Sargachromenol Attenuates Inflammatory Responses by Regulating NF-κB and Nrf2 Pathways in RAW 264.7 Cells and LPS-treated Mice

Eun-Ji Joung; Min-Kyeong Lee; Minsup Lee; Misung Gwon; Taisun Shin; Heeyeon Ryu; Hyeon Hak Jeong; Myeong-Jin Kim; Ji Yun Van; Jae-Il Kim; Jinkyung Choi; Won-Kyo Jung; Hyeung-Rak Kim; Bonggi Lee

doi:10.1055/a-2180-1338

Planta Medica, Table of Contents

Planta Med 2024; 90(01): 25-37
DOI: 10.1055/a-2180-1338

Biological and Pharmacological Activity

Original Papers

Sargachromenol Attenuates Inflammatory Responses by Regulating NF-κB and Nrf2 Pathways in RAW 264.7 Cells and LPS-treated Mice

Eun-Ji Joung‡

¹Department of Food Science and Nutrition, Pukyong National University, Busan, Republic of Korea

,

Min-Kyeong Lee‡

¹Department of Food Science and Nutrition, Pukyong National University, Busan, Republic of Korea

,

Minsup Lee

²Department of Molecular and Cellular Physiology, Louisiana State University Health Shreveport, Louisiana, United States

,

Misung Gwon

¹Department of Food Science and Nutrition, Pukyong National University, Busan, Republic of Korea

,

Taisun Shin

³Division of Food and Nutrition, Chonnam National University, Gwangju, Republic of Korea

,

Heeyeon Ryu

¹Department of Food Science and Nutrition, Pukyong National University, Busan, Republic of Korea

,

Hyeon Hak Jeong

⁴Department of Smart Green Technology Engineering, Pukyong National University, Busan, Republic of Korea

,

Myeong-Jin Kim

¹Department of Food Science and Nutrition, Pukyong National University, Busan, Republic of Korea

,

Ji Yun Van

⁴Department of Smart Green Technology Engineering, Pukyong National University, Busan, Republic of Korea

,

Jae-Il Kim

¹Department of Food Science and Nutrition, Pukyong National University, Busan, Republic of Korea

,

Jinkyung Choi

¹Department of Food Science and Nutrition, Pukyong National University, Busan, Republic of Korea

,

Won-Kyo Jung

⁵Division of Biomedical Engineering and Research Center for Marine Integrated Bionics Technology, Pukyong National University, Busan, Republic of Korea

,

Hyeung-Rak Kim

¹Department of Food Science and Nutrition, Pukyong National University, Busan, Republic of Korea

⁴Department of Smart Green Technology Engineering, Pukyong National University, Busan, Republic of Korea

,

Bonggi Lee

¹Department of Food Science and Nutrition, Pukyong National University, Busan, Republic of Korea

⁴Department of Smart Green Technology Engineering, Pukyong National University, Busan, Republic of Korea

› Author Affiliations

Abstract

This study aims to explore the anti-inflammatory mechanisms of sargachromenol in both RAW 264.7 cells and lipopolysaccharide (LPS)-treated mice, as previous reports have suggested that sargachromenol possesses anti-aging, anti-inflammatory, antioxidant, and neuroprotective properties. Although the precise mechanism behind its anti-inflammatory activity remains unclear, pretreatment with sargachromenol effectively reduced the production of nitric oxide, prostaglandin E₂, and interleukin (IL)-1β in LPS-stimulated RAW 264.7 cells by inhibiting cyclooxygenase-2. Moreover, sargachromenol inhibited the activation of nuclear factor-κB (NF-κB) by preventing the degradation of the inhibitor of κB-α (IκB-α) and inhibiting protein kinase B (Akt) phosphorylation in LPS-stimulated cells. We also found that sargachromenol induced the production of heme oxygenase-1 (HO-1) by activating the nuclear transcription factor erythroid-2-related factor 2 (Nrf2). In LPS-treated mice, oral administration of sargachromenol effectively reduced the levels of IL-1β, IL-6, and tumor necrosis factor-α (TNF-α) in the serum, suggesting its ability to suppress the production of inflammatory mediators by inhibiting the Akt/NF-κB pathway and upregulating the Nrf2/HO-1 pathway.

Key words

anti-inflammation - antioxidant - sargachromenol - Sargassum serratifolium - brown algae - sargassaceae - Nrf2

Full Text

References

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