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Planta Med 2009; 75(13): 1427-1430
DOI: 10.1055/s-0029-1185675
Natural Product Chemistry
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Bioassay-Guided Isolation and Identification of Desacetylmicrocolin B from Lyngbya cf. polychroa

Theresa Meickle1 , 2 , Susan Matthew3 , Cliff Ross4 , Hendrik Luesch3 , Valerie Paul1
  • 1Smithsonian Marine Station at Fort Pierce, Fort Pierce, Florida, USA
  • 2Florida Atlantic University, Boca Raton, Florida, USA
  • 3Department of Medicinal Chemistry, University of Florida, Gainesville, Florida, USA
  • 4University of North Florida, Jacksonville, Florida, USA
Further Information

Publication History

received Nov. 21, 2008 revised March 3, 2009

accepted March 26, 2009

Publication Date:
08 May 2009 (online)

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Abstract

Bioassay-guided fractionation of a non-polar extract of Lyngbya cf. polychroa resulted in the isolation of the cytotoxic desacetylmicrocolin B (1) as well as the known compounds microcolins A (2) and B (3). Compound 1 was found to inhibit the growth of HT-29 colorectal adenocarcinoma and IMR-32 neuroblastoma cells with half maximal inhibitory concentration (IC50) values of 14 nM for both cancer cell types. Microcolins A and B were found to have little activity against two strains of the marine fungus Dendryphiella salina with LD50 values above 200 µg/mL. Compounds 1, 2, and 3 were obtained by reverse-phase chromatography and their structures were determined by NMR and MS. In this paper we report the isolation, identification, and biological activity of 1.

Key words

cyanobacteria - Oscillatoriaceae - Lyngbya polychroa - microcolins - cytotoxins

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Dr. Valerie Paul

Smithsonian Marine Station at Fort Pierce

701 Seaway Drive

Fort Pierce FL 34949

USA

Phone: + 1 77 24 62 09 82

Fax: + 1 7 724 61 81 54

Email: paul@si.edu

    www.thieme-connect.de/ejournals/toc/plantamedica