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DOI: 10.1055/a-2093-3528
CD44 and EGFR Dual-Targeted Antibody-Recruiting Complex Based on Hyaluronic Acid Grafted with β-Cyclodextrin and Multivalent Rhamnose for Cancer Immunotherapy
This work was supported by the National Natural Science Foundation of China (22177040, 32000904), the Natural Science Foundation of Jiangsu Province (BK20200601), China Postdoctoral Science Foundation (2018M632227 and 2021M691293). The project was partly funded by the 111 Project (No. 111-2-06).

Abstract
A new generation of multivalent antibody-recruiting molecules (ARMs) with dual-targeting tumor-binding termini (TBT), including hyaluronic acid targeting CD44 and nanobody 7D12 or peptide GE11 targeting EGFR, was constructed for cancer immunotherapy. The 7D12 or GE11 were assembled onto β-cyclodextrin-grafted hyaluronic acid (HACD) with multivalent rhamnose via host-guest interaction to form macromolecule complexes. The immunological studies proved that these complexes had dual-targetability on CD44 and EGFR and the rhamnose on HACD could recruit anti-Rha antibodies to mediate cytotoxicity against the targeted tumor cells. This bispecific ARM strategy provides a platform for cancer immunotherapy.
Key words
antibody-recruiting complex - cancer immunotherapy - dual-targeting - hyaluronic acid - host-guest interaction - 7D12 - GE11Supporting Information
- Supporting information for this article is available online at https://doi.org/10.1055/a-2093-3528.
- Supporting Information
Publication History
Received: 21 March 2023
Accepted after revision: 15 May 2023
Accepted Manuscript online:
15 May 2023
Article published online:
14 June 2023
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