022M710497 to L. W.), and the Xinglin Scholar Research Promotion Project of Chengdu University of TCM (BSH2021013 to L. W.).![](https://www.thieme-connect.de/media/plantamedica/202313/lookinside/thumbnails/10-1055-a-2072-2177_pmg0837-1.jpg)
Abstract
Camptothecin (CPT) and its derivatives have attracted worldwide attention because of their notable anticancer activity. However, the growing demand for CPT in the global pharmaceutical industry has caused a severe shortage of CPT-producing plant resources. In this study, phytochemical analysis of Nothapodytes tomentosa results in the isolation and identification of CPT (13) and 16 analogues (1 – 12, 14 – 17), including a new (1) and five known (9, 10, 12, 15, and 17) CPT analogues with an open E-ring. In view of the potential anticancer activity of CPT analogues with an open E-ring, the fragmentation pathways and mass spectra profiles of these six CPT analogues (1, 9, 10, 12, 15, and 17) are investigated, providing a reference for the rapid detection of these compounds in other plants. Furthermore, based on the fragmentation patterns of CPT (13) and known analogues (2 – 8, 11, 14, 16, 18 – 26), the distribution and content of these compounds in different tissues of N. tomentosa, N. nimmoniana, Camptotheca acuminata, and Ophiorrhiza japonica are further studied. Our findings not only provide an alternative plant resource for further expanding the development and utilization of CPT and its analogues, but also lay a foundation for improving the utilization of known CPT-producing plant resources.
Key words
Nothapodytes tomentosa - Nothapodytes nimmoniana - Camptotheca acuminata - Ophiorrhiza japonica - Icacinaceae - camptothecin derivativesSupporting Information
- Supporting Information
Methods for ECD calculations, methods for optimization of extraction conditions, methods for qualitative and quantitative analysis of CPT and its analogues in four plants by UHPLC-Q-ESI-TOF-MS/MS and HPLC-Q-ESI-TOF-MS, NMR data of compounds 2 – 17, spectra of compound 1, ECD spectra of compounds 1, 9, and 12, structure of CPT and its analogues analyzed in this study, data on distribution of CPT and its analogues in different tissues of four plants, MS/MS spectra of compounds 1, 9, 12, 10, 15, and 17, proposed MS/MS fragmentation pathway of compounds 1, 9, and 12, data obtained by the single factor experiments and RSM, important thermodynamic parameters (a. u.) and Boltzmann distributions of the optimized compound 1, and optimized Z-matrixes of compound 1 are available as Supporting Information.
Publication History
Received: 15 December 2022
Accepted after revision: 11 April 2023
Accepted Manuscript online:
12 April 2023
Article published online:
28 July 2023
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