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DOI: 10.1055/s-0028-1088334
© Georg Thieme Verlag KG Stuttgart · New York
Identification of Crude Drugs from Chinese Medicinal Plants of the Genus Bupleurum Using Ribosomal DNA ITS Sequences
Publication History
Received: September 20, 2007
Revised: August 25, 2008
Accepted: September 23, 2008
Publication Date:
18 November 2008 (online)
![](https://www.thieme-connect.de/media/plantamedica/200901/lookinside/thumbnails/10.1055-s-0028-1088334-1.jpg)
Abstract
To determine the variation of rDNA ITS sequences in medicinal plants of the genus Bupleurum and identify corresponding DNA molecular markers associated with medicinally important members, ITS regions were amplified, sequenced, and analyzed by DNAssist version 2.2. We found that the homologous alignment of ITS sequences between members of the genus Bupleurum and associated out-groups was lower than 75 %, while within-group alignment was greater than 87 %. The conclusion can be drawn that ITS sequences can be used as reliable molecular markers for the identification of Radix Bupleuri.
Key words
ITS sequence - Chaihu - Radix Bupleuri - Bupleurum - Umbelliferae (Apiaceae) - identification
References
- 1 Zhonghua Bencao editorial committee. Zhonghua Bencao. Shanghai; Shanghai Scientific & Technical Publishers 1998
- 2 Pan S L. Bupleurum species: scientific evaluation and clinical applications. Boca Raton; CRC Press 2006
- 3 Editorial committee of Flora of China. Flora of China, Volume 55. Beijing; Science Press 1979: 215-95
- 4 Pan S L, Shun Q C, Bo Q M, Bao X S. The coloured atlas of the medicinal plants from genus Bupleurum in China. Shanghai; Shanghai Science & Technology Literature Press 2002
- 5 Wang Y Z, Zhang Y Y. Determination of species of medical Bupleurum. . Chin Pharm J. 1994; 1 16
- 6 Pan S L. Investigation on resources of ”Chaihu” and identification of its commodities. Zhong Yao Cai. 1996; 5 231
- 7 Shao M L. Pharmacopoeia of the People’s Republic of China. Beijing; Chemical Industry Press 2005: 198
- 8 Mao R G, Lin D H, Wang Z H, Hong X K, Pan S L. Quantitative analysis of saikosaponin a, c, d in different species of Radix Bupleuri by HPLC. Chin Tradit Herb Drugs. 2002; 5 412
- 9 Wang J B, Zhang W J, Chen J K. Application of ITS sequences of nuclear rDNA in phylogenetic and evolutionary studies of angiosperms. Acta Phytotaxon Sin. 1999; 4 407
- 10 Xu H, Li X B, Wang Z T, Ding X Y, Xu L S, Zhou K Y. rDNA ITS sequencing of Herba Dendrobii (Huangchao). Acta Pharmacol Sin. 2001; 10 55-61
- 11 Liu J Q, Chen Z D, Liao Z X, Lu A M. A comparison of the ITS sequences of the Tibetan medicine ‘‘Zang Yin Chen’’-Swertia mussotti and its adultant species. Acta Pharmacol Sin. 2001; 1 68-71
- 12 Zeng M, Ma Y J, Zheng S Q, Xu J F, Di X H. Studies on ribosomal DNA sequence analyses of Radix Puerariae and its sibling species. Chin Pharm J. 2003; 3 13-5
- 13 Ding P, Fang Q. Ribosomal DNA-ITS sequence analysis and molecular identification of Morinda officinalis and its counterfeit species. Chin Tradit Herb Drugs. 2005; 6 114-7
- 14 Wang H, Wang Q. Analysis of rDNA ITS sequences of Radix et Rhizoma Salviae Miltiorrhizae and plants of Salvia L. Chin Tradit Herb Drugs. 2005; 9 105-9
- 15 Lau D TW, Shaw P C, Wang J, But P PH. Authentication of medicinal Dendrobium species by the internal transcribed spacer of ribosomal DNA. Planta Med. 2001; 67 456-60
- 16 Xu H, Wang Z T, Ding X Y, Zhou K Y, Xu L S. Differentiation of Dendrobium species used as ”Huangcao Shihu” by rDNA ITS sequence analysis. Planta Med. 2006; 72 89-92
- 17 Lee S KY, Li P T, Lau D TW, Yung P P, Kong R YC, Fong W F. Phylogeny of medicinal Phyllanthus species in China based on nuclear ITS and chloroplast atpB-rbcL sequences and multiplex PCR detection assay analysis. Planta Med. 2006; 72 721-6
- 18 Wu Y, Liu C S, Liu Y F, Yan Y N. ITS sequence identification of Radix Bupleuri. Zhongguo Zhong Yao Za Zhi. 2005; 10 732
- 19 Yang Z Y, Chao Z, Huo K K, Xie H, Tian Z P, Pan S L. ITS sequence analysis used for molecular identification of the Bupleurum species from northwestern China. Phytomedicine. 2007; 14 416-22
- 20 Neves S S, Watson M F. Phylogenetic relationships in Bupleurum (Apiaceae) based on nuclear ribosomal DNA ITS sequence data. Ann Bot (Lond). 2004; 4 379
- 21 Shu P, She M L. Pollen photographs and flora of Umbelliferae in China. Shanghai; Shanghai Scientific & Technical Publishers 2001: 68-75
Prof. Shengli Pan
School of Pharmacy
Fudan University
138 Yixueyuan Road
Shanghai 200032
People’s Republic of China
Phone: +86-21-5423-7454
Email: panshengli@online.sh.cn