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Planta Med 2009; 75(12): 1293-1299
DOI: 10.1055/s-0029-1185602
Pharmacology
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Important Role of TNF-α in Inhibitory Effects of Radix Sophorae Flavescentis Extract on Vascular Restenosis in a Rat Carotid Model of Balloon Dilatation Injury

Guang Long1 , 2 , Guixue Wang1 , Linqi Ye1 , Bo Lin1 , Dangheng Wei2 , Lushan Liu2 , Li Yang1
  • 1Bioengineering College of Chongqing University and “111 Project” Laboratory of Biomechanics and Tissue Repair of Ministry of Education, Chongqing, P. R. China
  • 2College of Medicine, Nanhua University, Hengyang, P. R. China
Further Information

Publication History

received August 21, 2008 revised March 5, 2009

accepted March 16, 2009

Publication Date:
21 April 2009 (online)

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Abstract

The aim of this study was to determine the molecular mechanism underlying the inhibition of vascular restenosis by Radix Sophorae Flavescentis (RSF) extract after balloon dilatation injury. In a rat carotid model of balloon dilatation injury, the RSF extract showed a significant inhibitory effect on vascular restenosis. Immunohistochemistry showed that the expression of tumor necrosis factor-α (TNF-α) in the vascular focus was markedly reduced upon treatment with the RSF extract, whereas the expression of proliferating cell nuclear antigens (PCNA) was mostly unaffected. Colorimetric assays of methylene blue incorporation demonstrated that the proliferation of cultured vascular smooth muscle cells (VSMCs) was not inhibited with serum from rats treated with RSF extract. However, the expression of TNF-α in cultured VSMCs was significantly downregulated by serum from rats treated with RSF extract. These results suggested that RSF extract has an inhibitory effect on vascular restenosis after balloon dilatation injury which might be, in part, attributable to its inhibition of TNF-α expression.

Key words

Radix Sophorae Flavescentis - Sophora flavescens Ait. - Fabaceae - vascular restenosis - TNF‐α - balloon dilatation injury

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Guixue Wang

“111 Project” Laboratory of Biomechanics and Tissue Repair of Ministry of Education
Bioengineering College of Chongqing University

Shazhengjie 174#, Shapingba

Chongqing 400044

People's Republic of China

Phone: + 86 23 65 10 25 08

Fax: + 86 23 65 10 25 07

Email: guixuewang@yahoo.com