Planta Med 2009; 75(3): 230-235
DOI: 10.1055/s-0028-1088377
Natural Products Chemistry
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Bakkenolides from Petasites tricholobus and Their Neuroprotective Effects Related to Antioxidant Activities

Yu-Liang Wang1 , 2 , Run-Ping Li3 , Mei-Li Guo1 , Ge Zhang1 , Na Zhang1 , Yan-Li Ma1
  • 1Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai, P.R. China
  • 2Plant Biotechnology Research Center, School of Agricultural and Biology, Shanghai Jiaotong University, Shanghai, P.R. China
  • 3Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, P.R. China
Further Information

Publication History

Received: May 4, 2008 Revised: October 16, 2008

Accepted: October 21, 2008

Publication Date:
15 December 2008 (online)

Abstract

Four novel bakkenolides – bakkenolide-Ia (1), bakkenolide-IIa (2), bakkenolide-IIIa (3) and bakkenolide-IVa (4) – were isolated from the extract of the rhizome of Petasites tricholobus. The structures were characterized by using NMR (1H, 13C, 1H–1H COSY, HMQC, HMBC, and NOESY) and mass spectrometry. The neuroprotective activity of the compounds 1 – 4 was assayed with primary cultured neurons exposed to oxygen-glucose deprivation and oxidative insults. Antioxidant activity of the bakkenolides was evaluated by cell-free bioassays. The in vitro assay results showed that all these compounds exhibited significant neuroprotective and antioxidant activities. To our knowledge, this is the first report on the neuroprotective and antioxidant activities of bakkenolides.

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Mei-Li Guo

Department of Pharmacognosy

School of Pharmacy

Second Military Medical University

325 Guohe Road

Shanghai 200433

People’s Republic of China

Phone: +86-021-2507-4576

Fax: +86-021-2507-4575

Email: mlguo@smmu.edu.cn