Planta Med 2010; 76(7): 678-682
DOI: 10.1055/s-0029-1240649
Pharmacology
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Antiallergic Herbal Composition from Scutellaria baicalensis and Phyllostachys edulis

Dong-Seon Kim1 , Eun-Jung Son1 , Miran Kim1 , Young-Moon Heo1 , Jeong-Bum Nam1 , Jai Youl Ro2 , Sung-Sick Woo1
  • 1Unigen Inc., Cheonan, Chungnam, Korea
  • 2Department of Pharmacology, Sungkyunkwan University School of Medicine, Suwon, Korea
Further Information

Publication History

received February 20, 2009 revised October 28, 2009

accepted Nov. 1, 2009

Publication Date:
03 December 2009 (online)

Abstract

We aimed to find antiallergic agents from natural sources using mast cells activated during allergic reaction. We screened over 2000 plants for blockade of histamine release and identified two of them, S. baicalenesis and P. edulis. Bioassay-guided fractionation led to two main constituent flavonoids, baicalin from S. baicalenesis roots and isoorientin from P. edulis leaves. Based on these two compounds, two standardized extracts (SSBE and SPEE) and a combined standardized herb composition (SHC) were developed. SSBE, SPEE, and SHC remarkably inhibited histamine and leukotriene release from mast cells activated by anti-OVA/OVA binding, and SHC showed a stronger inhibition than either extract alone. SHC also showed greater inhibition potency than either aspirin or cromolyn, which are known antiallergic agents. Our results suggest that SHC reduce degranulation during mast cell activation and could be a promising candidate for the treatment of immune/allergic diseases related to mast cells.

References

  • 1 Schatz M, Leung D, Goldstein S. Consultation and referral guidelines citing the evidence: how the allergist-immunologist can help.  J Allergy Clin Immunol. 2006;  117 S495-S523
  • 2 Bielory L. Allergic and immunologic disorders of the eye. Part II: Ocular allergy.  J Allergy Clin Immunol. 2000;  106 1019-1032
  • 3 Foreman J C. Introduction to mast cells and basophils, 3rd edition. Immunopharmacology of mast cells and basophils. London; Academic Press 1993: 1-4
  • 4 Brown J M, Wilson T M, Metcalfe D D. The mast cell and allergic diseases: role in pathogenesis and implications for therapy.  Clin Exp Allergy. 2008;  38 4-18
  • 5 Metcalfe D D. Mast cells and mastocytosis.  Blood. 2008;  112 946-956
  • 6 Li H B, Jiang Y, Chen F. Separation methods used for Scutellaria baicalensis active components.  J Chromatogr B Anal Technol Biomed Life Sci. 2004;  812 277-290
  • 7 Li H Y, Sun J W, Dai S W. Study on chemical components of bamboo leaf.  Chin Med Mater. 2003;  26 562-563
  • 8 Wu S, Sun A, Liu R. Separation and purification of baicalin and wogonoside from the Chinese medicinal plant Sutellaria baicalensis Georgi by high-speed counter-current chromatography.  J Chromatogr A. 2005;  1066 243-247
  • 9 Orhan D D, Aslan M, Aktay G, Ergun E, Yesilada E, Ergun F. Evaluation of hepatoprotective effect of Gentiana olivieri herbs on subacute administration and isolation of active principle.  Life Sci. 2003;  72 2273-2283
  • 10 Ro J Y, Lee B C, Kim J Y, Chung Y J, Chung M H, Lee S K, Jo T H, Kim K H, Park Y I. Inhibitory mechanism of aloe single component (Alprogen) on mediator release in guinea pig lung mast cells activate with specific antigen-antibody reactions.  J Pharmacol Exp Ther. 2000;  292 114-121
  • 11 Grosman N. Comparison of the influence of NSAIDs with different COX-selectivity on histamine release from mast cells isolated from naïve and sensitized rats.  Int Immunopharmacol. 2007;  7 532-540
  • 12 Wells E, Jackson C G, Harper S T, Mann J, Eady R P. Characterization of primate bronchoalveolar mast cells. II. Inhibition of histamine, LTC4, and PGD2 release from primate bronchoalveolar mast cells and a comparison with rat peritoneal mast cells.  J Immunol. 1986;  137 3941-3945
  • 13 Wang X S, Lau H Y A. Histamine release from human buff coat-derived mast cells.  Int Immunopharmacol. 2007;  7 541-546

Ph.D. Sung-Sick Woo

Unigen, Inc.

200–1 Songjung-Ri

Byeongcheon-Myeon

Cheonan-Si

330–863 Chungnam

Korea

Phone: + 82 4 15 29 15 10

Fax: + 82 4 15 29 15 99

Email: sswoo@unigen.net

Ph.D. Jai Youl Ro

Department of Pharmacology
Sungkyunkwan University School of Medicine

300 Chunchun-dong Jangan-Ku

440–746 Suwon

Korea

Phone: + 82 3 12 99 61 91

Fax: + 82 3 12 99 62 09

Email: jyro426@med.skku.ac.kr