Chemical-capture-mediated sensing has had a great impact on proteomic research. Toward
this end, we demonstrate the chemical trapping of BSA by the reactive formyl functionality
of a newly developed fluorescent nucleoside probe, formylphenothiazine-labeled-2′-deoxyuridine.
The probe is capable of trapping BSA via Schiff base formation leading to fluorescence
‘switch-on’ sensing with a large hypsochromic shift of ca. 100 nm. The α-amylase does
not show any significant change in fluorescence response, demonstrating the efficiency
of the probe in selective sensing of BSA. Docking studies suggest the preferential
interaction of the phenothiazinylcarbaldehyde-labeled dU with the residual amino acids
in site I of the BSA protein as compared to site II.
Key words
formylphenothiazine-labeled 2′-deoxyuridine - chemical capture - Schiff base - fluorimetric
sensing - bovine serum albumin - molecular docking
