Download PDF
Planta Med 2009; 75(12): 1286-1292
DOI: 10.1055/s-0029-1185669
Pharmacology
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Cardioprotection and Matrix Metalloproteinase-9 Regulation of Salvianolic Acids on Myocardial Infarction in Rats

Baohong Jiang1 , Wanying Wu1 , Ming Li1 , Lingling Xu1 , Kejia Sun1 , Min Yang1 , Shuhong Guan1 , Xuan Liu1 , De-an Guo1
  • 1Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, P. R. China
Further Information

Publication History

received Sept. 2, 2008 revised March 20, 2009

accepted March 24, 2009

Publication Date:
08 May 2009 (online)

Also available at
    Permissions and Reprints

Abstract

Acute myocardial infarction (AMI) remains the leading cause of mortality in the world. Early intervention using salvianolic acids (SA) can substantially improve clinical outcomes. However, in spite of the great achievements that have been made in elucidating the protective effects of SA on AMI, the effects of SA on the contractile performance of the left ventricle (LV) and the underlying mechanism are still not so clear. In the present study, AMI was introduced by ligation of the left anterior descending coronary artery near the main pulmonary artery. Administration of SA significantly decreased infarct size, improved LV function and appearance of the myocardium and decreased myocardial malondialdehyde levels compared with the AMI group. Furthermore, treatment with SA significantly downregulated the mRNA expression level and activity of matrix metalloproteinase-9 (MMP-9), but did not regulate the tissue inhibitor of metalloproteinase-1 (TIMP-1) expression level at the infarct area. Lisinopril (an angiotensin converting enzyme inhibitor), which holds potential effects on cardioprotection, was chosen as the positive control in this study. Lisinopril elevated LV function and appearance of the myocardium, decreased malondialdehyde levels without an influence on infarct size, and regulated the MMP-9 enzyme level but not the MMP-9 mRNA and TIMP-1 protein levels. These findings suggest that early SA treatment is effective to improve LV function; and SA may exert preventative effects against myocardial remodeling after infarction.

Key words

Salvia miltiorrhiza - Labiatae - salvianolic acids - acute myocardial infarction - matrix metalloproteinase‐9 - cardioprotection

References

  • 1 Judd J T, Wexler B C. Prolyl hydroxylase and collagen metabolism after experimental myocardial infarction.  Am J Physiol. 1975;  228 212-216
    PubMedSearch in Google Scholar
  • 2 Jugdutt B I. Ventricular remodeling after infarction and the extracellular collagen matrix: when is enough enough?.  Circulation. 2003;  108 1395-1403
    CrossrefPubMedSearch in Google Scholar
  • 3 Janicki J S, Brower G L, Gardner J D, Chancey A L, Stewart Jr J A. The dynamic interaction between matrix metalloproteinase activity and adverse myocardial remodeling.  Heart Fail Rev. 2004;  9 33-42
    CrossrefPubMedSearch in Google Scholar
  • 4 Ahmed S H, Clark L L, Pennington W R, Webb C S, Bonnema D D, Leonardi A H, McClure C D, Spinale F G, Zile M R. Matrix metalloproteinases/tissue inhibitors of metalloproteinases relationship between changes in proteolytic dceterminants of matrix composition and structural, functional, and clinical manifestations of hypertensive heart disease.  Circulation. 2006;  113 2089-2096
    CrossrefPubMedSearch in Google Scholar
  • 5 Brower G L, Gardner J D, Forman M F, Murray D B, Voloshenyuk T, Levick S P, Janicki J S. The relationship between myocardial extracellular matrix remodeling and ventricular function.  Eur J Cardiothorac Surg. 2006;  30 604-610
    CrossrefPubMedSearch in Google Scholar
  • 6 Gallagher G, Menzie S, Huang Y, Jackson C, Hunyor S N. Regional cardiac dysfunction is associated with specific alterations in inflammatory cytokines and matrix metalloproteinases after acute myocardial infarction in sheep.  Basic Res Cardiol. 2007;  102 63-72
    CrossrefPubMedSearch in Google Scholar
  • 7 Rohde L E, Aikawa M, Cheng G C, Sukhova G, Solomon S D, Libby P, Pfeffer J, Pfeffer M A, Lee R T. Echocardiography-derived left ventricular end-systolic regional wall stress and matrix remodeling after experimental myocardial infarction.  J Am Coll Cardiol. 1999;  33 835-842
    CrossrefPubMedSearch in Google Scholar
  • 8 Chang P N, Mao J C, Huang S H, Ning L, Wang Z J, On T, Duan W, Zhu Y Z. Analysis of cardioprotective effects using purified Salvia miltiorrhiza extract on isolated rat hearts.  J Pharmacol Sci. 2006;  101 245-249
    CrossrefPubMedSearch in Google Scholar
  • 9 Shi C S, Huang H C, Wu H L, Kuo C H, Chang B I, Shiao M S, Shi G Y. Salvianolic acid B modulates hemostasis properties of human umbilical vein endothelial cells.  Thromb Res. 2007;  119 769-775
    CrossrefPubMedSearch in Google Scholar
  • 10 Zhang H S, Wang S Q. Salvianolic acid B from Salvia miltiorrhiza inhibits tumor necrosis factor-α(TNF-α)-induced MMP‐2 upregulation in human aortic smooth muscle cells via suppression of NAD(P)H oxidase-derived reactive oxygen species.  J Mol Cell Cardiol. 2006;  41 138-148
    CrossrefPubMedSearch in Google Scholar
  • 11 Kang E S, Lee G T, Kim B S, Kim C H, Seo G H, Han S J, Hur K Y, Ahn C W, Ha H, Jung M, Ahn Y S, Cha B S, Lee H C. Lithospermic acid B ameliorates the development of diabetic nephropathy in OLETF rats.  Eur J Pharmacol. 2008;  579 418-425
    CrossrefPubMedSearch in Google Scholar
  • 12 Lima C F, Valentao P C, Andrade P B, Seabra R M, Fernandes-Ferreira M, Pereira-Wilson C. Water and methanolic extracts of Salvia officinalis protect HepG2 cells from t-BHP induced oxidative damage.  Chem Biol Interact. 2007;  167 107-115
    CrossrefPubMedSearch in Google Scholar
  • 13 Jiang B, Zhang L, Li M, Wu W, Yang M, Wang J, Guo D A. Salvianolic acids prevent aculte doxorubicin cardiotoxiciy in mice through suppression of oxidative stress.  Food Chem Toxicol. 2008;  46 1510-1515
    CrossrefPubMedSearch in Google Scholar
  • 14 Yao Y, Wu W Y, Liu A H, Deng S S, Bi K S, Liu X, Guo D A. Interaction of salvianolic acids and notoginsengnosides in inhibition of ADP-induced platelet aggregation.  Am J Chin Med. 2008;  36 313-328
    PubMedSearch in Google Scholar
  • 15 Ling S, Dai A, Guo Z, Komesaroff P A. A preparation of herbal medicine Salvia miltiorrhiza reduces expression of intercellular adhesion molecule-1 and development of atherosclerosis in apolipoprotein E-deficient mice.  J Cardiovasc Pharmacol. 2008;  51 38-44
    CrossrefPubMedSearch in Google Scholar
  • 16 Liu A H, Guo H, Ye M, Lin Y H, Sun J H, Xu M, Guo D A. Detection, characterization and identification of phenolic acids in Danshen using high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry.  J Chromatogr A. 2007;  1161 170-182
    CrossrefPubMedSearch in Google Scholar
  • 17 Yamamoto D, Takai S, Jin D, Inagaki S, Tanaka K, Miyazaki M. Molecular mechanism of imidapril for cardiovascular protection via inhibition of MMP‐9.  J Mol Cell Cardiol. 2007;  43 670-676
    CrossrefPubMedSearch in Google Scholar
  • 18 Yamamoto D, Takai S, Miyazaki M. Prediction of interaction mode between a typical ACE inhibitor and MMP‐9 active site.  Biochem Biophys Res Commun. 2007;  354 981-984
    CrossrefPubMedSearch in Google Scholar
  • 19 Jiang B, Hattori N, Liu B, Kitagawa K, Inagaki C. Expression of swelling-and/or pH-regulated chloride channels (ClC‐2, 3, 4 and 5) in human leukemic and normal immune cells.  Life Sci. 2002;  70 1383-1394
    CrossrefPubMedSearch in Google Scholar
  • 20 Martin M U, Wesche H. Summary and comparison of the signaling mechanisms of the Toll/interleukin-1 receptor family.  Biochim Biophys Acta. 2002;  1592 265-280
    PubMedSearch in Google Scholar
  • 21 Spinale F G. Myocardial matrix remodeling and the matrix metalloproteinases: influence on cardiac form and function.  Physiol Rev. 2007;  87 1285-1342
    CrossrefPubMedSearch in Google Scholar
  • 22 Mukherjee R, Mingoia J T, Bruce J A, Austin J S, Stroud R E, Escobar G P, McClister Jr D M, Allen C M, Alfonso-Jaume M A, Fini M E, Lovett D H, Spinale F G. Selective spatiotemporal induction of matrix metalloproteinase-2 and matrix metalloproteinase-9 transcription after myocardial infarction.  Am J Physiol Heart Circ Physiol. 2006;  291 H2216-H2228
    CrossrefPubMedSearch in Google Scholar
  • 23 Karim M A, Ferguson A G, Wakim B T, Samarel A M. In vivo collagen turnover during development of thyroxine-induced left ventricular hypertrophy.  Am J Physiol. 1991;  260 C316-C326
    PubMedSearch in Google Scholar
  • 24 Cleutjens J P, Verluyten M J, Smiths J F, Daemen M J. Collagen remodeling after myocardial infarction in the rat heart.  Am J Pathol. 1995;  147 325-338
    PubMedSearch in Google Scholar
  • 25 Tao Z Y, Cavasin M A, Yang F, Liu Y H, Yang X P. Temporal changes in matrix metalloproteinase expression and inflammatory response associated with cardiac rupture after myocardial infarction in mice.  Life Sci. 2004;  74 1561-1572
    CrossrefPubMedSearch in Google Scholar
  • 26 Long C S. The role of interleukin-1 in the failing heart.  Heart Fail Rev. 2001;  6 81-94
    CrossrefPubMedSearch in Google Scholar
  • 27 Wilson E M, Moainie S L, Baskin J M, Lowry A S, Deschamps A, Mukherjee R, Guy T S, John-Sutton St MG, Gorman 3rd J H. Region- and type-specific induction of matrix metalloproteinases in post-myocardial infarction remodeling.  Circulation. 2003;  107 2857-2863
    CrossrefPubMedSearch in Google Scholar

Prof. De-an Guo

Shanghai Zhangjiang Hitech Park

Guoshoujing Road 199

Shanghai 201203

People's Republic of China

Phone: + 86 21 50 80 55 22

Fax: + 86 21 50 27 27 89

Email: gda@bjmu.edu.cn

    www.thieme-connect.de/ejournals/toc/plantamedica