Synthesis
DOI: 10.1055/a-2435-5962
short review

19F-Labeled NMR Probes for the Detection and Discrimination of Nitrogen-Containing Analytes

Guangxing Gu
a   Key Laboratory of Fluorine and Nitrogen Chemistry and Advanced Materials and Shanghai Hongkong Joint Laboratory in Chemical Synthesis, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, 200032, P. R. of China
,
Chenyang Wang
a   Key Laboratory of Fluorine and Nitrogen Chemistry and Advanced Materials and Shanghai Hongkong Joint Laboratory in Chemical Synthesis, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, 200032, P. R. of China
,
Yanchuan Zhao
a   Key Laboratory of Fluorine and Nitrogen Chemistry and Advanced Materials and Shanghai Hongkong Joint Laboratory in Chemical Synthesis, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, 200032, P. R. of China
b   Instrumental Analysis Center, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, 200032, P. R. of China
› Author Affiliations
This work was supported by the National Natural Science Foundation of China (22271305) and the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB0590000).


Abstract

The development of 19F-labeled NMR probes has become a pivotal tool in analytical chemistry. Recent advancements in probe design enable precise identification of nitrogen-containing analytes, significantly enhancing the analysis of these biologically important analytes in complex mixtures. This short review highlights recent progress with probes based on covalent derivatization and dynamic exchange strategies, which yield distinct 19F NMR signals for each nitrogen-containing analyte. These strategies facilitate separation-free multicomponent analysis and chiral discrimination. Discussions will cover design principles, scope, limitations, and strategies to enhance the sensitivity and resolving ability. By addressing current challenges, 19F-labeled NMR probes hold the potential to revolutionize the detection of biologically relevant molecules, catalyzing new discoveries in chemical and biochemical research.

1 Introduction

2 19F-Labeled Probes Based on Covalent Derivatization

2.1 Non-Chiral 19F-Labeled Probes Based on Covalent Derivatization

2.2 Chiral 19F-Labeled Probes Based on Covalent Derivatization

3 19F-Labeled Probes Based on Dynamic Ligand Exchange

3.1 Non-Chiral 19F-Labeled Probes Based on Dynamic Ligand Exchange

3.2 Chiral 19F-Labeled Probes Based on Dynamic Ligand Exchange

4 Conclusion and Outlook



Publication History

Received: 26 August 2024

Accepted after revision: 07 October 2024

Accepted Manuscript online:
07 October 2024

Article published online:
04 November 2024

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