Am J Perinatol 2009; 26(1): 093-097
DOI: 10.1055/s-0028-1103028
© Thieme Medical Publishers

Timing of Fetal Meconium Absorption by Amnionic Macrophages

Edmund F. Funai1 , Anna T. Labowsky2 , Caroline E. Drewes2 , Harvey J. Kliman1 , 3
  • 1Section of Maternal-Fetal Medicine, Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, Connecticut
  • 2Yale College, Yale University School of Medicine, New Haven, Connecticut
  • 3Reproductive and Placental Research Unit, Yale University School of Medicine, New Haven, Connecticut
Further Information

Publication History

Publication Date:
21 November 2008 (online)

ABSTRACT

This study sought to determine the time interval from fetal defecation to membrane staining and subsequent meconium uptake by amnionic macrophages. Pieces of external membranes were fastened to Teflon rings to create a well. The amnionic surface was exposed to meconium and amniotic fluid for 1 to 48 hours and analyzed microscopically for the presence of free meconium and meconium-laden amnionic macrophages. In each of the experiments, prior to the 12-hour time point, few meconium-laden macrophages were present in the membrane layers. A significant rise in the number of meconium-laden macrophages and a concomitant increase in staining intensity was noted in the membranes at 24 and 48 hours. Contrary to previous reports, our in vitro model of meconium incorporation into placental membranes demonstrated that significant numbers of meconium-laden macrophages were only observed after 24 and 48 hours.

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Harvey J KlimanM.D. Ph.D. 

339 FMB, Department of Obstetrics/Gynecology

333 Cedar Street, New Haven, CT 06520-8063

Email: harvey.kliman@yale.edu